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Journals / Biyolojik Çeşitlilik ve Koruma / 2024 / Volume: 17 Issue: 3

A comparative haematological research on Bufo bufo (Linnaeus, 1758) and B. verrucosissimus (Pallas, 1814) in Türkiye

Türkiye'deki Bufo bufo (Linnaeus, 1758) ve B. verrucosissimus (Pallas, 1814) üzerine karşılaştırmalı bir hematolojik araştırma

Cantekin Dursun(RECEP TAYYIP ERDOGAN UNIVERSITY, FACULTY OF ARTS AND SCIENCES)0000-0001-7766-1470
Nagihan Demirci(RECEP TAYYIP ERDOGAN UNIVERSITY, FACULTY OF ARTS AND SCIENCES)0009-0008-7657-4760
Yaren Akyüz(RECEP TAYYIP ERDOGAN UNIVERSITY, FACULTY OF ARTS AND SCIENCES)0009-0009-3516-2310
Serkan Gül(RECEP TAYYIP ERDOGAN UNIVERSITY, FACULTY OF ARTS AND SCIENCES)0000-0002-0372-7462
Nurhayat Özdemir(RECEP TAYYIP ERDOGAN UNIVERSITY, FACULTY OF ARTS AND SCIENCES)0000-0002-3880-5846
Pages
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Publication Date
2024-12-15
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was found that female individuals had larger values for measurement of blood cell parameters.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, it is aimed to contribute to the systematic situation of Bufo bufo and B. verrucosissimus by comparing some blood parameters for the first time.
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Key Findings & Data:For that, fieldwork was done in Lake Borçka Karagöl, Artvin for B. verrucosissimus and Lake Uzungöl, Trabzon for B. bufo in 2022.
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Conclusion & Impact:It was found that female individuals had larger values for measurement of blood cell parameters.
🔬Design:Research Article
📍Scope:2022 Verileri
⚙️Variables:blood cell · Common Toad · Caucasian Toad

Abstract

In this study, it is aimed to contribute to the systematic situation of Bufo bufo and B. verrucosissimus by comparing some blood parameters for the first time. For that, fieldwork was done in Lake Borçka Karagöl, Artvin for B. verrucosissimus and Lake Uzungöl, Trabzon for B. bufo in 2022. A total of 6 adult individuals (3 males, 3 females) were captured per species. For each individual, 1 ml blood sample was acquired from spontaneously pulsating heart ventricle and blood smears were prepared. Under a microscope, 40 randomly chosen erythrocytes from each smear were measured to acquire 9 distinct blood cell parameters. Additionally, erythrocyte and leukocyte counts were calculated using Neubauer hemacytometer. It was found that female individuals had larger values for measurement of blood cell parameters. Mean erythrocyte and leucocyte numbers were higher in the blood of B. bufo than B. verrucosissimus but there was not a significant difference between species for erythrocyte and leukocyte counts. In females, erythrocytes were larger and narrower in B. verrucosissimus than B. bufo species. Alike, nuclei were larger and narrower as observed in B. verrucosissimus, similar to erythrocytes. However, B. bufo had larger and wider erythrocytes but larger and narrower nuclei in males. Given the differences between sexes, these characters thought as not diagnostic. In addition, PCA analysis showed overlapped position of species in morphospace and supported the weak discrimination power of the characters. Our findings will contribute to the future studies as a reference source for basic blood parameters.

Özet

Bu çalışmada, bazı kan parametrelerini ilk kez karşılaştırılarak Bufo bufo ve B. verrucosissimus türlerinin sistematik durumuna katkı sağlanması hedeflenmiştir. Bu amaçla, 2022 yılı içerisinde B. verrucosissimus için Artvin Borçka Karagöl ve B. bufo için Trabzon Uzungöl mevkisinde saha çalışması yapılmıştır. Tür başına toplam 6 yetişkin birey (3 erkek, 3 dişi) yakalanmıştır. Her birey için atmaya devam eden kalp ventrikülünden 1 ml kan örneği alınmış ve yayma preparatlar hazırlanmıştır. Mikroskop altında, 9 farklı kan hücresi parametresine ait veriyi elde etmek için her yaymadan rastgele seçilen 40 eritrosit ölçülmüştür. Ayrıca Neubauer hemasitometresi kullanılarak eritrosit ve lökosit sayıları hesaplanmıştır. Kan hücresi parametrelerinin ölçüm sonuçlarına göre dişilerin erkeklerden daha büyük değerlere sahip olduğu belirlenmiştir. B. bufo türünün kanındaki ortalama eritrosit ve lökosit sayıları B. verrucosissimus türüne göre daha fazla olsa da eritrosit ve lökosit sayıları açısından türler arasında anlamlı bir farklılık tespit edilmemiştir. Dişiler için eritrositler B. verrucosissimus türünde B. bufo türüne göre daha büyük ve daha dar şekle sahiptir. Benzer şekilde, B. verrucosissimus türünde çekirdekler, eritrositlerde gözlendiği gibi daha büyük ve daha dar şekillidir. Öte yandan, erkekler için B. bufo türüne ait eritrositler daha büyük ve daha geniş şekilli fakat çekirdekleri daha büyük ve daha dar şekillidir. Cinsiyetler arasındaki farklılıklar göz önüne alındığında, bu karakterlerin diyagnostik olmadığı düşünülmüştür. Ayrıca PCA analizi türlerin morfouzaydaki örtüşen konumlarını göstermiş ve karakterlerin zayıf ayırt etme gücünü desteklemiştir. Bulgularımız temel kan parametreleri için referans kaynağı olarak gelecek çalışmalara katkı sağlayacaktır.

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Cite This Article

Dursun, C., Demirci, N., Akyüz, Y., Gül, S., Özdemir, N. (2024). A comparative haematological research on Bufo bufo (Linnaeus, 1758) and B. verrucosissimus (Pallas, 1814) in Türkiye. Biyolojik Çeşitlilik ve Koruma, Volume: 17 Issue: 3. https://doi.org/10.46309/biodicon.2024.1426045

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