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Journals / Türkiye Biyolojik Mücadele Dergisi / 2026 / Cilt: 17 Sayı: 2026

In vitro biocontrol potential of coelomic fluid from the red Californian earthworm against Alternaria solani

Kızıl Kaliforniya solucanı sölom sıvısının Alternaria solani’ye karşı in vitro biyokontrol potansiyeli

Nil Melisa Yılmaz(AKDENİZ ÜNİVERSİTESİ)0009-0007-6851-9182
Ozer Calıs(AKDENİZ ÜNİVERSİTESİ)0000-0002-7219-1219
Pages
1–15
Publication Date
2026-09-29
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The findings revealed that endogenous Bacillus sp. and secondary microbiota naturally present in the crude coelomic matrix colonized the unfiltered culture media, demonstrating that sterile filtration is essential to accurately evaluate the direct coelomolytic humoral effect.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, the in vitro antagonist and biocontrol potential of coelomic fluid extracted from the Red Californian earthworm (Eisenia fetida) via electro-stimulation was investigated against Alternaria solani, an important phytopathogen causing early blight in tomatoes.
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Key Findings & Data:To determine the methodological effects on the bioactivity of the coelomic fluid, Single-Centrifuged (TS) and Double-Centrifuged (CS) formulations, along with Crude and Sterile-Filtered (0.22 μm) applications, were compared using dual-culture assays.
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Conclusion & Impact:The findings revealed that endogenous Bacillus sp. and secondary microbiota naturally present in the crude coelomic matrix colonized the unfiltered culture media, demonstrating that sterile filtration is essential to accurately evaluate the direct coelomolytic humoral effect.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Antifungal aktivite · Eisenia fetida · inhibisyon zonu

Abstract

In this study, the in vitro antagonist and biocontrol potential of coelomic fluid extracted from the Red Californian earthworm (Eisenia fetida) via electro-stimulation was investigated against Alternaria solani, an important phytopathogen causing early blight in tomatoes. To determine the methodological effects on the bioactivity of the coelomic fluid, Single-Centrifuged (TS) and Double-Centrifuged (CS) formulations, along with Crude and Sterile-Filtered (0.22 μm) applications, were compared using dual-culture assays. The findings revealed that endogenous Bacillus sp. and secondary microbiota naturally present in the crude coelomic matrix colonized the unfiltered culture media, demonstrating that sterile filtration is essential to accurately evaluate the direct coelomolytic humoral effect. In sterile-filtered coelomic fluid assays, while the inhibition zone in the control group was almost completely closed (0.50 mm) on the 18th day of incubation, the Single-Centrifuged (TS) sterile coelomic fluid created a strong and stable inhibition zone of 24.34 mm, highly suppressing fungal mycelial growth. Conversely, in the Double-Centrifuged (CS) group, the inhibition zone dropped to 6.81 mm. In conclusion, sterilized Single-Centrifuged (TS) E. fetida coelomic fluid shows strong potential as an eco-friendly and sustainable biopesticide component for controlling A. solani.

Özet

Bu çalışmada, Kızıl Kaliforniya solucanından (Eisenia fetida) elektro-stimülasyon yöntemiyle elde edilen sölom sıvısının, domateste erken yanıklık hastalığına neden olan önemli fitopatojen Alternaria solani üzerindeki in vitro antagonist ve biyokontrol potansiyeli araştırılmıştır. Sölom sıvısının biyoaktivitesi üzerindeki metodolojik etkileri belirlemek amacıyla Tek Santrifüjlü (TS) ve Çift Santrifüjlü (ÇS) formülasyonlar ile Ham ve Steril Filtreli (0.22 µm) uygulamalar ikili kültür testlerinde karşılaştırılmıştır. Elde edilen bulgular, filtresiz ham sölom sıvısı ortamında matrisin doğasından kaynaklanan endojen Bacillus sp. ve sekonder mikrobiyota gelişiminin gözlendiğini; doğrudan sölomolitik hümoral etkinin net olarak değerlendirilebilmesi için steril filtrasyonun zorunlu olduğunu göstermiştir. Steril filtre edilmiş sölom sıvısı testlerinde, inkübasyonun 18. gününde kontrol grubunda inhibisyon bölgesi tamamen kapanırken (0.50 mm), Tek Santrifüjlü (TS) steril sölom sıvısı 24.34 mm'lik güçlü ve kararlı bir inhibisyon zonu oluşturarak fungal misel gelişimini yüksek oranda baskılamıştır. Buna karşın Çift Santrifüjlü (ÇS) grupta inhibisyon zonu 6.81 mm'ye gerilemiştir. Sonuç olarak, sterilize edilmiş Tek Santrifüjlü (TS) E. fetida sölom sıvısının, A. solani kontrolünde çevreci ve sürdürülebilir bir biyopestisit bileşeni olarak güçlü bir potansiyel taşıdığı ortaya konmuştur.

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Cite This Article

Yılmaz, N. M., Calıs, O. (2026). In vitro biocontrol potential of coelomic fluid from the red Californian earthworm against Alternaria solani. Türkiye Biyolojik Mücadele Dergisi, Cilt: 17 Sayı: 2026, 1–15. https://doi.org/10.31019/tbmd.2003648

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