Journals / Eurasian Journal of Agricultural Research / 2025 / Volume: 9 Issue: 2

Antimicrobial Effects of Gelatin Films Enriched with Lemon Peel Extract in Beef Meatballs during Storage

Pages
—
Publication Date
2025-12-31
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results indicate significant differences (P < 0.05) in the microbial counts.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study aimed to examine the antimicrobial effects of gelatin films enriched with lemon peel extract on beef meatballs during storage.
📊
Key Findings & Data:To do this, three groups were set up: the control group (C), which had no gelatin film wrapping; the second group (G1), wrapped with gelatin film supplemented with 2% lemon peel extract; and the third group (G2), wrapped with gelatin film supplemented with 4% lemon peel extract.
💡
Conclusion & Impact:The results indicate significant differences (P < 0.05) in the microbial counts.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Edible films · Lemon peel · Quality

Abstract

This study aimed to examine the antimicrobial effects of gelatin films enriched with lemon peel extract on beef meatballs during storage. To do this, three groups were set up: the control group (C), which had no gelatin film wrapping; the second group (G1), wrapped with gelatin film supplemented with 2% lemon peel extract; and the third group (G2), wrapped with gelatin film supplemented with 4% lemon peel extract. Microbiological analysis was conducted to assess the quality of beef meatballs over 11 days of storage. The results indicate significant differences (P < 0.05) in the microbial counts. The initial total mesophilic bacteria count was 1.59 log CFU/g, increasing to 6.87, 4.12 and 3.37 log CFU/g in groups C, G1 and G2, respectively, by the end of storage. Similarly, the initial total Enterobacteriaceae count was 1.28 log CFU/g, rising to 4.27, 3.46 and 3.07 log CFU/g in groups C, G1 and G2, respectively. The initial total psychrophilic count was 1.17 log CFU/g, increasing during storage to 6.74, 4.72 and 4.11 log CFU/g in groups C, G1 and G2, respectively. Lemon peel treatment, particularly at 4%, effectively reduced bacterial growth and preserved product quality, indicating its potential as a natural antimicrobial packaging material. Thus, gelatin films enriched with lemon peel can effectively extend the shelf life of beef meatballs.

🔖

Cite This Article

Elsheikh, W., Uçak, İ. (2025). Antimicrobial Effects of Gelatin Films Enriched with Lemon Peel Extract in Beef Meatballs during Storage. Eurasian Journal of Agricultural Research, Volume: 9 Issue: 2. https://ajindex.com/articles/1481472

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (17)

  1. Abd-Alzahra, E. H. & Makki G. A. 2025. Effect of adding lemon peel extract fortified with gelatin on the preservation of beef meatballs by refrigeration and some microbial properties. University of Thi-Qar Journal of Agricultural Research, 4(1), 215-220.
  2. Barbosa L.N., Rall V.L.M., Fernandes A.A.H., Ushimaru P.I., Probst I.S. & Fernandes A., 2009. Essential oils against foodborne pathogens and spoilage bacteria in minced meat. Foodborne Pathogens and Disease 6, 725–728.
  3. Benmebarek I. E., Gonzalez-Serrano D. J., Aghababaei F., Ziogkas D., Garcia-Cruz R., Boukhari A.,. & Hadidi, M. 2024. Optimising the microwave-assisted hydrothermal extraction of pectin from tangerine by-product and its physicochemical, structural, and functional properties. Food Chemistry: X, 23, 101615.
  4. Etxabide A., Leceta I., Cabezudo S., Guerrero P. & de la Caba, K. 2016. Sustainable fish gelatin films: From food processing waste to compost. ACS Sustainable Chemistry & Engineering, 4(9), 4626-4634.
  5. Giannenas I., Sidiropoulou E., Bonos E., Christaki E. & Florou-Paneri P. 2020. The history of herbs, medicinal and aromatic plants, and their extracts: past, current situation, and future perspectives. In: Florou-Paneri, P., Christaki, E., Giannenas, I. (Eds.), Feed Additives: Aromatic Plants and Herbs in Animal Nutrition and Health. Academic Press. Elsevier, pp. 1–18.
  6. Gómez-Urios C., Vinas-Ospino A., Puchades-Colera P., Blesa J., López-Malo D., Frígola A. & Esteve M. J. 2023. Choline chloride-based natural deep eutectic solvents for the extraction and stability of phenolic compounds, ascorbic acid, and antioxidant capacity from Citrus sinensis peel. LWT, 177, 114595.
  7. Huang L.Y., Wang Y., Li R.T., Wang Q.L., Dong J., Wang J.Y., Lu S.L. 2021. Thyme essential oil and sausage diameter effects on biogenic amine formation and microbiological load in smoked horse meat sausage. Food Biosci. 40, 1000885.
  8. Hui X., Wan Y., Dong H., Peng J., Wu W., Yang X. & He Q. 2023. A promising insight into the inhibition of lipid oxidation, protein degradation, and biogenic amine accumulation in postmortem fish: Functional glazing layers of modified bio-polymer. LWT, 177, 114575.
  9. ICMSF. 1982. Microorganisms in foods. Their significance and methods of enumeration (2nd ed.). Univ. Toronto Pres.
  10. Isvand A., Karimaei S. & Amini M. 2024. Assessment of chitosan coating enriched with Citrus limon essential oil on the quality characteristics and shelf life of beef meat during cold storage. International Journal of Food Microbiology, 423, 110825.
  11. Liu C., Xu S., Liu X., Wang W., Liao W., Yang X. & He Q. 2025. Preservation of Beef with Limonene-Rich Citrus Peel Extracts: Antioxidant, Antimicrobial and Textural Benefits. Foods, 14(20), 3506.
  12. Lucera A., Mastromatteo M., Sinigaglia M., Corbo M.R. 2009. Combined effects of thymol, carvacrol, and grapefruit seed extract on lipid oxidation and color stability of poultry meat preparations. Int. J. Food Sci. Technol. 44, 2256–2267.
  13. Magalhães D., Vilas-Boas A.A., Teixeira P. & Pintado M. 2023. Functional ingredients and additives from lemon by-products and their applications in food preservation: A review. Foods, 12(5), 1095.
  14. Pesavento G., Calonico C., Bilia A.R., Barnabei M., Calesini F., Addona R., Mencarelli L., Carmagnini L., Di Martino M.C. & Lo Nostro A. 2015. Antibacterial activity of Oregano, Rosmarinus and Thymus essential oils against Staphylococcus aureus and Listeria monocytogenes in beef meatballs. Food Control, 54, 188–199.
  15. Soni A., Gurunathan K., Mendiratta S.K., Talukder S., Jaiswal R.K. & Sharma H. 2018. Effect of essential oils incorporated edible film on quality and storage stability of chicken patties at refrigeration temperature (4±1 °C). Journal of food science and technology, 55(9), 3538-3546.
  16. Uçak İ. & Khalily R. 2022. Effects of different solvent extractions on the total phenolic content and antioxidant activity of lemon and orange peels. Eurasian Journal of Food Science and Technology, 6(1), 23-28.
  17. Benmebarek I. E., Gonzalez-Serrano D. J., Aghababaei F., Ziogkas D., Garcia-Cruz R., Boukhari A.,. & Hadidi, M. 2024. Optimising the microwave-assisted hydrothermal extraction of pectin from tangerine by-product and its physicochemical, structural, and functional properties. Food Chemistry: X, 23, 101615.