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Journals / Turkish Journal of Veterinary and Animal Sciences / 2005 / Cilt: 29 - Sayı: 3

The Effects of Fenfluramine on Blood and Tissue Serotonin (5-Hydroxytryptamine) Levels in Rats

İbrahim PİRİNÇCİ
Ahmet ATEŞŞAHİN
İzzet KARAHAN
Pages
857–863
Publication Date
2005-03-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, while fenfluramine was determined to decrease brain and plasma 5-HT levels generally, it increased the levels of stomach 5-HT.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of this study was to investigate the effect of fenfluramine (FEN) administered at single and repeated doses on brain, stomach and plasma serotonin (5-Hydroxytryptamine, 5-HT) levels in rats.
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Key Findings & Data:FEN was injected intraperitoneally at single doses (1, 3 and 10 µmol kg-1) and given orally at repeated doses (1.25, 5 and 10 mg kg-1) for 21 days.
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Conclusion & Impact:In conclusion, while fenfluramine was determined to decrease brain and plasma 5-HT levels generally, it increased the levels of stomach 5-HT.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Fenfluramine · rat · brain

Abstract

The aim of this study was to investigate the effect of fenfluramine (FEN) administered at single and repeated doses on brain, stomach and plasma serotonin (5-Hydroxytryptamine, 5-HT) levels in rats. FEN was injected intraperitoneally at single doses (1, 3 and 10 µmol kg-1) and given orally at repeated doses (1.25, 5 and 10 mg kg-1) for 21 days. The tissues and plasma samples were collected 20, 40, 80 and 160 minutes after the single dose administrations and 4 h after the last dose administration in repeated experiments. In addition, the rats treated with the dose of 1.25 mg kg-1 were maintained until 2 and 8 weeks and the tissues were taken at these times. The 5-HT levels in the tissue and plasma were measured by using high performance liquid chromatography (HPLC). The levels of brain 5-HT were decreased at the single FEN doses when compared with the control group. At the repeated experiments, although no effect was observed at the dose of 1.25 mg kg-1; decreases were determined in brain 5-HT levels at the high doses. The levels of 5-HT in the stomach were found to increase in both single and repeated administrations. Decreases in levels of plasma 5-HT were determined in all experimental groups. In conclusion, while fenfluramine was determined to decrease brain and plasma 5-HT levels generally, it increased the levels of stomach 5-HT.

Özet

The aim of this study was to investigate the effect of fenfluramine (FEN) administered at single and repeated doses on brain, stomach and plasma serotonin (5-Hydroxytryptamine, 5-HT) levels in rats. FEN was injected intraperitoneally at single doses (1, 3 and 10 µmol kg-1) and given orally at repeated doses (1.25, 5 and 10 mg kg-1) for 21 days. The tissues and plasma samples were collected 20, 40, 80 and 160 minutes after the single dose administrations and 4 h after the last dose administration in repeated experiments. In addition, the rats treated with the dose of 1.25 mg kg-1 were maintained until 2 and 8 weeks and the tissues were taken at these times. The 5-HT levels in the tissue and plasma were measured by using high performance liquid chromatography (HPLC). The levels of brain 5-HT were decreased at the single FEN doses when compared with the control group. At the repeated experiments, although no effect was observed at the dose of 1.25 mg kg-1; decreases were determined in brain 5-HT levels at the high doses. The levels of 5-HT in the stomach were found to increase in both single and repeated administrations. Decreases in levels of plasma 5-HT were determined in all experimental groups. In conclusion, while fenfluramine was determined to decrease brain and plasma 5-HT levels generally, it increased the levels of stomach 5-HT.

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Cite This Article

PİRİNÇCİ, İ., ATEŞŞAHİN, A., KARAHAN, İ. (2005). The Effects of Fenfluramine on Blood and Tissue Serotonin (5-Hydroxytryptamine) Levels in Rats. Turkish Journal of Veterinary and Animal Sciences, Cilt: 29 - Sayı: 3, 857–863. https://ajindex.com/articles/146357

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