Journals / Experimental and Applied Medical Science / 2025 / Cilt: 6 Sayı: 4

Is round cell density in semen a factor affecting pregnancy in infertility treatment?

İnfertilite tedavisinde semendeki yuvarlak hücre yoğunluğu gebeliği etkileyen bir faktör müdür?

Selin Kenan(gaziantep şehir hastanesi)0000-0001-7957-7328
Ayşe Gülüşür(Gaziantep şehir hastanesi)0009-0000-9713-2402
Çiğdem Karaca(GAZIANTEP ISLAM SCIENCE AND TECHNOLOGY UNIVERSITY)0000-0003-2106-2422
Dilşad Arısoy Demir(gaziantep şehir hastanesi)0000-0002-9632-1700
Özlem Akay(GAZİANTEP İSLAM BİLİM VE TEKNOLOJİ ÜNİVERSİTESİ)0000-0002-9539-7252
Pages
326–337
Publication Date
2025-12-31
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: As a result of our study, it was observed that round cell cell density did not affect pregnancy outcomes in unexplained infertility and male-related infertility cases.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Purpose: Round cells seen during spermiogram evaluation are cells that have the potential to affect pregnancy outcomes.
📊
Key Findings & Data:Methods: A retrospective study was conducted in terms of round cell in men who applied to Gaziantep Cengiz Gökçek Gynecology and Obstetrics and Pediatrics Hospital, Assisted Reproductive Treatment Center for in vitro fertilization and intrauterine insemination between 02/01/2022 and 01/01/2023.
💡
Conclusion & Impact:Conclusion: As a result of our study, it was observed that round cell cell density did not affect pregnancy outcomes in unexplained infertility and male-related infertility cases.
🔬Design:Araştırma Makalesi
📍Scope:2022 Verileri
⚙️Variables:yuvarlak hücre · açıklanamayan infertilite · erkek kaynaklı infertilite

Abstract

Purpose: Round cells seen during spermiogram evaluation are cells that have the potential to affect pregnancy outcomes. In this study, we evaluated the effect of round cell density on pregnancy outcome in semen samples of patients admitted to our assisted reproductive treatment center for male-induced infertility and unexplained infertility. Methods: A retrospective study was conducted in terms of round cell in men who applied to Gaziantep Cengiz Gökçek Gynecology and Obstetrics and Pediatrics Hospital, Assisted Reproductive Treatment Center for in vitro fertilization and intrauterine insemination between 02/01/2022 and 01/01/2023. A total of 98 cases were analyzed: 75 cases with a diagnosis of male-induced infertility and 23 cases with a diagnosis of unexplained infertility. Statistical analysis was performed with the help of IBM SPSS Statistics 25.0 program and p<0.05 values were accepted as significant. Results: In unexplained infertility and male-related infertility patients, no statistically significant difference was observed in terms of round cell cell density according to pregnancy outcome (p=0.140, p=0.919). Conclusion: As a result of our study, it was observed that round cell cell density did not affect pregnancy outcomes in unexplained infertility and male-related infertility cases.

Özet

Amaç: Spermiyogram değerlendirmesi sırasında görülen yuvarlak hücreler, gebelik sonuçlarını etkileme potansiyeline sahip hücrelerdir. Bu çalışmada, erkek kaynaklı infertilite ve açıklanamayan infertilite nedeniyle üremeye yardımcı tedavi merkezimize başvuran hastaların semen örneklerinde yuvarlak hücre yoğunluğunun gebelik sonuçları üzerindeki etkisi değerlendirildi. Yöntem: Gaziantep Cengiz Gökçek Kadın Doğum ve Çocuk Hastalıkları Hastanesi Üremeye Yardımcı Tedavi Merkezine 02/01/2022 ile 01/01/2023 tarihleri arasında in vitro fertilizasyon ve intrauterin inseminasyon için başvuran erkeklerde yuvarlak hücre açısından retrospektif bir çalışma yapıldı. Erkek kaynaklı infertilite tanısı alan 75 vaka ve açıklanamayan infertilite tanısı alan 23 vaka olmak üzere toplam 98 vaka analiz edilmiştir. İstatistiksel analiz IBM SPSS Statistics 25.0 programı yardımıyla yapıldı ve p<0.05 değerleri anlamlı olarak kabul edildi. Bulgular: Açıklanamayan infertilite ve erkeğe bağlı infertilite hastalarında, gebelik sonucuna göre yuvarlak hücre yoğunluğu açısından istatistiksel olarak anlamlı bir fark gözlenmedi (p=0.140, p=0.919). Sonuç: Çalışmamızın sonucunda açıklanamayan infertilite ve erkek ilişkili infertilite olgularında yuvarlak hücre yoğunluğunun gebelik sonuçlarını etkilemediği görüldü.

