Journals / Dicle Üniversitesi Veteriner Fakültesi Dergisi / 2019 / Cilt: 12 - Sayı: 1

Investigation of Seroprevalence of Bluetongue Diseases in Sheep in the Province of Siirt

Siirt İli Koyunlarında Mavidil Hastalığı Seroprevalansının Araştırılması

Pages
53–56
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Considering the findings of the study, in addition to the measures like the establishment of quarantine protocols and vector controls, utilization of different diagnostic methods and use of vaccines specific to the virus serotypes are suggested to fight the infection.

📌 3 Key Takeaways

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Objective & Hypothesis:Bluetongue is a viral infection that is commonly encountered in domestic and wild ruminants and is transmitted by biting midges.
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Key Findings & Data:The cause of the disease is within Orbivirus genus of Reoviridae family and has 24 known serotypes.
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Conclusion & Impact:Considering the findings of the study, in addition to the measures like the establishment of quarantine protocols and vector controls, utilization of different diagnostic methods and use of vaccines specific to the virus serotypes are suggested to fight the infection.
🔬Design:Research Article
📍Scope:2017 Verileri
⚙️Variables:Koyun · ELISA · Mavidil

Abstract

Bluetongue is a viral infection that is commonly encountered in domestic and wild ruminants and is transmitted by biting midges. The cause of the disease is within Orbivirus genus of Reoviridae family and has 24 known serotypes. The infection is spread by the flies of Culicoides species. The aim of this study was to determine the serological presence of the bluetongue infection in sheep raised in various locations of Siirt province and to obtain information about the spread of the infection in the region as a whole. The study was conducted between August 2017 and February 2018, and the study consisted of 465 sheep (1-3 years old). 5 mL blood samples were taken from the jugular veins of the sheep to non-anticoagulant tubes for laboratory analyses. The blood samples were centrifuged at 3000 rpm/10 minutes and the serum were transferred to Eppendorf tubes, which were stored at -20 ºC until analysis was carried out. Determination of the Bluetongue-specific antibodies in the serum samples was performed using a commercial test kit by ELISA device. The results of the specific-antibody analysis revealed that 340 (73.12%) of 465 sheep were seropositive, while 125 (26.88%) were seronegative. As a result, the seroprevalence of Bluetongue disease was found to be considerably high in various locations of Siirt province. Considering the findings of the study, in addition to the measures like the establishment of quarantine protocols and vector controls, utilization of different diagnostic methods and use of vaccines specific to the virus serotypes are suggested to fight the infection.

Özet

Mavidil, evcil ve yabani ruminantlarda görülen ve sokucu sineklerle bulaştırılan viral bir hastalıktır. Hastalığın etkeni, Reoviridae ailesinin Orbivirus cinsi içinde yer alır ve bilinen 24 serotipi bulunmaktadır. Hastalık Culicoides türü sinekler ile yayılmaktadır. Bu araştırma, Siirt ilinin farklı lokalitelerinde yetiştirilen koyunlarda mavidil enfeksiyonun varlığının serolojik olarak belirlenmesi ve yöredeki yaygınlığı hakkında bilgi edinilmesi amacıyla yapılmıştır. Bu çalışma Ağustos 2017- Şubat 2018 tarihleri arasında Siirt ilinin farklı lokalitelerinde yetiştirilen 1-3 yaş aralığında toplam 465 koyun üzerinde gerçekleştirildi. Laboratuvar analizleri için koyunların vena jugularisinden antikoagulansız tüplere 5 mL kan örneği alındı. Alınan örnekler 3000 devirde 10 dakika santrifüje edilip serumları ependorf tüplerine aktarıldı ve analiz yapılıncaya kadar -20 ºC de saklandı. Serum örneklerinde mavidil virüsü spesifik antikor tespiti, ticari test kiti kullanılarak ELISA reader ile gerçekleştirildi. Mavidil virüs antikor tespiti için yapılan analiz sonucunda çalışmanın materyalini oluşturan 465 koyunun 340 (%73.12)’ı seropozitif bulunurken, 125 (% 26.88)’i seronegatif tespit edildi. Sonuç olarak; Siirt ilinin farklı lokalitelerinde mavidil hastalığının seroprevalansı oldukça yüksek tespit edilmiştir. Çalışma verileri dikkate alındığında teşhiste farklı tanı yöntemlerinin kullanılması, hastalıkla mücadelede karantina ve vektör kontrolüne yönelik önlemler yanında, virüs serotiplerine spesifik aşıların kullanılması gerektiği düşünülmektedir.

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Cite This Article

ÇELİK, Ö. Y., ŞAHİN, T. (2019). Investigation of Seroprevalence of Bluetongue Diseases in Sheep in the Province of Siirt. Dicle Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 12 - Sayı: 1, 53–56. https://ajindex.com/articles/149643

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