Journals / Herkes için Spor ve Rekreasyon Dergisi / 2026 / Volume: 8 Issue: 1

Inflammation Following Yo-Yo Level-1 Test with Circadian Timing: The Effect of Time-of-Day on Biomarker Responses

Ayşegül Yapıcı(PAMUKKALE ÜNİVERSİTESİ)0000-0003-4243-5507
Engin Güneş Atabaş(PAMUKKALE ÜNİVERSİTESİ, SPOR BİLİMLERİ VE TEKNOLOJİSİ ARAŞTIRMA VE UYGULAMA MERKEZİ)0000-0002-9942-0835
Süleyman Gönülateş(PAMUKKALE ÜNİVERSİTESİ)0000-0003-3330-7685
Pages
—
Publication Date
2026-03-30
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, aerobic endurance performance and glycolytic system ac-tivation in soccer players increase during evening hours associated with circadian rhythms.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of this study is to investigate the acute effects of the Yo-Yo Intermittent Recovery Test (Level-1) administered in the morning and evening hours on performance, metabolic hormones, and inflammation parameters in soccer players.
📊
Key Findings & Data:Thirteen voluntary male soc-cer players (mean age: 23.39 ± 2.5 years) from Tavasbirlik Sports Club, competing in the Denizli Super Amateur League, participated in the study.
💡
Conclusion & Impact:In conclusion, aerobic endurance performance and glycolytic system ac-tivation in soccer players increase during evening hours associated with circadian rhythms.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:soccer · circadian rhythm · yo-yo IR1

Abstract

The aim of this study is to investigate the acute effects of the Yo-Yo Intermittent Recovery Test (Level-1) administered in the morning and evening hours on performance, metabolic hormones, and inflammation parameters in soccer players. Thirteen voluntary male soc-cer players (mean age: 23.39 ± 2.5 years) from Tavasbirlik Sports Club, competing in the Denizli Super Amateur League, participated in the study. The Yo-Yo IR1 test was admin-istered to the athletes with a one-week interval in the morning (09:00–11:00) and evening (17:00–19:00). Data were collected via venous blood samples taken Pre-Test (Pre), Post-Test (Post), and 2 Hours Post-Test (2h-Post), along with lactate measurements taken 3 minutes after the completion of the test. According to the findings, the total distance covered in the evening measurements (1655.38 m) was statistically significantly higher than in the morning measurements (1363.07 m) (p<0.05). Similarly, evening post-exercise lactate val-ues (8.40 mmol/L) were significantly higher than morning values (6.39 mmol/L). Regard-ing inflammation parameters, it was determined that morning baseline CRP levels were higher than evening levels, whereas IL-1β levels showed an increase during the evening recovery phase. In conclusion, aerobic endurance performance and glycolytic system ac-tivation in soccer players increase during evening hours associated with circadian rhythms.

Özet

Bu çalışmanın amacı, futbolcularda sabah ve akşam saatlerinde uygulanan Yo-Yo Aralıklı Toparlanma Testi (Seviye-1)'in performans, metabolik hormonlar ve inflamasyon parametreleri üzerindeki akut etkilerini incelemektir. Çalışmaya, Denizli Süper Amatör Ligi’nde mücadele eden Tavasbirlik Spor Kulübü’nden 13 gönüllü erkek futbolcu (yaş ortalaması: 23,39 ± 2,5 yıl) katılmıştır. Sporculara Yo-Yo IR1 testi, bir hafta arayla sabah (09.00-11.00) ve akşam (17.00-19.00) saatlerinde uygulanmıştır. Veriler; Test Öncesi (TÖ), Test Sonrası (TS) ve Testten 2 Saat Sonra (TS2) alınan venöz kan örnekleri ve testin tamamlanmasından 3 dakika sonra alınan laktat ölçümleri aracılığıyla toplanmıştır. Elde edilen bulgulara göre, akşam ölçümlerinde kat edilen toplam mesafe (1655,38 m), sabah ölçümlerine (1363,07 m) kıyasla istatistiksel olarak anlamlı düzeyde daha yüksek bulunmuştur (p<0,05). Benzer şekilde, akşam egzersiz sonrası laktat değerleri (8,40 mmol/L), sabah değerlerinden (6,39 mmol/L) anlamlı derecede daha yüksek bulunmuştur. İnflamasyon parametreleri açısından; sabah bazal CRP düzeylerinin akşam düzeylerinden daha yüksek olduğu, IL-1β düzeylerinin ise akşam toparlanma evresinde artış gösterdiği tespit edilmiştir. Sonuç olarak, futbolcularda aerobik dayanıklılık performansı ve glikolitik sistem aktivasyonunun, sirkadiyen ritimle ilişkili olarak akşam saatlerinde artış gösterdiği belirlenmiştir.

