SavedAdd your journal

Journals / International Journal of Secondary Metabolite / 2020 / Volume: 7 Issue: 4

Investigation of Some Biological Activities of Extracts Centranthus longiflorus subsp. longiflorus

Elif Ayar(AMASYA UNIVERSITY)0000-0001-9281-1545
Nezahat Kandemir(FACULTY OF EDUCATION)0000-0002-5428-4139
Dr. Şevket Kandemir(FACULTY OF EDUCATION)0000-0001-6781-0057
Umut Çelikoğlu(AMASYA UNIVERSITY, FACULTY OF SCIENCE AND LETTERS)0000-0003-0995-8154
Önder İdil(FACULTY OF EDUCATION)0000-0003-1744-4006
Pages
—
Publication Date
2020-12-15
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to the data obtained, especially the below-ground hexane (MIC value:375μg/mL) extract showed more antimicrobial activity than other plant extracts, and it was found to be more effective Gram negative bacteria.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, the effects of above and below-ground extracts of Centranthus longiflorus subsp. longiflorus plant, commonly found in Turkey, on antioxidant, antimicrobial and DNA damage were evaluated.
📊
Key Findings & Data:Additionally, the effects of plant extracts on DNA damage were investigated using pBR322 plasmid DNA.
💡
Conclusion & Impact:According to the data obtained, especially the below-ground hexane (MIC value:375μg/mL) extract showed more antimicrobial activity than other plant extracts, and it was found to be more effective Gram negative bacteria.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Centranthus longiflorus · Antimicrobial · Antioxidant

Abstract

In this study, the effects of above and below-ground extracts of Centranthus longiflorus subsp. longiflorus plant, commonly found in Turkey, on antioxidant, antimicrobial and DNA damage were evaluated. Plant extracts were prepared by applying three different solvents (hexane, methanol and ethanol). The antimicrobial activity tests of the extracts were performed using four different standard strains and one yeast. DPPH, total phenolic content calculation and CUPRAC methods were applied for antioxidant activity studies. Additionally, the effects of plant extracts on DNA damage were investigated using pBR322 plasmid DNA. According to the data obtained, especially the below-ground hexane (MIC value:375μg/mL) extract showed more antimicrobial activity than other plant extracts, and it was found to be more effective Gram negative bacteria. The highest antioxidant activity was determined in extracts prepared with above (IC50 value of methanol extract:4.5mg/mL) and below-ground (IC50 value of methanol extract:5.7mg/mL) methanol. The above (93,9 μg GAE/mL) and below-ground (96.9 μg GAE/mL) methanol extracts were seen to have high total phenolic content. It has also been observed that above-ground hexane and methanol extracts have no effect on pBR322 plasmid DNA, but other extracts affect pBR322 plasmid DNA in the direction of degradation or deformation. Especially, the extracts of the above and below-ground ethanol had the effect of completely eliminating the open ring form. Therefore, it was concluded that this taxon could be widely used in the treatment and prevention of oxidative stress-related diseases in the future.

Özet

In this study, the effects of above and below-ground extracts of Centranthus longiflorus subsp. longiflorus plant, commonly found in Turkey, on antioxidant, antimicrobial and DNA damage were evaluated. Plant extracts were prepared by applying three different solvents (hexane, methanol and ethanol). The antimicrobial activity tests of the extracts were performed using four different standard strains and one yeast. DPPH, total phenolic content calculation and CUPRAC methods were applied for antioxidant activity studies. Additionally, the effects of plant extracts on DNA damage were investigated using pBR322 plasmid DNA. According to the data obtained, especially the below-ground hexane (MIC value:375μg/mL) extract showed more antimicrobial activity than other plant extracts, and it was found to be more effective Gram negative bacteria. The highest antioxidant activity was determined in extracts prepared with above (IC50 value of methanol extract:4.5mg/mL) and below-ground (IC50 value of methanol extract:5.7mg/mL) methanol. The above (93,9 μg GAE/mL) and below-ground (96.9 μg GAE/mL) methanol extracts were seen to have high total phenolic content. It has also been observed that above-ground hexane and methanol extracts have no effect on pBR322 plasmid DNA, but other extracts affect pBR322 plasmid DNA in the direction of degradation or deformation. Especially, the extracts of the above and below-ground ethanol had the effect of completely eliminating the open ring form. Therefore, it was concluded that this taxon could be widely used in the treatment and prevention of oxidative stress-related diseases in the future.