🔖

Cite This Article

Kenan, S., Gülüşür, A., Karaca, Ç., Demir, D. A., Akay, Ö. (2025). Is round cell density in semen a factor affecting pregnancy in infertility treatment?. Experimental and Applied Medical Science, Cilt: 6 Sayı: 4, 326–337. https://doi.org/10.46871/eams.1713019

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (20)

  1. Organization WH. WHO laboratory manual for the examination and processing of human semen. vol. 6. 2021. https://doi.org/10.1016/j.fertnstert.2021.11.037
  2. Raperport C, Desai J, Qureshi D et al. The definition of unexplained infertility: a systematic review. BJOG 2024;131:880–97. https://doi.org/10.1111/1471-0528.17697
  3. Massarotti C, Fraire-Zamora JJ, Liperis G, et al. Understanding and addressing unexplained infertility: from diagnosis to treatment. Human Reproduction 2024;39:1155–9. https://doi.org/10.1093/humrep/deae054
  4. Kumar N, Singh AK. Trends of male-factor infertility, an important cause of infertility: A review of literature. J Hum Reprod Sci 2015;8:191–6. DOI: 10.4103/0974-1208.170370
  5. Johanisson E, Campana A, Luthi R et al. Evaluation of “round cells” in semen analysis: A comparative study. Hum Reprod Update 2000;6:404–12. https://doi.org/10.1093/humupd/6.4.404.
  6. Patil PS, Humbarwadi RS, Patil AD et al Immature germ cells in semen–correlation with total sperm count and sperm motility. J Cytol 2013;30:185–9. DOI: 10.4103/0970-9371.117682
  7. . Emilie L., Allan P., Anne-Bine S. Recent decline in sperm motility among donor candidates at a sperm bank in Denmark. Human Reproduction, Volume 39, Issue 8, August 2024, Pages 1618–1627. https://doi.org/10.1093/humrep/deae115
  8. Levine, H., Niels J., Anderson M. Et al. Temporal trends in sperm count: a systematic review and meta-regression analysis. Human reproduction update 23.6 2017; 646-659. DOI: https://doi.org/10.1093/humupd/dmx022
  9. Vadim O., Andre B., Reza K. Semen microbiota are dramatically altered in men with abnormal sperm parameters. 2024.Scientific Reports volume 14, Article number: 1068 https://doi.org/10.1038/s41598-024-51686-4
  10. Fernando T., Walter D. Increased exfoliation of immature germ cells detected in semen analysis routine and its clinical significance. (2022). Archivio Italiano Di Urologia E Andrologia, 94(4), 519-520. https://doi.org/10.4081/aiua.2022.4.519
  11. Ariagno J, Curi S, Mendeluk G et al. Shedding of immature germ cells. Arch Androl 2002;48:127–31. https://doi.org/10.1080/014850102317267436
  12. Bollwein H, Petschow K, Weber F et al. The incidence of agglutination and its influence on sperm quality and fertility of boar semen. Berl Munch Tierarztl Wochenschr 2004;117:327–33. DOI: https://doi.org/10.1136/bmj.305.6854.609
  13. Brazdova A. Study of immunological properties of sperm and seminal plasma antigens: anti-seminal and anti-sperm antibodies in female immune infertility: characterization of targeted proteins 2014. PhD Thesis. Université Pierre et Marie Curie-Paris VI; Vysoká škola chemicko-technologická (Praze) DOI: https://theses.hal.science/tel-01037917/
  14. Sigman M, Lopes L. Round cells in diagnostic semen analysis: a guide for laboratories and clinicians. British Journal of Biomedical Science 2022; 79, 10129. DOI:https://doi.org/10.3389/bjbs.2021.10129
  15. Son J., Samuel R., Gale B. Et al. Separation of sperm cells from samples containing high concentrations of white blood cells using a spiral channel. Biomicrofluidics, 2017; 11(5). https://doi.org/10.1063/1.4994548
  16. Alvarez JG, Sharma RK, Ollero M, et al. Increased DNA damage in sperm from leukocytospermic semen samples as determined by the sperm chromatin structure assay. Fertil Steril 2002;78:319–29. DOI: https://doi.org/10.1136/bmj.305.6854.609
  17. Iommiello VM, Albani E, Di Rosa A et al. Ejaculate oxidative stress is related with sperm DNA fragmentation and round cells. Int J Endocrinol 2015;2015:321901. https://doi.org/10.1155/2015/321901
  18. Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 2003;79:829–43. https://doi.org/10.1016/S0015-0282(02)04948-8
  19. Lackner JE, Märk I, Sator K et al. M. Effect of leukocytospermia on fertilization and pregnancy rates of artificial reproductive technologies. Fertil Steril 2008;90:869–71. https://doi.org/10.1016/j.fertnstert.2007.07.1310
  20. Rakesh S., Sajal G., Ashok A. et al. Relevance of Leukocytospermia and Semen Culture and Its True Place in Diagnosing and Treating Male Infertility. World J Mens Health.2021 Jun 9;40(2):191–207 DOI: 10.5534/wjmh.210063