🔖

Cite This Article

Yapıcı, A., Atabaş, E. G., Gönülateş, S. (2026). Inflammation Following Yo-Yo Level-1 Test with Circadian Timing: The Effect of Time-of-Day on Biomarker Responses. Herkes için Spor ve Rekreasyon Dergisi, Volume: 8 Issue: 1. https://doi.org/10.56639/jsar.1830568

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (25)

  1. Akgün, N. (1994). Egzersiz ve spor fizyolojisi. Ege Üniversitesi Basımevi.
  2. Bangsbo, J., Iaia, F. M., & Krustrup, P. (2008). The Yo-Yo intermittent recovery test: A useful tool for evaluation of physical performance in intermittent sports. Sports Medicine, 38(1), 37–51.
  3. Bougrine, H., Ammar, A., Trabelsi, K., Belgacem, A., Salem, A., Chtourou, H., Jahrami, H., & Souissi, N. (2024). The effect of last meal Suhoor timing on diurnal variations in cognitive performance during Ramadan fasting among female athletes. Frontiers in Nutrition, 11, 1365128. https://doi.org/10.3389/fnut.2024.1365128
  4. Çamkerten, D. (2016). Elit güreşçilerde su ilavesinin bazı hematolojik parametrelere etkisi [Yayımlanmamış Yüksek Lisans Tezi]. Selçuk Universitesi.
  5. Çolakoğlu, F. (2014). 8 haftalık koş-yürü egzersizinin sedanter orta yaşlı obez bayanlarda fizyolojik, motorik ve somatotip değerleri üzerine etkisi. Gazi Eğitim Fakültesi Dergisi, 23(3).
  6. Doruk, M. (2019). Sabah akşam yapılan Yo-Yo aralıklı toparlanma testinin (Seviye-1) metabolik hormonlar ve biyokimyasal parametreler üzerine akut etkisi [Yayımlanmamış Yüksek Lisans Tezi]. Pamukkale Universitesi.
  7. Eniseler, N. (2010). Bilimin ışığında futbol antrenmanı. Birleşik Matbaacılık.
  8. Hasani, S., Hashemi, M., Eskandari-Nasab, E., & Naderi, M. (2012). A functional polymorphism in the miR-146a gene is associated with the risk of childhood acute lymphoblastic leukemia: A preliminary report. Tumor Biology.
  9. Jafari, A., & Kazemi, F. (2025). The impact of acute morning versus evening high-intensity interval training on circulating irisin levels and metabolic parameters in overweight postmenopausal women. Experimental Gerontology, 208, 112821. https://doi.org/10.1016/j.exger.2025.112821
  10. Kaynar, Ö., Kıyıcı, F., Öztürk, N., & Bakan, E. (2015). Elit güreşçilerde akut egzersizin plazma lipit düzeylerine etkisi. Beden Eğitimi ve Spor Bilimleri Dergisi, 17, 33.
  11. Kılıç-Toprak, E., Yapici, A., Kilic-Erkek, O., Koklu, Y., Tekin, V., Alemdaroglu, U., & Bor-Kucukatay, M. (2015). Acute effects of Yo-Yo intermittent recovery test level 1 (Yo-YoIR1) on hemorheological parameters in female volleyball players. Clinical Hemorheology and Microcirculation, 60(2), 191–199.
  12. Koca, H. B., Yıldırım, Ö., Işık, T., Bal, T., & Tan, F. (2017). 8 haftalık aerobik egzersizin sedanter genç kadınlarda inflamatuar sitokinler üzerine etkisi. Genel Tıp Dergisi, 27(1), 1–5.
