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Journals / Adıyaman Üniversitesi Sağlık Bilimleri Dergisi / 2026 / Cilt: 11 Sayı: 2

Selenium in the treatment of colon cancer: Investigation of effect on epithelial mesenchymal transition

Kolon kanseri tedavisinde selenyum: Epitel mezenkimal geçiş üzerindeki etkisinin araştırılması

Sibel Güneş Bağış(Eskişehir Osmangazi Üniversitesi, Hücresel Tedavi ve Kök Hücre Üretim, Uygulama ve Araştırma Merkezi (ESTEM))0000-0003-0846-1170
Özlem Tomsuk(ESKISEHIR OSMANGAZI UNIVERSITY, CELLULAR TREATMENT AND STEM CELL PRODUCTION APPLICATION AND RESEARCH CENTER)0000-0001-7201-686X
Fulya Büge Ergen(ESKISEHIR OSMANGAZI UNIVERSITY, CELLULAR TREATMENT AND STEM CELL PRODUCTION APPLICATION AND RESEARCH CENTER)0000-0001-8089-9033
Ayla Eker Sarıboyacı(ESKISEHIR OSMANGAZI UNIVERSITY, CELLULAR TREATMENT AND STEM CELL PRODUCTION APPLICATION AND RESEARCH CENTER)0000-0003-4536-9859
Onur Uysal(ESKISEHIR OSMANGAZI UNIVERSITY, CELLULAR TREATMENT AND STEM CELL PRODUCTION APPLICATION AND RESEARCH CENTER)0000-0001-6800-5607
Pages
103–111
Publication Date
2025-08-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: SLM exhibited cytotoxic effects and modulated EMT-related gene expression, suggesting potential therapeutic implications in CRC.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: This study aimed to investigate the therapeutic effect of seleno-L-methionine (SLM), a selenium (Se) compound, on colorectal cancer (CRC) cells through epithelial-mesenchymal transition (EMT)-related transcription factors (ZEB1 and ZEB2).
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Key Findings & Data:Materials and Methods: The human colon carcinoma cell line (Caco-2) was treated with different concentrations of SLM (15.6–250 nmol/L) for 24 hours.
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Conclusion & Impact:Conclusion: SLM exhibited cytotoxic effects and modulated EMT-related gene expression, suggesting potential therapeutic implications in CRC.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Kolon kanseri · Selenyum · Epitelyal mezenkimal geçiş

Abstract

Aim: This study aimed to investigate the therapeutic effect of seleno-L-methionine (SLM), a selenium (Se) compound, on colorectal cancer (CRC) cells through epithelial-mesenchymal transition (EMT)-related transcription factors (ZEB1 and ZEB2). Materials and Methods: The human colon carcinoma cell line (Caco-2) was treated with different concentrations of SLM (15.6–250 nmol/L) for 24 hours. Cell viability was assessed using the MTT assay, live/dead rate and morphology were evaluated by Calcein-AM/Propidium Iodide staining, and ZEB1 and ZEB2 gene expressions were analyzed by qRT-PCR. Results: SLM decreased cell viability in a dose-dependent manner (IC50: 31.25 nmol/L). Live/dead analysis revealed a significant increase in non-viable cells in the SLM-treated group compared to controls, although clear apoptotic morphology was not consistently observed. qRT-PCR analysis revealed a significant downregulation of ZEB2 and a slight reduction in ZEB1 expression. Conclusion: SLM exhibited cytotoxic effects and modulated EMT-related gene expression, suggesting potential therapeutic implications in CRC.

Özet

Amaç: Bu çalışma, bir selenyum bileşiği olan seleno-L-metiyoninin (SLM), kolorektal kanser (KRK) hücrelerinde epitel-mezenkimal geçiş (EMT) ile ilişkili transkripsiyon faktörleri ZEB1 ve ZEB2 üzerindeki terapötik potansiyelini araştırmayı amaçlamıştır. Gereç ve Yöntem: İnsan kolon karsinomu hücre hattı (Caco-2) 24 saat boyunca farklı konsantrasyonlarda SLM (15,6-250 nmol/L) ile tedavi edildi. Hücre canlılığı MTT testi ile değerlendirildi, canlı/ölü oranı ve morfoloji kalsein-AM/propidyum iyodür boyama ile değerlendirildi ve ZEB1 ve ZEB2 gen ekspresyonları qRT-PCR ile analiz edildi. Bulgular: SLM, hücre canlılığını doza bağımlı olarak azalttı (IC50: 31,25 nmol/L). Canlı/ölü boyama sonuçları, SLM uygulanan gruplarda anlamlı düzeyde artmış ölü hücre oranı gösterdi; ancak tipik apoptotik morfolojik özellikler tutarlı şekilde gözlemlenmedi. qRT-PCR analizi, ZEB2'nin önemli bir şekilde azaldığını ve ZEB1 ekspresyonunda hafif bir azalma olduğunu ortaya koydu. Sonuç: SLM, Caco-2 hücrelerinde sitotoksik etkiler göstermiş ve EMT ile ilişkili gen ekspresyonunu modüle etmiştir. Bu bulgular, SLM’nin CRC’de terapötik potansiyele sahip olabileceğini düşündürmektedir.

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Cite This Article

Bağış, S. G., Tomsuk, Ö., Ergen, F. B., Sarıboyacı, A. E., Uysal, O. (2025). Selenium in the treatment of colon cancer: Investigation of effect on epithelial mesenchymal transition. Adıyaman Üniversitesi Sağlık Bilimleri Dergisi, Cilt: 11 Sayı: 2, 103–111. https://doi.org/10.30569/adiyamansaglik.1694274

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