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Journals / International Journal of Secondary Metabolite / 2026 / Volume: 6 Issue: 1

Determination of anticancer effects of Urospermum picroides against human cancer cell lines

Mehlika Alper(MUGLA SITKI KOCMAN UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF MOLECULAR BİOLOGY and GENETICS)0000-0001-6193-346X
Hatice Güneş(MUGLA SITKI KOCMAN UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF BİOLOGY)0000-0001-5191-365X
Pages
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Publication Date
2019-03-16
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These findings suggest that the flowering parts of U. picroides may be a potential source for anticancer agents.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Plants continue to be a good source for developing effective anticancer agents.
📊
Key Findings & Data:Daudi, A549 and HeLa cancer cell lines and BEAS-2B normal cell line were used to identify the cytotoxic effect of the extract using MTT assay.
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Conclusion & Impact:These findings suggest that the flowering parts of U. picroides may be a potential source for anticancer agents.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Urospermum picroides · cytotoxicity · cell cycle analysis

Abstract

Plants continue to be a good source for developing effective anticancer agents. In this study, in vitro various biological effects of crude ethanolic extract from flowering parts of Urospermum picroides collected from the Muğla province of Turkey were investigated for the first time . Daudi, A549 and HeLa cancer cell lines and BEAS-2B normal cell line were used to identify the cytotoxic effect of the extract using MTT assay. The effect of the extract on cell cycle progression was detected by flow cytometric analysis. The level of VEGF, IL-1α, IL-6 and TNF-α secretion in the cells treated with the extract were measured using ELISA The extract caused a higher cytotoxic effect on Daudi cells with an IC 50 value of 85.64 µg/mL than the other cells tested. The IC 50 values in HeLa and A549 cells were determined to be 135.35 and 234.8 µg/ mL , respectively. The selective cytotoxicity was considered between Daudi and BEAS-2B (109.80 µg/ mL ) cell lines. In addition, the effect of the extract on cell cycle progression changes according to cell line used. Moreover, the extract decreased the level of secreted VEGF in treated A549 cells by 31%. In addition, the extract resulted in a significant decrease in the secretion of IL-1α, IL-6 and TNF-α cytokines in A549 and Daudi cells compared to the untreated cells. These findings suggest that the flowering parts of U. picroides may be a potential source for anticancer agents.

Özet

Plants continue to be a good source for developing effective anticancer agents. In this study, in vitro various biological effects of crude ethanolic extract from flowering parts of Urospermum picroides collected from the Muğla province of Turkey were investigated for the first time. Daudi, A549 and HeLa cancer cell lines and BEAS-2B normal cell line were used to identify the cytotoxic effect of the extract using MTT assay. The effect of the extract on cell cycle progression was detected by flow cytometric analysis. The level of VEGF, IL-1α, IL-6 and TNF-α secretion in the cells treated with the extract were measured using ELISA The extract caused a higher cytotoxic effect on Daudi cells with an IC50 value of 85.64 µg/mL than the other cells tested. The IC50 values in HeLa and A549 cells were determined to be 135.35 and 234.8 µg/ mL, respectively. The selective cytotoxicity was considered between Daudi and BEAS-2B (109.80 µg/mL) cell lines. In addition, the effect of the extract on cell cycle progression changes according to cell line used. Moreover, the extract decreased the level of secreted VEGF in treated A549 cells by 31%. In addition, the extract resulted in a significant decrease in the secretion of IL-1α, IL-6 and TNF-α cytokines in A549 and Daudi cells compared to the untreated cells. These findings suggest that the flowering parts of U. picroides may be a potential source for anticancer agents.

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Cite This Article

Alper, M., Güneş, H. (2019). Determination of anticancer effects of Urospermum picroides against human cancer cell lines. International Journal of Secondary Metabolite, Volume: 6 Issue: 1. https://doi.org/10.21448/ijsm.482404

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