SavedAdd your journal

Journals / Biyolojik Çeşitlilik ve Koruma / 2025 / Volume: 18 Issue: 3

Antiproliferative and cytotoxic activity of Papaver somniferum seed oil on human breast cancer cell lines

Papaver somniferum tohum yağının insan meme kanseri hücre hatları üzerinde antiproliferatif ve sitotoksik aktivitesi

Ersin Yücel(Eskisehir Technical University, Faculty of Sciences, 26470 Tepebaşı / Eskişehir–Türkiye)0000-0001-8274-7578
Didem Turgut Coşan(ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-8488-6405
Dilge Yücel(ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-6595-8231
Birgül Büyükkıdan(ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0001-9677-9028
İbrahim Uğur Çalış(MUĞLA SITKI KOÇMAN ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ BİYOLOJİ ANABİLİM DALI)0000-0003-2907-2035
Bartu Badak(ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0003-3465-8719
Pages
—
Publication Date
2025-12-15
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

P. somniforum seed oil may be a natural anticancer agent to treat breast cancer, but more studies are needed on this subject.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Background: This study aimed to determine the antiproliferative and cytotoxic activities of Papaver somniferum seed oil on human breast cancer (MDA-MB-231) cell lines under in vitro conditions.
📊
Key Findings & Data:In the study, control group, DMSO (0.1%) applied group and 5 different concentrations of Poppy seed oil applied group three main treatment groups were created.
💡
Conclusion & Impact:P. somniforum seed oil may be a natural anticancer agent to treat breast cancer, but more studies are needed on this subject.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:breast cancer · cytotoxicity · Papaver somniferum

Abstract

Background: This study aimed to determine the antiproliferative and cytotoxic activities of Papaver somniferum seed oil on human breast cancer (MDA-MB-231) cell lines under in vitro conditions. Methods: Poppy seed oil extracted by cold press method were subjected to compositional analysis using GC-MS. In the study, control group, DMSO (0.1%) applied group and 5 different concentrations of Poppy seed oil applied group three main treatment groups were created. Cell viability was investigated by the trypan blue method. Some pharmacokinetic properties and toxicity profiles of the identified molecules were predicted using a Swiss ADME online server and ProTox-II webserver. Results: The effect of Poppy seed oil on breast cancer cells increased relatively starting from the lowest concentration (87 µg/ml), and it was determined that the effect became more pronounced after a concentration of 350 µg/ml. Except Hexadecanoic acid and (E)-9- Octadecanoic acid, all other compounds were predicted to be non-hepatotoxic, non-carcinogenic, non-cytotoxic, non-immunotoxic and non-mutagenic. All compounds investigated are orally absorbable, suitable for dermal trans-distribution, have high gastrointestinal absorption, however, Hexadecanoic acid is permeable to the blood-brain barrier (BBB), while the other 4 compounds are not. The bioavailability values of all compounds investigated can be considered to be very well absorbed by the body. Conclusion: In this study, it was determined that P. somniferum seed oil has antiproliferative and cytotoxic activity on breast cancer cells in vitro. P. somniforum seed oil may be a natural anticancer agent to treat breast cancer, but more studies are needed on this subject.

Özet

Amaç: Bu çalışmada, Papaver somniferum tohum yağının insan meme kanseri (MDA-MB-231) hücre hatları üzerindeki antiproliferatif ve sitotoksik aktivitelerinin in vitro koşullarda belirlenmesi amaçlanmıştır. Metod: Soğuk pres yöntemiyle ekstrakte edilen haşhaş tohumu yağı, GC-MS kullanılarak kompozisyon analizine tabi tutulmuştur. Çalışmada, kontrol grubu, DMSO (%0,1) uygulanan grup ve 5 farklı konsantrasyonda haşhaş tohumu yağı uygulanan grup olmak üzere üç ana tedavi grubu oluşturulmuştur. Hücre canlılığı tripan mavisi yöntemi ile araştırılmıştır. Tanımlanan moleküllerin bazı farmakokinetik özellikleri ve toksisite profilleri, bir İsviçre ADME çevrimiçi sunucusu ve ProTox-II web sunucusu kullanılarak tahmin edilmiştir. Bulgular: Haşhaş tohumu yağının meme kanseri hücreleri üzerindeki etkisi en düşük konsantrasyondan (87 µg/ml) başlayarak nispeten artmış ve etkinin 350 µg/ml konsantrasyonundan sonra daha belirgin hale geldiği belirlenmiştir. Heksadekanoik asit ve (E)-9- Oktadekanoik asit hariç, diğer tüm bileşiklerin hepatotoksik, kanserojen, sitotoksik, immünotoksik ve mutajenik olmadığı öngörülmüştür. İncelenen tüm bileşikler oral yoldan emilebilir, dermal trans-dağılım için uygundur ve yüksek gastrointestinal emilime sahiptir; ancak Heksadekanoik asit kan-beyin bariyerine (BBB) geçirgenken, diğer 4 bileşik geçirgen değildir. İncelenen tüm bileşiklerin biyoyararlanım değerlerinin vücut tarafından çok iyi emildiği düşünülebilir. Sonuç: Bu çalışmada, P. somniferum tohum yağının in vitro meme kanseri hücreleri üzerinde antiproliferatif ve sitotoksik aktiviteye sahip olduğu belirlenmiştir. P. somniforum tohum yağının meme kanserini tedavi etmek için doğal bir antikanser ajanı olabileceği, ancak bu konuda daha fazla çalışmaya ihtiyaç duyulduğu belirtilmiştir.