🔖

Cite This Article

Ayar, E., Kandemir, N., Kandemir, D. Ş., Çelikoğlu, U., İdil, Ö. (2020). Investigation of Some Biological Activities of Extracts Centranthus longiflorus subsp. longiflorus. International Journal of Secondary Metabolite, Volume: 7 Issue: 4. https://doi.org/10.21448/ijsm.648072

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (46)

  1. Güner, A. (2012). Centranthus DC. In List of Turkey Plants (Vascular Pants), ed.; Aslan, S., Ekim, T., Vural, M., Babaç, M.T.; Publication of Nezahat Gökyiğit Botanic Garden and Floristic Research Society: İstanbul, Turkey, pp. 314. ISSN:978-605-60425-7-7.
  2. Baytop, T. (1999). Used Medicine Plants in Turkey. In Therapy with Plant in Turkey (Past and Present). Ed.; Baytop, T.; Nobel Medicine Bookstores: İstanbul, Turkey, 975-420-021-1.
  3. Rice-Evans, C., Miller, A., Paganga, G. (1997). Antioxidant Properties of Phenolic Compounds. Trends Plant Sci., 2, 152-59. https://doi.org/10.1016/SI3601385(97)01018-2
  4. Rammal, H., Farhan, H., Hijazi, A., Bassal, A., Kobeissy, A., Badrtan, B. (2013). Phytochemical Screening and Antioxidant Activity of Centranthus longiflorus L. J. Nat Prod Plant Resour., 3, 29-36.
  5. Wong, C.C., Li, H.B., Cheng, K.W., Chen, F. (2006). A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chem., 97, 705-711. https://doi.org/10.1016/j.foodchem.2005.05.049
  6. Sener, B., Mutlugil, A., Bingöl, F. (2008). Analysis of valepotriates in Centranthus longiflorus Stev. by reversed-phase high pressure liquid chromatography. Pharm Biol., 25, 133-136. https://doi.org/10.3109/13880208709060916
  7. Demirezer, L.Ö., Güvenalp, Z., Schiewe, H.J., Strietzel, I., Harmandar, M., Zeeck, A. (1999). Iridoids from Centranthus longiflorus ssp. longiflorus. Phytochemistry, 51, 909-912. https://doi.org/10.1016/s0031-9422(99)00152-1
  8. Kuruüzüm, A-Uz., Güvenalp, Z., Demirezer, L.Ö., Bergere, I., Stroch, K., Zeeck, A. (2002). 4-deoxy iridoid glycoides from Centranthus longiflorus. Phytochemistry, 61, 937-941. https://doi.org/10.1016/S0031-9422(02)00476-4
  9. Hassan, E., Tayebeh, R., Samaneh, E.T. (2008). Quantification of Valerenic Acid and its derivatives in some species of Valeriana L. and Centranthus longiflorus Stev. Asian J. Plant Sci., 7, 195-200. https://doi.org/10.3923/ajps.2008.195.200
  10. Makki, R., Dirani, Z. E., Rammal, H., Sweidan, A., Al Bazzal, A., Chokr, A. (2015). Antibacterial Activity of Two Lebanese Plants: Eryngium creticum and Centranthus longiflorus. J. Nanomed. Nanotechnol., 6, 315-320. https://doi.org/10.4172/2157-7439.1000315
  11. Aliyazıcıoğlu, R., Korkmaz, N., Akkaya, S., Sener, S.Ö., Badem, M., Karaoğlu, S.A., Eyüpoğlu, O.E. (2016). Phenolic components, antioxidant and antimicrobial activities of Centranthus longiflorus L. Int. J. Adv. Res. Biol. Sci., 3, 80-87. https://doi.org/10.22192/ijarbs.2016.03.10.012
  12. Cowan, M.M. (1999). Plant Products as Antimicrobial Agent. Clin microbiol Rev., 12, 564-582. https://doi.org/10.1128/CMR.12.4.564
  13. Okunade, A.L., Elvin-Lewis, M.P.F. (2004). Natural Antimycobacterial Metabolites: Current Status. Phytochemistry, 65, 1017-1032. https://doi.org/10.1016/j.phytochem.2004.02.013
  14. Taguri, T., Tanaka, T., Kouno, I. (2004). Antimicrobial Activity of 10 Different Plant Polyphenol Against Bacteria Causing Food-Borne Disease. Biol Pharm Bull., 27, 1965-1969. https://doi.org/10.1248/bpb.27.1965
  15. Cheesman, L., Nair, J.J., Van Staden, J. (2012). Antibacterial Activity of Crinane Alkaloids From Boophone disticha (Amaryllidaceae). J Ethnopharmacol., 140, 405-408. https://doi.org/10.1016/j.jep.2012.01.037
