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Journals / International Journal of Secondary Metabolite / 2026 / Volume: 9 Issue: 2

Effect of culture time on secondary metabolite production from MeJA-stimulated in vitro roots of Hyoscyamus niger L.

Hikmet Deveci(ISPARTA UYGULAMALI BİLİMLER ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, TARIMSAL BİYOTEKNOLOJİ BÖLÜMÜ)0000-0003-4393-8552
Tunhan Demirci(Süleyman Demirel Üniversitesi Eczacılık Fakültesi)0000-0001-8516-911X
Nilgün Göktürk Baydar(ISPARTA UYGULAMALI BİLİMLER ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, TARIMSAL BİYOTEKNOLOJİ BÖLÜMÜ)0000-0002-5482-350X
Pages
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Publication Date
2022-06-15
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was found that the fresh and dry weights and the growth index of the roots harvested between the 12th and 30th day were the highest.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study was conducted to determine the effects of different culture times on root growth and accumulation of secondary metabolites in adventitious roots from Hyoscyamus niger petioles.
📊
Key Findings & Data:For this purpose, adventitious roots transferred to culture media containing 1 mM methyl jasmonate (MeJA) were harvested at 11 different culture times, starting from day 1 to day 30.
💡
Conclusion & Impact:It was found that the fresh and dry weights and the growth index of the roots harvested between the 12th and 30th day were the highest.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Hyoscyamus niger · methyl jasmonate · adventitious roots

Abstract

This study was conducted to determine the effects of different culture times on root growth and accumulation of secondary metabolites in adventitious roots from Hyoscyamus niger petioles. For this purpose, adventitious roots transferred to culture media containing 1 mM methyl jasmonate (MeJA) were harvested at 11 different culture times, starting from day 1 to day 30. After harvest, the fresh and dry weights of the roots and the root growth index as parameters of root growth, as well as the amounts of tropane alkaloids and phenolic compounds, were examined. It was found that the fresh and dry weights and the growth index of the roots harvested between the 12th and 30th day were the highest. The highest amounts of scopolamine were obtained from roots harvested on days 15, 18, and 21. The highest hyoscyamine accumulation was found on days 12 and 15. The highest total phenolic content was 19.33 mg g-1 in root cultures harvested on day 15. HPLC analyses revealed that ferulic acid, caffeic acid, o-coumaric acid, p-coumaric acid, rutin, and quercetin were not detected in the roots. On the other hand, the amounts of gallic acid, catechin, epicatechin, vanillin, cinnamic acid, rosmarinic acid, and chlorogenic acid showed significant changes depending on the culture times. Considering all the results, it was found that the optimum culture time for the production of secondary metabolites in H. niger adventitious root cultures stimulated with 1mM MeJA was 15 days.

Özet

This study was conducted to determine the effects of different culture times on root growth and accumulation of secondary metabolites in adventitious roots from Hyoscyamus niger petioles. For this purpose, adventitious roots transferred to culture media containing 1 mM methyl jasmonate (MeJA) were harvested at 11 different culture times, starting from day 1 to day 30. After harvest, the fresh and dry weights of the roots and the root growth index as parameters of root growth, as well as the amounts of tropane alkaloids and phenolic compounds, were examined. It was found that the fresh and dry weights and the growth index of the roots harvested between the 12th and 30th day were the highest. The highest amounts of scopolamine were obtained from roots harvested on days 15, 18, and 21. The highest hyoscyamine accumulation was found on days 12 and 15. The highest total phenolic content was 19.33 mg g-1 in root cultures harvested on day 15. HPLC analyses revealed that ferulic acid, caffeic acid, o-coumaric acid, p-coumaric acid, rutin, and quercetin were not detected in the roots. On the other hand, the amounts of gallic acid, catechin, epicatechin, vanillin, cinnamic acid, rosmarinic acid, and chlorogenic acid showed significant changes depending on the culture times. Considering all the results, it was found that the optimum culture time for the production of secondary metabolites in H. niger adventitious root cultures stimulated with 1mM MeJA was 15 days.

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Cite This Article

Deveci, H., Demirci, T., Baydar, N. G. (2022). Effect of culture time on secondary metabolite production from MeJA-stimulated in vitro roots of Hyoscyamus niger L.. International Journal of Secondary Metabolite, Volume: 9 Issue: 2. https://doi.org/10.21448/ijsm.1041324

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