🔖

Cite This Article

Yücel, E., Coşan, D. T., Yücel, D., Büyükkıdan, B., Çalış, İ. U., Badak, B. (2025). Antiproliferative and cytotoxic activity of Papaver somniferum seed oil on human breast cancer cell lines. Biyolojik Çeşitlilik ve Koruma, Volume: 18 Issue: 3. https://doi.org/10.46309/biodicon.2025.1786862

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (28)

  1. Tétényi, P. (2010). Opium poppy (Papaver somniferum): botany and horticulture. Horticultural Reviews. John Wiley & Sons, Ltd, 373-408.
  2. Ngernsaengsaruay, C., Leksungnoen, N., Chanton, P., Andriyas, T., Thaweekun, P., Rueansri, S.,. & Hauyluek, W. (2023). Morphology, taxonomy, anatomy, and palynology of the opium poppy (Papaver somniferum L.) cultivation in Northern Thailand. Plants, 12(11), 2105. https://doi.org/10.3390/plants12112105
  3. Chaudhary, S. S., Aslam, M., & Siddiqui, A. (2019). Tukhm khashkhaash (poppy seeds): A unani drug of multitudinous potential. J. Drug Deliv. Ther, 9, 528-533. https://doi.org/10.22270/jddt.v9i2.2560
  4. Aruna, K., & Sivaramakrishnan, V. M. (1992). Anticarcinogenic effects of some Indian plant products. Food and Chemical Toxicology, 30(11), 953-956. https://doi.org/10.1016/0278-6915(92)90180-S
  5. Carlin, M. G., Dean, J. R., & Ames, J. M. (2020). Opium alkaloids in harvested and thermally processed poppy seeds. Frontiers in Chemistry, 8, 737. https://doi.org/10.3389/fchem.2020.00737
  6. Fantuzzi, G. (2005). Adipose tissue, adipokines, and inflammation. Journal of Allergy and clinical immunology, 115(5), 911-919. https://doi.org/10.1016/j.jaci.2005.02.023
  7. Staels, B., Dallongeville, J., Auwerx, J., Schoonjans, K., Leitersdorf, E., & Fruchart, J. C. (1998). Mechanism of action of fibrates on lipid and lipoprotein metabolism. Circulation, 98(19), 2088-2093. https://doi.org/10.1161/01.CIR.98.19.2088
  8. Furuhashi, M., & Hotamisligil, G. S. (2008). Fatty acid-binding proteins: role in metabolic diseases and potential as drug targets. Nature reviews Drug discovery, 7(6), 489-503. https://doi.org/10.1038/nrd2589
  9. Torra, I. P., Chinetti, G., Duval, C., Fruchart, J. C., & Staels, B. (2001). Peroxisome proliferator-activated receptors: from transcriptional control to clinical practice. Current Opinion in Lipidology, 12(3), 245-254. https://doi.org/10.1097/00041433-200106000-00002
  10. van Raalte, D. H., Li, M., Pritchard, P. H., & Wasan, K. M. (2004). Peroxisome proliferator-activated receptor (PPAR)-α: a pharmacological target with a promising future. Pharmaceutical research, 21, 1531-1538. https://doi.org/10.1023/B:PHAM.0000041444.06122.8d
  11. Rouessac, F., & Rouessac, A. (2013). Chemical analysis: modern instrumentation methods and techniques. John Wiley & Sons.
  12. Freshney, R. I. (2015). Culture of animal cells: a manual of basic technique and specialized applications. John Wiley & Sons.
  13. Daina, A., Michielin, O., & Zoete, V. (2017). SwissADME: a free web tool to evaluate pharmacokinetics, drug-likeness and medicinal chemistry friendliness of small molecules. Scientific reports, 7(1), 42717. https://doi.org/10.1038/srep42717
  14. Banerjee, P., Kemmler, E., Dunkel, M., & Preissner, R. (2024). ProTox 3.0: a webserver for the prediction of toxicity of chemicals. Nucleic Acids Research, 52(W1), W513-W520. https://doi.org/10.1093/nar/gkae303
