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Journals / Archives of Clinical and Experimental Medicine / 2019 / Cilt: 4 - Sayı: 2

Thymoquinone exhibits anti-inflammatory, antioxidant, and immunomodulatory effects on allergic airway inflammation

Timokinon allerjik solunum yolu inflamasyonu üzerine anti-inflamatuar, antioksidan ve immünomodülatör etkiler gösterir

ALİ PARLAR
SEYFULLAH OKTAY ARSLAN
Pages
60–65
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: These findings reveal that TQ could be beneficial in asthma pathophysiology due to its immunomodulatory, anti-inflammatory, and antioxidant effects.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: Asthma is an allergic disease causing mucus secretion, release various pro-inflammatory mediators such as tumor necrosis factor- α (TNF-α) and interleukins.
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Key Findings & Data:Inflammatory cells, interleukin (IL)-6 and TNF-α in bronchoalveolar lavage (BAL) fluid, and lipid peroxidation (LPO) in lung tissue were measured.
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Conclusion & Impact:Conclusion: These findings reveal that TQ could be beneficial in asthma pathophysiology due to its immunomodulatory, anti-inflammatory, and antioxidant effects.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:TNF-α · Alerjik solunum yolu enflamasyonu · mikrovasküler sızıntı

Abstract

Aim: Asthma is an allergic disease causing mucus secretion, release various pro-inflammatory mediators such as tumor necrosis factor- α (TNF-α) and interleukins. The aim of this study was to evaluate the effect of thymoquinone (TQ) on allergic airway inflammation in rats. Methods: Allergic airway inflammation induced by ovalbumin (OVA) challenge in sensitized-rats and effect of TQ were studied. Inflammatory cells, interleukin (IL)-6 and TNF-α in bronchoalveolar lavage (BAL) fluid, and lipid peroxidation (LPO) in lung tissue were measured. Microvascular leakage was detected by Evans blue dye leakage in airway tissues. Results: Tidal volume was significantly lower in OVA group (1.4± 0.07 ml) than control group (1.9±0.04 ml) (p = 0.002), while breathing frequency was significantly higher in OVA group (135.3±12.9 min-1) than control group (p=0.017). In terms of tidal volume, statistical significance between TQ30 and OVA groups was found (1.8±0.07 ml) (p=0.008), while in terms of breathing frequency, no significance was found between both of them (126.7±7.3). Total white blood cell count was significantly higher in OVA group (1,376.8±136.4 x103/ml) than control group (545.0±106.7 x103/ml) (p<0.001). Statistical significance was found in TQ10 (824.7±4.5 x103/ml) group when compared OVA group (p=0.036), while statistical significance was not found in TQ1 group (1,282.2±137.7 x103/ml). When compared OVA group (60.3±4.9 pg/ml) with control group in terms of the TNF-α level, statistical significance was found (36.7± 4.7 pg/ml) (p=0.011). The Evans blue dye level was significantly higher in OVA group (31.8±3.6 ng/mg of tissue) than control (12.5±1.1 ng/mg of tissue) group (p<0.001), and TQ10 group (16.3±6.7 ng/mg of tissue) (p=0.002), and TQ30 (13.5±1.0 ng/mg of tissue) group (p<0.001). Conclusion: These findings reveal that TQ could be beneficial in asthma pathophysiology due to its immunomodulatory, anti-inflammatory, and antioxidant effects.

Özet

Amaç: Astım, mukus sekresyonuna neden olan, TNF-α, IL'ler gibi çeşitli proinflamatuar mediatörleri salgılayan alerjik bir hastalıktır. Timokinon’un (TQ) sıçanlarda alerjik solunum yolu inflamasyonu üzerindeki etkisini değerlendirmektir. Yöntemler: Duyarlılaştırılmış sıçanlarda ovalbumin (OVA) ile indüklen alerjik solunum yolu enflamasyonu ve TQ etkisi çalışıldı. Bronkoalveoler lavaj (BAL) sıvısında enflamatuar hücre sayıları, interlökin (IL)-6 ve tümör nekroz faktör alfa (TNF-α) düzeyleri ve akciğer dokusunda lipit peroksidasyonu (LPO) seviyeleri ölçüldü. Mikrovasküler sızıntı, solunum yolu dokusunda Evans mavisi ile tespit edildi. Bulgular: Bu çalışmada, OVA grubunda tidal hacim (1,4±0,07 ml), kontrol grubuna göre önemli ölçüde düşük bulunmuştur (1,9±0,04 ml) (p = 0,002). OVA grubunda (135,3±12,9 dk-1) solunum sıklığı kontrol grubuna (p=0,017) göre anlamlı bulunmuştur. Tidal hacim açısından TQ30 (1,8±0,07 ml) grubu ile OVA grubu karşılaştırıldığında istatistiksel anlamlılık bulunurken (p=0,008), solunum frekansı açısından gruplar arasında istatistiksel anlamlılık bulunamamıştır (126,7±7,3 dk-1). OVA grubunda (1.376,8±136,4 x103/ml) toplam beyaz küre sayısı, kontrol grubuna göre anlamlıydı (545,0±106,7 x103/ml) (p<0,001). TQ10 (824,7±4,5 x103/ml) grubu ile OVA grubu arasında istatistiksel anlamlılık bulunurken (p=0,036), TQ1 (1.282,2±137,7 x103/ml) ile OVA grubu arasında istatistiksel anlamlılık yoktu. TNF-α yönünden OVA grubu (60,3±4,9 pg/ml) ile kontrol grubu (36,7±4,7 pg/ml) karşılaştırıldığında, aralarında istatistiksel anlamlılık bulundu (p=0,011). OVA grubunda Evans mavi düzeyi (31,8±3,6 ng/mg doku), kontrol (12,5±1,1 ng/mg doku) (p<0,001), TQ10 (16,3±6,7 ng/mg doku) (p=0,002) ve TQ30 (13,5±1,0 ng/mg doku) (p<0,001) gruplarına göre önemli derecede yüksekti. Sonuç: Bu bulgular, timokinonun immünomodülatör, antienflamatuar ve antioksidan etkileri nedeniyle astım patofizyolojisinde yararlı olabileceğini ortaya koymaktadır.

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Cite This Article

PARLAR, A., ARSLAN, S. O. (2019). Thymoquinone exhibits anti-inflammatory, antioxidant, and immunomodulatory effects on allergic airway inflammation. Archives of Clinical and Experimental Medicine, Cilt: 4 - Sayı: 2, 60–65. https://ajindex.com/articles/142494

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