Journals / Eurasian Journal of Agricultural Research / 2025 / Volume: 9 Issue: 2

Influence of Red-Light Emitting Diode in Shoot Tip Culture of Banana (Musa acuminata × Musa balbisiana) 'Cardava'

Pages
—
Publication Date
2025-12-31
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results show that the explant exposed to red-light at 24 hours obtained the highest final biomass, increment of initial and final biomass, lowest percent contamination, lowest tissue browning, and highest percent tissue greening of the shoot tip culture of banana.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Under in vitro plant culture, light is the essential environmental cue that affects plant photosynthesis and photomorphogenesis.
📊
Key Findings & Data:The experiment was arranged in (CRD) Completely Randomized Design and replicated 3 times with 3 treatments in each replication.
💡
Conclusion & Impact:The results show that the explant exposed to red-light at 24 hours obtained the highest final biomass, increment of initial and final biomass, lowest percent contamination, lowest tissue browning, and highest percent tissue greening of the shoot tip culture of banana.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Banana · In vitro culture · Red-light emitting diode

Abstract

Under in vitro plant culture, light is the essential environmental cue that affects plant photosynthesis and photomorphogenesis. Fluorescent and high-pressure sodium lamps are common sources of light. Nevertheless, the wide spectral distribution of these traditional lights leads to the ineffective availability of some spectral qualities linked to photomorphogenesis. As a consideration, this study was conducted to test the effectivity of red-light emitting diode in the shoot tip culture of banana. The experiment was arranged in (CRD) Completely Randomized Design and replicated 3 times with 3 treatments in each replication. The treatment 1 – Control (Natural Lighting), T2 – Red Light Emitting Diode (LED) at 8 of hours exposure, and T3 - Red LED at 24 hours of exposure. The colored lighting especially the Red LED greatly influenced the in vitro culture of banana. The results show that the explant exposed to red-light at 24 hours obtained the highest final biomass, increment of initial and final biomass, lowest percent contamination, lowest tissue browning, and highest percent tissue greening of the shoot tip culture of banana. However, the natural lighting obtained the lightest weight of final biomass weight, initial and final increment, highest tissue browning, lowest percentage of tissue greening, and highest contamination.

🔖

Cite This Article

Maximo, R. V. (2025). Influence of Red-Light Emitting Diode in Shoot Tip Culture of Banana (Musa acuminata × Musa balbisiana) 'Cardava'. Eurasian Journal of Agricultural Research, Volume: 9 Issue: 2. https://ajindex.com/articles/1481470

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (22)