  15. Yücel, E. (2008). Tıbbi Bitkiler 1. Cetemenler Digital, Eskişehir.
  16. Güler, D. A., Aydın, A., Koyuncu, M., & Parmaksız, İ. (2016). Anticancer activity of Papaver somniferum.Journal of the Turkish Chemical Society Section A: Chemistry, 3(3), 349-366. https://doi.org/10.18596/jotcsa.43273.
  17. Sharopov, F., Valiev, A., Gulmurodov, I., Sobeh, M., Satyal, P., & Wink, M. (2018). Alkaloid content, antioxidant and cytotoxic activities of various parts of Papaver somniferum. Pharmaceutical Chemistry Journal, 52, 459-463. https://doi.org/10.1007/s11094-018-1839-9
  18. Gach, K., Wyrębska, A., Fichna, J., & Janecka, A. (2011). The role of morphine in regulation of cancer cell growth. Naunyn-Schmiedeberg's archives of pharmacology, 384, 221-230. https://doi.org/10.1007/s00210-011-0672-4
  19. Yücel, E. (2014). Türkiye’de Yetişen Tıbbi Bitkiler Tanıma Klavuzu. Türmatsan, İstanbul.
  20. Şengün, İ. Y., Yücel, E., Öztürk, B., & Kılıç, G. (2020). Haşhaş (Papaver somniforum) çeşitlerinin tohum yağlarinin yağ asidi kompozisyonu, toplam fenolik madde miktari, antioksidan ve antimikrobiyal aktiviteleri. GIDA: The Journal of Food, 45(5). https://doi.org/10.15237/gida.GD20061
  21. Khare, C. P. (2008). Indian medicinal plants: an illustrated dictionary. Springer Science & Business Media.
  22. Millar, J. S. (2013). Novel benefits of peroxisome proliferator-activated receptors on cardiovascular risk. Current opinion in lipidology, 24(3), 233-238. https://doi.org/10.1097/MOL.0b013e3283613a7d
  23. Chinetti, G., Lestavel, S., Bocher, V., Remaley, A. T., Neve, B., Torra, I. P.,. & Staels, B. (2001). PPAR-α and PPAR-γ activators induce cholesterol removal from human macrophage foam cells through stimulation of the ABCA1 pathway. Nature medicine, 7(1), 53-58. https://doi.org/10.1038/83348
  24. Owada, Y. (2008). Fatty acid binding protein: localization and functional significance in the brain. The Tohoku journal of experimental medicine, 214(3), 213-220. https://doi.org/10.1620/tjem.214.213
  25. Maeda, K., Cao, H., Kono, K., Gorgun, C. Z., Furuhashi, M., Uysal, K. T.,. & Hotamisligil, G. S. (2005). Adipocyte/macrophage fatty acid binding proteins control integrated metabolic responses in obesity and diabetes. Cell metabolism, 1(2), 107-119. https://doi.org/10.1016/j.cmet.(2004.12.008
  26. Ngernsaengsaruay, C., Leksungnoen, N., Chanton, P., Andriyas, T., Thaweekun, P., Rueansri, S.,. & Hauyluek, W. (2023). Morphology, taxonomy, anatomy, and palynology of the opium poppy (Papaver somniferum L.) cultivation in Northern Thailand. Plants, 12(11), 2105. https://doi.org/10.3390/plants12112105
  27. Chinetti, G., Lestavel, S., Bocher, V., Remaley, A. T., Neve, B., Torra, I. P.,. & Staels, B. (2001). PPAR-α and PPAR-γ activators induce cholesterol removal from human macrophage foam cells through stimulation of the ABCA1 pathway. Nature medicine, 7(1), 53-58. https://doi.org/10.1038/83348
  28. Maeda, K., Cao, H., Kono, K., Gorgun, C. Z., Furuhashi, M., Uysal, K. T.,. & Hotamisligil, G. S. (2005). Adipocyte/macrophage fatty acid binding proteins control integrated metabolic responses in obesity and diabetes. Cell metabolism, 1(2), 107-119. https://doi.org/10.1016/j.cmet.(2004.12.008