  16. Topal, Y. (2013). Investigation of Antioxidant and Antimicrobial Effects of Phenolic Compounds of Some Species of Alchemilla L. (Rosaceae) genus. Master thesis, Bingöl Üniversity, Institute of Science and Technology, Bingöl, 2013.
  17. Kawanishi, S., Hiraku, Y. (2004). Amplification of anticancer drug-induced DNA damage and apoptosis by DNA-binding compounds. Curr Med Chem-Anticancer Agents, 4(5), 415-419. https://doi.org/10.2174/1568011043352867
  18. Richardson, I.B.K. (1972). Centranthus DC. In Flora of Turkey and The East Aegean Islands, 4th ed.; Davis, H.P., Chamberlain, D.F., Phil, D., Victoria, A., Matthews, B.; Edinburgh University Press: Edinburg, England, 1972; Volume 4, pp. 558-559; ISSN:0852242085
  19. Mammadov, R., Ili, P., Vaizoğullar, H.E., Makasçı, AA. (2011). Antioxidant Activity and Total Phenolic Content of Gagea fibrosa and Romulea ramiflora. Iran J. Chem. Chem. Eng., 30, 57-62.
  20. Singleton, V.L., Rossi, J.A. (1965). Colimetry of total phenolics with phosphomolybdic- phosphotungstic acid reagents. Am J Enol Vitic., 16, 144-158.
  21. Brand-Williams, W., Cuvelier, M.E., Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol., 28, 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
  22. Apak, R., Güçlü, K., Özyürek, M., Karademir, S.E. (2004). A novel total antioxidant capacity index for dietary polyphenols, Vitamin C and E, using their cupric ion reducing capability in the presence of neocuroine: CUPRAC method. J. Agric. Food Chem., 52, 7970- 7981. https://doi.org/10.1021/jf048741x
  23. Andrews, J.M. (2001). Determination of minimum inhibitory concentrations. J Antimicrob Chemother., 48, 5-16. https://doi.org/10.1093/jac/48.suppl._1.5
  24. Babu, J., Pramod, W.R., George, T., Nitisha, S. (2007). Standard Review Cold-active microbial Lipases: a versatile tool for industrial applications. Biotechnol. Mol. Biol. Rev., 2, 39-48.
  25. Wink, M., Ashour, M.L., El-Readi, M.Z. (2012). Secondary Metabolites from Plants Inhibiting ABC Transporters and Reversing Resistance of Cancer Cells and Microbes to Cytotoxicand Antimicrobial Agents. Front Microbiol., 3, 130. https://doi.org/10.3389/fmicb.2012.00130
  26. Law, P.C., Auyeung, K.K., Chan, L.Y., Ko, J.K. (2012). Astragalus Saponins Downregulate Vascular Endothelial Growth Factor Under Cobalt Chloride-Stimulated Hypoxia in Colon Cancer Cells. BMC Complement Altern Med., 12, 160. https://doi.org/10.1186/1472-6882-12-160
  27. Zamora-Ros, R., Agudo, A., Luján-Barroso, L., Romieu, I., Ferrari, P., Knaze, V., Sánchez-Cantalejo, E., et al. (2012). Dietary Flavonoid and Lignan Intake and Gastric Adenocarcinoma Risk in The European Prospective Investigation into Cancer and Nutrition (EPIC) study. Am J Clin Nutr., 96, 1398-1408. https://doi.org/10.3945/ajcn.112.037358
  28. Süleyman, H., Güvenalp, Z., Kızılkaya, M., Demirezer, Ö.L. (2007). Sedative effect of Centranthus longiflorus ssp. longiflorus in rats and the influence of adrenalectomy on its effect. Yakugaku Zasshi., 127, 1263-1265. https://doi.org/10.1248/yakushi.127.1263.
  29. Hassan, R., Hussein, F., Akram, H., Ali, B., Ahmed, K., Bassam, B. (2013). Phytochemical Screening and Antioxidant Activity of Centranthus longiflorus. L. J. Nat. Prod. Plant Resour., 3, 29-36.
  30. Yen, G.C., Wu, S.C., Duh, P.D. (1996). Extraction and Identification of Antioxidant Components from The Leaves of Mulberry (Morus alba L.). J. Agric. Food Chem., 44, 1687-1690. https://doi.org/10.1021/jf9503725
  31. Yılmaz, A., Yıldız, S., Tabbouche, S., Kılıç, A.O., Can, Z. (2016) Total Phenolic Content, Antioxidant and Antimicrobial Properties of Pleurotos ostreatus Grown on Lime (Tilia tomentosa) Leaves. Hacettepe J. Bio and Chem, 44, 119-124. https://doi.org/10.15671/HJBC.20184417585
