SavedAdd your journal

Journals / International Journal of Secondary Metabolite / 2026 / Volume: 13 Issue: 1

Determination of essential oil composition of Laurencia obtusa and Ceramium spp. in Black Sea and antileishmanial activity

Tayyibe Beyza Yücel(Giresun Üniversitesi)0000-0002-2632-8325
Seda Fandaklı(ARTVİN ÇORUH ÜNİVERSİTESİ, SAĞLIK HİZMETLERİ MESLEK YÜKSEKOKULU)0000-0002-8199-3336
Ulku Karaman(ORDU ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0001-7027-1613
Şahin Direkel(MALATYA TURGUT ÖZAL ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0003-3368-0447
Pages
—
Publication Date
2026-03-09
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Dichloromethane and chloroform extracts of both species exhibited greater antileishmanial activity compared to methanol and hexane extracts, indicating their higher potential as antileishmanial agents.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, the chemical compositions of volatile oils obtained via Clevenger distillation from red algae species Laurencia obtusa and Ceramium spp., collected from the coastal areas of Sinop (Black Sea), were analyzed by GC/MS.
📊
Key Findings & Data:As a result, 80.92% and 90.51% of the volatile oil compositions of Laurencia obtusa and Ceramium spp. were identified, respectively, with 20 compounds characterized in each species.
💡
Conclusion & Impact:Dichloromethane and chloroform extracts of both species exhibited greater antileishmanial activity compared to methanol and hexane extracts, indicating their higher potential as antileishmanial agents.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Laurencia obtusa · Ceramium spp. · GC-MS

Abstract

In this study, the chemical compositions of volatile oils obtained via Clevenger distillation from red algae species Laurencia obtusa and Ceramium spp., collected from the coastal areas of Sinop (Black Sea), were analyzed by GC/MS. Additionally, hexane, dichloromethane, chloroform, and methanol extracts were prepared using the Soxhlet extraction method, and their antileishmanial activities were evaluated. As a result, 80.92% and 90.51% of the volatile oil compositions of Laurencia obtusa and Ceramium spp. were identified, respectively, with 20 compounds characterized in each species. Hydrocarbons were the predominant compound class in the essential oils of Laurencia obtusa (79.31%) and Ceramium spp. (72.89%). The leishmanicidal activities of the solvent extracts were tested in vitro against Leishmania infantum promastigotes. The minimum inhibitory concentration (MIC) values for Laurencia obtusa extracts in hexane, dichloromethane, chloroform, and methanol were 5, 2.5, 2.5, and 10 mg/mL, respectively. For Ceramium spp., the MIC values were 2.5, 2.5, 2.5, and 10 mg/mL, respectively. Dichloromethane and chloroform extracts of both species exhibited greater antileishmanial activity compared to methanol and hexane extracts, indicating their higher potential as antileishmanial agents.

Özet

In this study, the chemical compositions of volatile oils obtained via Clevenger distillation from red algae species Laurencia obtusa and Ceramium spp., collected from the coastal areas of Sinop (Black Sea), were analyzed by GC/MS. Additionally, hexane, dichloromethane, chloroform, and methanol extracts were prepared using the Soxhlet extraction method, and their antileishmanial activities were evaluated. As a result, 80.92% and 90.51% of the volatile oil compositions of Laurencia obtusa and Ceramium spp. were identified, respectively, with 20 compounds characterized in each species. Hydrocarbons were the predominant compound class in the essential oils of Laurencia obtusa (79.31%) and Ceramium spp. (72.89%). The leishmanicidal activities of the solvent extracts were tested in vitro against Leishmania infantum promastigotes. The minimum inhibitory concentration (MIC) values for Laurencia obtusa extracts in hexane, dichloromethane, chloroform, and methanol were 5, 2.5, 2.5, and 10 mg/mL, respectively. For Ceramium spp., the MIC values were 2.5, 2.5, 2.5, and 10 mg/mL, respectively. Dichloromethane and chloroform extracts of both species exhibited greater antileishmanial activity compared to methanol and hexane extracts, indicating their higher potential as antileishmanial agents.

