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Journals / Adıyaman Üniversitesi Sağlık Bilimleri Dergisi / 2023 / Cilt: 9 Sayı: 3

Preliminary in vitro assessment of cytotoxic and genotoxic effects of avocado (Persea Americano) oil in breast cancer cell line (MCF-7)

Avokado (Persea Americano) yağının meme kanseri hücre hattı (MCF-7) üzerindeki sitotoksik ve genotoksik etkilerinin in vitro ön değerlendirmesi

Ayça Aktaş Şüküroğlu(MERSİN ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ, ECZACILIK MESLEK BİLİMLERİ BÖLÜMÜ, FARMASOTİK TOKSİKOLOJİ ANABİLİM DALI)0000-0003-3069-7905
Pages
162–168
Publication Date
2023-12-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Regular use of avocado oil as a dietary supplement has been shown to have a protective effect.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aim: Toxicological evaluation is required to understand the safety of avocado (Persea Americana) oil for use as a food supplement.
📊
Key Findings & Data:In this study, cytotoxic and genotoxic effects of avocado oil in MCF-7 cell line were evaluated.
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Conclusion & Impact:Regular use of avocado oil as a dietary supplement has been shown to have a protective effect.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Avokado yağı · Sitotoksisite · Genotoksisite

Abstract

Aim: Toxicological evaluation is required to understand the safety of avocado (Persea Americana) oil for use as a food supplement. In this study, cytotoxic and genotoxic effects of avocado oil in MCF-7 cell line were evaluated. Materials and Methods: In this study, the MCF-7 was exposed to avocado oil (1, 10, 25 and 100 ppm) for 24, 48 and 72 hrs to assess the cytotoxic and genotoxic effects. Results: IC50 of avocado oil were found to be 68.1, 62.8 and 64.3 ppm for 24, 48 and 72 hrs, respectively. There was a statistically significant decrease in cell polferation between the control and exposed groups (p<0.05). Micronucleus frequency was significantly increased compared with negative control (p<0.005). Conclusion: Results of the study, avocado oil had cytotoxic and genotoxic effects in a time and concentration dependent manner. Regular use of avocado oil as a dietary supplement has been shown to have a protective effect.

Özet

Amaç: Avokado (Persea Americana) yağının gıda takviyesi olarak kullanımında güvenliğinin anlaşılması için toksikolojik değerlendirilme yapılması gerekmektedir. Planlanan bu çalışmada avokado yağının MCF-7 hücre hattındaki sitotoksik ve genotoksik etkileri değerlendirilmiştir. Gereç ve Yöntem: Bu çalışmada MCF-7 hücre hattı, avokado yağına (1, 10, 25 ve 100 ppm) ile 24, 48 ve 72 maruz bırakılarak sitotoksik ve genotoksik etkisi değerlendirilmiştir. Bulgular: Avokado yağının IC50 değerleri 24, 48 ve 72 saat için sırasıyla; 68.1, 62.8 ve 64.3 ppm olarak bulunmuştur. Avokado yağının bütün maruziyet sürelerinde kontrol grubu ile maruziyet grupları arasında hücre poliferasyonundaki azalma istatiksel olarak anlamlı bulunmuştur (p<0,05). Avokado yağına maruziyetine bağlı mikroçekirdek frekansında, tüm dozlarda negatif kontrole göre önemli artış görülmüştür (p<0,005). Sonuç: Çalışmanın sonucunda avokado yağının MCF-7 hücre hattında zamana ve konstrasyona bağımlı olarak sitotoksik ve genotoksik etkilerinin olduğu görülmüştür. Avokado yağının gıda takviyesi olarak düzenli kullanılması sonucunda koruyucu etkisinin olabileceği görülmüştür.

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Cite This Article

Şüküroğlu, A. A. (2023). Preliminary in vitro assessment of cytotoxic and genotoxic effects of avocado (Persea Americano) oil in breast cancer cell line (MCF-7). Adıyaman Üniversitesi Sağlık Bilimleri Dergisi, Cilt: 9 Sayı: 3, 162–168. https://doi.org/10.30569/adiyamansaglik.1332125

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