  1. Bantis F., Ouzounis T. & Radoglou K. 2021. Artificial LED lighting enhances plant photosynthesis and biomass accumulation. Plants,10(3), 526. Doi: 10.3390/plants10030526
  2. Batista D.S., Felipe S.H.S., Silva T.D., de Castro K.M., Mamedes-Rodrigues T.C., Miranda N.A., Ríos-Ríos A.M., Faria D.V., Fortini E.A. & Chagas K. 2018. Light quality in plant tissue culture: Does it matter? Vitr. Cell. Dev. Biol. Plant.; 54, 195-215. Doi: 10.1007/s11627-018-9902-5.
  3. Cavallaro V., Arena C., Giuffrida F. & Toscano S. 2023. Effects of spectral quality and light quantity of LEDs on in vitro plant growth and morphogenesis. Agronomy, 13(4), 1072. Doi: 10.3390/agronomy13041072
  4. Chen M., Chory J. & Fankhauser C. 2004. Light signal transduction in higher plants. Annu. Rev. Genet. 38, 87-117. Doi: 10.1146/annurev.genet.38.072902.092259.
  5. Cioć M., Szewczyk A., Żupnik M., Kalisz A. & Pawłowska B. 2017. LED lighting affects plant growth, morphogenesis and phytochemical contents of Myrtus communis L. in vitro. Plant Cell, Tissue and Organ Culture (PCTOC), Doi:10.1007/s11240-017-1340-2
  6. Edmale U.V., Huang S.C., Zander M., Cole B.J., Hetzel J., Ljung K. & Chory J. 2016. Cryptochromes interact directly with PIFs to control plant growth in limiting blue light. Cell. 164, 233–245
  7. He C., Davies F. T. & Lacey R. E. 2020. Light quality affects in vitro plant growth and morphogenesis: Physiological and biochemical mechanisms. PeerJ, 8, e9180. Doi: 10.7717/peerj.9180
  8. Holderbaum D. F., Kon T., Kudo T. & Guerra, M. P. 2010. Enzymatic Browning, Polyphenol Oxidase Activity, and Polyphenols in Four Apple Cultivars: Dynamics during Fruit Development. HortScience horts, 45(8), 1150-1154. Retrieved Sep 21, 2024, doi: 10.21273/HORTSCI.45.8.1150
  9. Johkan M. K., Shoji F., Goto S., Hahida N. & Yoshihara T. 2012. Effect of green light wavelength and intensity on photomorphogenesis and photosynthesis in Lactuca sativa, Environmental and Experimental Botany, 75, 128-133.
  10. Kapoor S., Raghuvanshi R., Bhardwaj P., Sood H., Saxena S. &. Chaurasia O.P. 2018. Influence of light quality on growth, secondary metabolites production and antioxidant activity in callus culture of Rhodiola imbricata Edgew, Journal of Photochemistry and Photobiology B: Biology, 183, 258–265.
  11. Köhler K.H., Dörfler M. & Göring H. 1980. The influence of light on the cytokinin content of Amaranthus seedlings, Biologia Plantarum, 22(2), 128-134.
  12. Kozai T. 2016. Why LED lighting for urban agriculture? In: Kozai T., Fujiwara K., Runkle E., editors. LED Lighting for Urban Agriculture. Springer; Singapore.
  13. Li C.X., Xu Z.G., Dong R.Q., Chang S.X., Wang L.Z., Khalil-Ur-Rehman M. & Tao J.M. 2017. An RNA-Seq analysis of grape plantlets grown in vitro reveals different responses to blue, green, red led light, and white fluorescent light. Front. Plant Sci.
  14. Muneer S., Kim E.J. & Park J.S. 2014. Influence of green, red and blue light emitting diodes on multiprotein complex proteins and photosynthetic activity under different light intensities in lettuce leaves (Lactuca sativa L.) Int. J. Mol. Sci. 15, 4657-4670. Doi: 10.3390/ijms15034657.
  15. Park Y. & Runkle E. S. 2021. Far-red radiation promotes growth of seedlings by increasing leaf expansion and photosynthetic capacity. Horticulture Research, 8(1), 1-11. Doi: 10.1038/s41438-021-00529-4
  16. Pawłowska B., Żupnik M., Szewczyk-Taranek B. & Cioć M. 2022. Impact of LED light spectra and photoperiod on plant growth, morphogenesis, and photoreceptor activity in in vitro cultures. Plants, 11(4), 529. Doi: 10.3390/plants11040529
  17. Permadi N., Nurzaman M., Alhasnawi A. N., Doni F., & Julaeha E. 2023. Managing Lethal Browning and Microbial Contamination in Musa spp. Tissue Culture: Synthesis and Perspectives. Horticulturae. MDPI. Doi: 10.3390/horticulturae9040453
  18. Poothong, S., Sirikantaramas, S., & Mekprasert, C. 2023. Light quality regulates hormone signaling and shoot proliferation during in vitro plant propagation. Plant Cell, Tissue and Organ Culture, 153(2), 237-250. Doi: 10.1007/s11240-023-02465-3
  19. Shirani S., Mahdavi F. & Maziah M. 2009. Morphological abnormality among regenerated shootsof banana and plantain (Musa spp.) after in vitro multiplication with TDZ and BAP from excised shoot tips. Afr. J. Biotechnol. 8(21), 5755-5761.
  20. Stefanova M., et al. 2024. LED light spectrum affects biomass accumulation and stomatal characteristics of in vitro propagated plants. Journal of Central European Agriculture, 25(1), 45–58.
  21. Vendrame W. A., Moore K. K. & Carvalho, V. S. 2022. In vitro growth responses of ornamental bananas (Musa spp.) as affected by light sources. Horticulturae, 8(2), 92. Doi: 10.3390/horticulturae8020092
  22. Yu L., Song C., Sun L., LI L., XU Z. & Tang C. 2020. Effects of light-emitting diodes on tissue culture plantlets and seedlings of rice (Oryza sativa L.). Journal of Integrative Agriculture, 19(7), 1743-1754. doi:10.1016/S2095-3119(19)62793-0