  13. Kurdiova, T., Balaz, M., Vician, M., Maderova, D., Vlcek, M., Valkovic, L.,. Ukropcova, B. (2014). Effects of obesity, diabetes and exercise on FNDC5 gene expression and irisin release in human skeletal muscle and adipose tissue: In vivo and in vitro studies. The Journal of Physiology, 592(5), 1091–1107.
  14. Mirghani, S. J., Alinejad, H. A., Azarbayjani, M. A., Mazidi, A., & Mirghani, S. A. (2014). Influence of strength, endurance and concurrent training on the lipid profile and blood testosterone and cortisol response in young male wrestlers. Baltic Journal of Health and Physical Activity, 6(1), 7–16.
  15. Özçelik, O., Algül, S., Deniz, M., & Baydaş, F. (2018). Sabah ve gece yapılan aerobik egzersizin antrenmanlı deneklerde serum irisin düzeyine etkilerinin belirlenmesi. Journal of Sports and Performance Researches, 9(1), 25–34.
  16. Patlar, S. (2010). The effect of acute and chronic submaximal exercise on leukocyte and leukocyte subgroups. Isokinetics and Exercise Science, 18(3), 145–148.
  17. Pérez-Castillo, Í. M., Rueda, R., Bouzamondo, H., López-Chicharro, J., & Mihic, N. (2023). Biomarkers of post-match recovery in semi-professional and professional football (soccer). Frontiers in Physiology, 14, 1167449. https://doi.org/10.3389/fphys.2023.1167449
  18. Pilat, C., Krüger, K., Frech, T., & Mooren, F. C. (2017). Effects of acute and 4-week submaximal exercise on leukocyte and leukocyte subgroups. Journal of Immunological Methods, 440, 58–63.
  19. Reilly, T., Atkinson, G., & Waterhouse, J. (2000). Biological rhythms and exercise. Oxford University Press.
  20. Tsuchiya, Y., Ando, D., Takamatsu, K., & Goto, K. (2015). Resistance exercise induces a greater irisin response than endurance exercise. Metabolism, 64(9), 1042–1050.
  21. Wahl, P., Zinner, C., Achtzehn, S., Bloch, W., & Mester, J. (2010). Effect of different mechanical loading conditions on the acute hormonal response to resistance exercise. Journal of Strength and Conditioning Research. 111(7), 1405-1413. https://doi.org/10.1007/s00421-010-1767-1
  22. Xu, B., & Guo, S. (2025). Daytime eccentric exercise and its impact on inflammatory markers and antioxidant defenses in physically active young men. Frontiers in Immunology, 16, 1655034. https://doi.org/10.3389/fimmu.2025.1655034
  23. Yan, B., & Li, L. (2025). Morning sprint interval training produces greater physical performance adaptations than evening training in soccer players. Scientific Reports, 15, 6154. https://doi.org/10.1038/s41598-025-89821-4
  24. Younesian, A., Mohammadion, M., & Rahnama, N. (2004). Haematology of professional soccer players before and after a 90-minute match. Cellular & Molecular Biology Letters, 9(2).
  25. Kurdiova, T., Balaz, M., Vician, M., Maderova, D., Vlcek, M., Valkovic, L.,. Ukropcova, B. (2014). Effects of obesity, diabetes and exercise on FNDC5 gene expression and irisin release in human skeletal muscle and adipose tissue: In vivo and in vitro studies. The Journal of Physiology, 592(5), 1091–1107.