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Journals / Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi / 2026 / Volume: 44 Issue: 2

Daidzein alleviates isoproterenol-induced cardiac injury in rats via NF-kB pathway inhibition

Vadivelan Ramachandran(JSS College of Pharmacy,Ooty)0000-0001-7927-4554
Vikash Vaikunth(JSS College of Pharmacy)0009-0007-9813-8129
Tharani Mohanasundaram(JSS College of Pharmacy)0000-0001-7465-1525
Ruchı Tıwarı(Pranveer Singh Institute of Technology (Pharmacy))0000-0001-6897-3472
Harshini Magesh(JSS College of Pharmacy)0009-0001-8557-0197
Pages
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Publication Date
2024-06-01
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This research shows that daidzein treatment in vivo reduces Iso-induced myocardial damage by preventing NF-kB activation, which may therefore reduce the release of inflammatory cytokines.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The current study set out to explore the anti-inflammatory effects of Daidzein (Dz) on the isoproterenol (Iso)-induced myocardial infarction (MI) in male Wistar rats.
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Key Findings & Data:Iso(80 mg/kg) was injected subcutaneously into rats to cause MI for two days at intervals of 24 hours.
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Conclusion & Impact:This research shows that daidzein treatment in vivo reduces Iso-induced myocardial damage by preventing NF-kB activation, which may therefore reduce the release of inflammatory cytokines.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Daidzein · Isoproterenol · Myocardial Injury

Abstract

The current study set out to explore the anti-inflammatory effects of Daidzein (Dz) on the isoproterenol (Iso)-induced myocardial infarction (MI) in male Wistar rats. Iso(80 mg/kg) was injected subcutaneously into rats to cause MI for two days at intervals of 24 hours. ELISA kits were used to measure the amounts of TNF-α & IL-6 in cardiac tissues. The quantity of NF-kB in the cytosol and nuclei was measured using a western blot approach. H&E staining was used to identify histological cardiac changes. In rats with Iso-induced myocardial infarction, Dz therapy shrunk the size of the infarct and reduced histological anomalies in the myocardium. Dz significantly lowered cardiac pro-inflammatory cytokine levels and prevented NF-kB nuclear translocation in MI-damaged rats, substantially reversing Iso-induced damage. This research shows that daidzein treatment in vivo reduces Iso-induced myocardial damage by preventing NF-kB activation, which may therefore reduce the release of inflammatory cytokines.

Özet

The current study set out to explore the anti-inflammatory effects of Daidzein (Dz) on the isoproterenol (Iso)-induced myocardial infarction (MI) in male Wistar rats. Iso(80 mg/kg) was injected subcutaneously into rats to cause MI for two days at intervals of 24 hours. ELISA kits were used to measure the amounts of TNF-α & IL-6 in cardiac tissues. The quantity of NF-kB in the cytosol and nuclei was measured using a western blot approach. H&E staining was used to identify histological cardiac changes. In rats with Iso-induced myocardial infarction, Dz therapy shrunk the size of the infarct and reduced histological anomalies in the myocardium. Dz significantly lowered cardiac pro-inflammatory cytokine levels and prevented NF-kB nuclear translocation in MI-damaged rats, substantially reversing Iso-induced damage. This research shows that daidzein treatment in vivo reduces Iso-induced myocardial damage by preventing NF-kB activation, which may therefore reduce the release of inflammatory cytokines.

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Cite This Article

Ramachandran, V., Vaikunth, V., Mohanasundaram, T., Tıwarı, R., Magesh, H. (2024). Daidzein alleviates isoproterenol-induced cardiac injury in rats via NF-kB pathway inhibition. Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi, Volume: 44 Issue: 2. https://doi.org/10.52794/hujpharm.1338923

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