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Journals / Adıyaman Üniversitesi Sağlık Bilimleri Dergisi / 2026 / Cilt: 11 Sayı: 2

Disrupted antioxidant defense and oxidative stress in amyotrophic lateral sclerosis: A comprehensive biomarker analysis

Amyotrofik lateral sklerozda antioksidan savunma bozukluğu ve oksidatif stres: Kapsamlı biyomarker analizi

Zeliha Işık Bucak(ÇUKUROVA ÜNİVERSİTESİ)0009-0001-6581-4246
Derya Kocamaz(CUKUROVA UNIVERSITY)0000-0002-0705-4672
Şule Menziletoğlu Yıldız(CUKUROVA UNIVERSITY)0000-0002-6089-9834
Pages
84–91
Publication Date
2025-08-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Monitoring these biomarkers could provide valuable insights into disease mechanisms and support the development of new therapies.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aim: This study aimed to assess oxidative stress in ALS patients by measuring antioxidant enzyme activities and lipid peroxidation levels.
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Key Findings & Data:Materials and Methods: A retrospective case-control study was conducted involving 29 ALS patients and 24 healthy controls.
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Conclusion & Impact:Monitoring these biomarkers could provide valuable insights into disease mechanisms and support the development of new therapies.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Antioksidan sistem · Kan antioksidan profili · Lipid peroksidasyonu

Abstract

Aim: This study aimed to assess oxidative stress in ALS patients by measuring antioxidant enzyme activities and lipid peroxidation levels. Materials and Methods: A retrospective case-control study was conducted involving 29 ALS patients and 24 healthy controls. Blood samples were analyzed for catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione peroxidase (GPx), glutathione (GSH), and lipid peroxidation using the thiobarbituric acid reactive substances (TBARs) assay. Results: ALS patients showed significantly reduced levels of CAT, SOD, GST, and GSH, whereas GPx activity and TBARs were elevated. A negative correlation between TBARs and GST indicated impaired detoxification capacity. Principal Component Analysis demonstrated clear separation between groups, primarily driven by GPx and TBARs. Conclusion: These findings suggest a pronounced oxidative imbalance in ALS patients, marked by diminished antioxidant defenses and elevated lipid peroxidation. This imbalance may contribute to disease progression by promoting oxidative damage and motor neuron degeneration. Monitoring these biomarkers could provide valuable insights into disease mechanisms and support the development of new therapies.

Özet

Amaç: Bu çalışma, ALS hastalarında antioksidan enzim aktiviteleri ve lipit peroksidasyon düzeylerini ölçerek oksidatif stresi değerlendirmeyi amaçlamıştır. Gereç ve Yöntem: Bu retrospektif olgu-kontrol çalışmasına, 29 ALS tanılı hasta ve ve 24 sağlıklı birey dahil edilmiştir. Kan örneklerinde katalaz (CAT), süperoksit dismutaz (SOD), glutatyon S-transferaz (GST), glutatyon peroksidaz (GPx), glutatyon (GSH) ve lipit peroksidasyonu düzeyleri tiyobarbitürik asit reaktif maddeleri (TBARs) testi ile analiz edilmiştir. Bulgular: ALS hastalarında CAT, SOD, GST ve GSH düzeyleri anlamlı şekilde düşük bulunurken, GPx aktivitesi ve TBARs düzeyleri artış göstermiştir. TBARs ile GST arasında saptanan negatif korelasyon, detoksifikasyon kapasitesinin bozulduğunu göstermektedir. Temel Bileşenler Analizi, özellikle GPx ve TBARs düzeylerinin etkisiyle gruplar arasında belirgin bir ayrım ortaya koymuştur. Sonuç: Bu bulgular, ALS hastalarında belirgin bir oksidatif dengesizlik olduğunu, antioksidan savunmanın azaldığını ve lipit peroksidasyonunun arttığını göstermektedir. Bu dengesizlik, oksidatif hasarı artırarak motor nöron dejenerasyonuna ve hastalık progresyonuna katkıda bulunabilir. Bu biyobelirteçlerin izlenmesi, hastalık mekanizmalarının daha iyi anlaşılmasına ve hedefe yönelik antioksidan tedavilerin geliştirilmesine katkı sağlayabilir.

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Cite This Article

Bucak, Z. I., Kocamaz, D., Yıldız, Ş. M. (2025). Disrupted antioxidant defense and oxidative stress in amyotrophic lateral sclerosis: A comprehensive biomarker analysis. Adıyaman Üniversitesi Sağlık Bilimleri Dergisi, Cilt: 11 Sayı: 2, 84–91. https://doi.org/10.30569/adiyamansaglik.1671769

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