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Journals / International Journal of Secondary Metabolite / 2026 / Volume: 12 Issue: 2

Cytotoxic and apoptotic effect of Lemna minor L. extract on human osteosarcoma (Saos-2)

Sema Yiyit Doğan(GAZI UNIVERSITY)0000-0003-4292-2233
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Publication Date
2025-06-01
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The study suggests L. minor as a promising natural agent for bone cancer treatment.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Osteosarcoma is a malignant form of bone cancer that responds poorly to chemotherapy and has a significant incidence of recurrence.
📊
Key Findings & Data:This study investigated the anticancer activity of the L. minor on Saos-2 cancer cells and the apoptosis pathways.
💡
Conclusion & Impact:The study suggests L. minor as a promising natural agent for bone cancer treatment.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Osteosarcoma · Lemna minor · Apoptosis

Abstract

Osteosarcoma is a malignant form of bone cancer that responds poorly to chemotherapy and has a significant incidence of recurrence. Recent studies suggest the need for new natural agents to support treatment. Lemna minor is a macrophyte frequently used in traditional treatments and is known to have high antioxidant and antimicrobial properties due to the phenolic compounds it contains. This study investigated the anticancer activity of the L. minor on Saos-2 cancer cells and the apoptosis pathways. L. minor was extracted in ethanol by microwave technique. The extract showed cytotoxic activity (59-79%) on Saos-2 cells but did not harm healthy human bone cells (hFOB). Expression of bax, bcl-2, caspase-3, and caspase-8 genes was investigated by RT-PCR to examine the apoptosis-inducing pathways. RT-PCR analysis revealed that L. minor induced apoptosis via a mitochondria-dependent pathway by affecting the bax/bcl-2 ratio. The study suggests L. minor as a promising natural agent for bone cancer treatment.

Özet

Osteosarcoma is a malignant form of bone cancer that responds poorly to chemotherapy and has a significant incidence of recurrence. Recent studies suggest the need for new natural agents to support treatment. Lemna minor is a macrophyte frequently used in traditional treatments and is known to have high antioxidant and antimicrobial properties due to the phenolic compounds it contains. This study investigated the anticancer activity of the L. minor on Saos-2 cancer cells and the apoptosis pathways. L. minor was extracted in ethanol by microwave technique. The extract showed cytotoxic activity (59-79%) on Saos-2 cells but did not harm healthy human bone cells (hFOB). Expression of bax, bcl-2, caspase-3, and caspase-8 genes was investigated by RT-PCR to examine the apoptosis-inducing pathways. RT-PCR analysis revealed that L. minor induced apoptosis via a mitochondria-dependent pathway by affecting the bax/bcl-2 ratio. The study suggests L. minor as a promising natural agent for bone cancer treatment.

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Cite This Article

Doğan, S. Y. (2025). Cytotoxic and apoptotic effect of Lemna minor L. extract on human osteosarcoma (Saos-2). International Journal of Secondary Metabolite, Volume: 12 Issue: 2. https://doi.org/10.21448/ijsm.1489202

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