  32. Zengin, G., Nithiyanantham, S., Locatelli, M., Ceylan, R., Uysal, S., Aktumsek, A., Palaniswamy, K., Maskovic, P. (2016). Screening of In vitro Antioxidant and Enzyme Inhibitory Activities of Different Extracts from Two Uninvestigated Wild Plants: Centranthus longiflorus subsp. longiflorus and Cerinthe minor subsp. auriculata. Eur. J. Integr., 8, 286-292. https://doi.org/10.1016/j.eujim.2015.12.004
  33. Turan, M., Mammadov, R. (2018). Antioxidant, Antimicrobial, Cytotoxic, Larvicidal and Anthelmintic Activities and Phenolic Contents of Cyclamen alpinum. Pharmacol Pharm., 9, 100-116. https://doi.org/10.4236/pp.2018.94008
  34. Molyneux, P. (2004). The use of the stable free radical diphenylpicryllhydrazyl (dpph) for estimating antioxidant activity. Songklanakarin J. Sci Technol., 26, 211-219.
  35. Balunas, M., Douglaskinghorn, AD. (2005). Drug discovery from medicinal plants. Life Sci., 78, 431-441. https://doi.org/10.1016/j.lfs.2005.09.012
  36. Çoban, T., Saltan, ÇG., Sever, B., İşcan, M. (2003). Antioxidant activities of plants used inTraditional Medicine in Turkey. Pharm. Biol. 41, 608-613. https://doi.org/10.1080/13880200390501974
  37. Hasanaki, Y., Ogawar, S., Fukui, S. (1994). The correlation between active oxygen species and antioxidative effects of flavonoids. Free Radic Biol Med., 16, 845-850. https://doi.org/10.1016/0891-5849(94)90202-x
  38. Sanz, MJ., Ferrandiz, M., Cejudo, M., Terencio, MC., Gil, B., Bustos, G., Ubeda, A., Gunasegara, R., Alcanaz, MJ. (1994). Influence of a series of natural flavonoids on free-radical generating systems and oxidative stress. Xenobiotica, 24, 689-699. https://doi.org/10.3109/00498259409043270
  39. Sağlam, G., Kandemir, N. (2020). Comparison of Biological and Antioxidant Activities of Above and Below-Ground Extracts of Endemic Heliotropium samolifolium subsp. erzurumicum. KSU J. Agric. Nat., 23, 1054-1063. https://doi.org/10.18016/ksutarimdoğa.vi.672571
  40. Emaduldeen, A. (2014). Investigation of Biological Activities of Iris kirkwoodii. Master Thesis, Gaziantep University, Institute of Science and Technology, Gaziantep, 2014.
  41. Azmaz, M., Aksoy, Ö.K., Katılmış, Y., Mammadov, R. (2020). Investigation of the Antioxidant Activity and Phenolic Compounds of Andricus quercustozae Gall and Host Plant (Quercus infectoria). Int. J. Second. Metab., 7, 77-87. https://doi.org/10.21448/ijsm.674930
  42. Makki, R., Rammal, H., Farhan, H., Nasser, M., Dirani, El Z., Hijazi, A. (2015). The antioxidant and anti-tumor activity of the Lebanese Centranthus longiflorus L. World J. Pharm. Sci., 3, 347-354.
  43. Büyükokuroğlu, M.E., Demirezer, L.O., Güvenalp, Z. (2002). Sedative, Anticonvulsant and Behaviour Modifying Effects of Centranthus longiflorus ssp. longiflorus: A Study of Comparison to Diazepam. Pharmazie, 57, 559-561.
  44. Orhan, E. (2015). Investigation of Biological Activities of Endemic Iris galatica. Master Thesis, Gaziantep University, Institute of Science and Technology, Gaziantep, 2015.
  45. Hundur, Ö.D., İdil, Ö., Kandemir, N., Gül, M., Konar, V. (2018). Phytochemical Screening and In vitro Antioxidant, Antimicrobial Activity and DNA Interaction of Leucojum aestivum. Fresen Environ Bull., 27, 6704-6710.
  46. Gül, M., Öztürk, Çalı, I., Cansaran, A., Idil, O., Kulu, I., Çelikoglu, U. (2017). Evaluation of Phytochemical Content, Antioxidant, Antimicrobial Activity and DNA Cleavage Effect of Endemic Linaria corifolia Desf. (Plantaginaceae). Cogent Chem., 3, 1-14. https://doi.org/10.1080/23312009.2017.1337293