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Journals / Adıyaman Üniversitesi Sağlık Bilimleri Dergisi / 2026 / Cilt: 12 Sayı: 2

In vitro evaluation of the antimicrobial, antioxidant, and cytotoxic effects of Zingiber officinale Roscoe ethanolic extract on A2780 ovarian cancer cells

Zingiber officinale Roscoe etanolik ekstraktının antimikrobiyal, antioksidan ve A2780 over kanseri hücre hattı üzerindeki sitotoksik etkilerinin in vitro değerlendirilmesi

Sümeyye Akyüz(Erzincan Binali Yıldırım Üniversitesi Tıp Fakültesi)0000-0003-1999-7827
Emrullah Akyüz(SAKARYA YENİKENT DEVLET HASTANESİ)0000-0002-5475-6394
Murat Günay(ERZİNCAN ÜNİVERSİTESİ)0000-0002-2079-6654
Pages
176–187
Publication Date
2026-08-20
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The originality of this study lies in the simultaneous investigation of ZRE’s multifaceted biological activities.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aim: To evaluate the in vitro antimicrobial, antioxidant, and anticancer activities of the ethanol extract obtained from Zingiber officinale Roscoe rhizomes (ZRE) against the A2780 ovarian cancer cell line.
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Key Findings & Data:Results: The highest antimicrobial activity was observed against Staphylococcus aureus (16,67±0,57 mm, MIC 64 µg/mL) and Candida albicans (18,67±0,57 mm, MIC 64 µg/mL).
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Conclusion & Impact:The originality of this study lies in the simultaneous investigation of ZRE’s multifaceted biological activities.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Zencefil · Antimikrobiyal Etkinlik · İn vitro

Abstract

Aim: To evaluate the in vitro antimicrobial, antioxidant, and anticancer activities of the ethanol extract obtained from Zingiber officinale Roscoe rhizomes (ZRE) against the A2780 ovarian cancer cell line. Materials and Methods: Antimicrobial activity was assessed using disk diffusion and broth microdilution methods. Antioxidant capacity was determined by DPPH and ABTS radical scavenging assays. Anticancer effects were investigated via the XTT cell viability assay. Results: The highest antimicrobial activity was observed against Staphylococcus aureus (16,67±0,57 mm, MIC 64 µg/mL) and Candida albicans (18,67±0,57 mm, MIC 64 µg/mL). Strong, concentration-dependent antioxidant activity was detected in both DPPH and ABTS assays. The IC₅₀ values in the A2780 cell line were determined to be 185±12 µg/mL at 24h and 92±8 µg/mL at 48h. Conclusion: ZRE exhibited notable antimicrobial activity, potent antioxidant capacity, and cytotoxic effects. The originality of this study lies in the simultaneous investigation of ZRE’s multifaceted biological activities.

Özet

Amaç: Bu çalışmada, Zingiber officinale Roscoe rizomlarından elde edilen etanol ekstraktının (ZRE) in vitro koşullarda antimikrobiyal, antioksidan ve A2780 over kanseri hücre hattı üzerindeki antikanser etkinliklerinin değerlendirilmesi amaçlandı. Gereç ve Yöntem: Antimikrobiyal etkinlik disk difüzyon ve sıvı mikrodilüsyon yöntemleri ile değerlendirildi. Antioksidan kapasite DPPH ve ABTS radikal giderme testleri ile belirlendi. Antikanser etki XTT hücre canlılığı testi ile incelendi. Bulgular: En yüksek antimikrobiyal etki Staphylococcus aureus (16,67±0,57 mm, 64 µg/mL) ve Candida albicans (18,67±0,57 mm, 64 µg/mL) suşlarında belirlendi. DPPH ve ABTS testlerinde güçlü ve konsantrasyona bağlı antioksidan aktivite gözlendi. A2780 hücre hattında IC₅₀ değerleri 24 ve 48 saat sonunda sırasıyla 185±12 µg/mL ve 92±8 µg/mL olarak hesaplandı. Sonuç: ZRE’nin belirgin antimikrobiyal aktiviteye, güçlü antioksidan kapasiteye ve sitotoksik etkiye sahip olduğu belirlendi. Çalışmanın özgün yanı, ZRE’nin çok yönlü biyolojik aktivitelerinin birlikte incelenmesidir.

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Cite This Article

Akyüz, S., Akyüz, E., Günay, M. (2026). In vitro evaluation of the antimicrobial, antioxidant, and cytotoxic effects of Zingiber officinale Roscoe ethanolic extract on A2780 ovarian cancer cells. Adıyaman Üniversitesi Sağlık Bilimleri Dergisi, Cilt: 12 Sayı: 2, 176–187. https://doi.org/10.30569/adiyamansaglik.1858186

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