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Journals / Adıyaman Üniversitesi Sağlık Bilimleri Dergisi / 2025 / Cilt: 11 Sayı: 3

Chemical characterization and antiproliferative activity of bee venoms from Türkiye against NSCLC

Türkiye kökenli arı zehirlerinin kimyasal karakterizasyonu ve NSCLC’ye karşı antiproliferatif etkisi

Fatma Şengül Bağ(ADIYAMAN ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ)0000-0002-7097-6266
Hüsamettin Vatansev(SELÇUK ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-0230-3414
Pages
205–214
Publication Date
2025-12-31
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: H1, which is rich in melittin, showed strong antiproliferative activity on NSCLC cells with relatively low toxicity to normal cells, indicating its potential as a natural anticancer agent.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: This study compared the chemical composition and cytotoxic effect of bee venom samples from Adıyaman (A1), Bursa (B1) and a purified sample (H1) against A549 non-small cell lung cancer (NSCLC) cells.
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Key Findings & Data:Materials and Methods: Samples were filtered, lyophilized, and, for H1, further purified.
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Conclusion & Impact:Conclusion: H1, which is rich in melittin, showed strong antiproliferative activity on NSCLC cells with relatively low toxicity to normal cells, indicating its potential as a natural anticancer agent.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Arı zehiri · Melittin · A549 hücreleri

Abstract

Aim: This study compared the chemical composition and cytotoxic effect of bee venom samples from Adıyaman (A1), Bursa (B1) and a purified sample (H1) against A549 non-small cell lung cancer (NSCLC) cells. Materials and Methods: Samples were filtered, lyophilized, and, for H1, further purified. The levels of melittin, PLA2, and apamin were quantified by high-performance liquid chromatography (HPLC). Cytotoxicity was assessed in the A549 and HEK-293 cells using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay after 24 h of treatment, with cisplatin as a positive control. IC₅₀ values were calculated to compare the potency of each sample and assess selectivity toward cancer cells. Results: H1 had the highest melittin content (425.6 mg/L) and strongest cytotoxicity in A549 cells (IC50: 1.964 ± 0.013 µg/mL), outperforming A1, B1 and cisplatin (p<0.0001). Conclusion: H1, which is rich in melittin, showed strong antiproliferative activity on NSCLC cells with relatively low toxicity to normal cells, indicating its potential as a natural anticancer agent.

Özet

Amaç: Bu çalışmada, Adıyaman (A1) ve Bursa (B1) illerinden elde edilen arı zehiri örnekleri ile saflaştırılmış bir örnek (H1)'in kimyasal bileşimi ve sitotoksik etkileri, A549 küçük hücre dışı akciğer kanseri (NSCLC) hücreleri üzerinde karşılaştırılmıştır. Gereç ve Yöntem: Örnekler filtrelenmiş, liyofilize edilmiş ve H1 örneği için ek saflaştırma işlemi uygulanmıştır. Melittin, PLA₂ ve apamin düzeyleri yüksek performanslı sıvı kromatografi (HPLC) ile nicel olarak belirlenmiştir. Sitotoksisite, A549 ve HEK-293 hücrelerinde, 24 saatlik uygulama sonrasında, pozitif kontrol olarak sisplatin kullanılarak MTT [3-(4,5-dimetiltiazol-2-il)-2,5-difenil tetrazolyum bromür] testi ile değerlendirilmiştir. IC₅₀ değerleri, her bir örneğin etkinliğini karşılaştırmak ve kanser hücrelerine yönelik seçiciliğini değerlendirmek amacıyla hesaplanmıştır. Bulgular: H1 örneği en yüksek melittin içeriğine (425,6 mg/L) ve A549 hücrelerinde en güçlü sitotoksisiteye (IC50: 1,964 ± 0,013 µg/mL) sahip olup A1, B1 ve sisplatinden anlamlı düzeyde daha etkilidir (p<0,0001). Sonuç: Melittin açısından zengin olan H1 örneği, NSCLC hücrelerinde güçlü antiproliferatif aktivite göstermiş ve normal hücrelere karşı göreceli olarak düşük toksisite sergileyerek doğal bir antikanser ajan olma potansiyelini ortaya koymuştur.

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Cite This Article

Bağ, F. Ş., Vatansev, H. (2025). Chemical characterization and antiproliferative activity of bee venoms from Türkiye against NSCLC. Adıyaman Üniversitesi Sağlık Bilimleri Dergisi, Cilt: 11 Sayı: 3, 205–214. https://doi.org/10.30569/adiyamansaglik.1727540

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