🔖

Cite This Article

Yücel, T. B., Fandaklı, S., Karaman, U., Direkel, Ş. (2026). Determination of essential oil composition of Laurencia obtusa and Ceramium spp. in Black Sea and antileishmanial activity. International Journal of Secondary Metabolite, Volume: 13 Issue: 1. https://doi.org/10.21448/ijsm.1590824

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (23)

  1. Amang à Ngnoung, G.A., Nganso, D.Y.O., Leutcha, P.B., Dize, D., Tatsimo, S.J.N., Tchokouaha, L.R.Y., Kowa, T.K., Tembeni, B., Mamoudou, H., Poka, M., Demana, P.H., Noundou, S.X., Boyom, F.F., & Lannang, M.A. (2023). Antiplasmodial and antileishmanial activities of a new limonoid and other constituents from the stem bark of Khaya senegalensis. Molecules, 28(20), 7227. https://doi.org/10.3390/molecules28207227
  2. Aydoğmuş, Z., Imre, S., Ersoy, L., & Wray, V. (2004). Halogenated secondary metabolites from Laurencia obtusa. Natural Product Research, 18(1), 43 49. https://doi.org/10.1080/1057563031000122086
  3. Cortés, Y., Hormazábal, E., Leal, H., Urzúa, A., Mutis, A., Parra, L., & Quiroz, A. (2014). Novel antimicrobial activity of a dichloromethane extract obtained from red seaweed Ceramium rubrum (Hudson) (Rhodophyta: Florideophyceae) against Yersinia ruckeri and Saprolegnia parasitica, agents that cause diseases in salmonids. Electronic Journal of Biotechnology, 17(3), 126–131. https://doi.org/10.1016/j.ejbt.2014.04.005
  4. Dellatorre, F.G., Avaro, M.G., Commendatore, M.G., Arce, L., & Vivar, M.E.D. (2020). The macroalgal ensemble of Golfo Nuevo (Patagonia, Argentina) as a potential source of valuable fatty acids for nutritional and nutraceutical purposes. Algal Research, 45, 101726. https://doi.org/10.1016/j.algal.2019.101726
  5. Demirel, Z., Koz, Y., Yavaşoğlu, N.U., Özdemir, G., & Sukatar, A. (2011). Antimicrobial and antioxidant activities of solvent extracts and the essential oil composition of Laurencia obtusa and Laurencia obtusa var. pyramidata. Romanian Biotechnological Letters, 16(11), 5927–5936.
  6. Dodds, W.K., & Whiles, M.R. (2019). Freshwater ecology: Concepts and environmental applications of limnology (3rd ed.). Academic Press.
  7. Dubber, D., & Harder, T. (2008). Extracts of Ceramium rubrum, Mastocarpus stellatus and Laminaria digitata inhibit growth of marine and fish pathogenic bacteria at ecologically realistic concentrations. Aquaculture, 274(2–4), 196–200. https://doi.org/10.1016/j.aquaculture.2007.11.029
  8. Esselin, H., Sutour, S., Liberal, J., Cruz, M.T., Salgueiro, L., Siegler, B., Freuze, I., Castola, V., Paoli, M., Bighelli, A., & Tomi, F. (2017). Chemical composition of Laurencia obtusa extract and isolation of a new C15-acetogenin. Molecules, 22(5), 779. https://doi.org/10.3390/molecules22050779
  9. Herwaldt, B.L. (1999). Miltefosine—the long-awaited therapy for visceral leishmaniasis. New England Journal of Medicine, 341(24), 1840–1842. https://doi.org/10.1056/NEJM199912093412411
  10. Karabay-Yavaşoğlu, N.U., Sukatar, A., Ozdemir, G., & Horzum, Z. (2007). Antimicrobial activity of volatile components and various extracts of the red alga Jania rubens. Phytotherapy Research, 21, 153–156. https://doi.org/10.1002/ptr.2045
  11. Karaman, Ü., Yücel, T.B., Cebeci, T., Arici, Y.K., Yücel, M., & Korkmaz, B. (2022). Investigation of biological, scolicidal and antimicrobial activities of the solvent extracts components of Cystoseira barbata seen off Sinop Province. Indian Journal of Pharmaceutical Education and Research, 56(2), 312–325. https://doi.org/10.5530/ijper.56.2s.102
  12. Khattab, S.N., Haiba, N.S., Asal, A.M., Bekhit, A.A., Guemei, A.A., Amer, A., & ElFaham, A. (2017). Study of antileishmanial activity of 2-aminobenzoyl amino acid hydrazides and their quinazoline derivatives. Bioorganic & Medicinal Chemistry Letters, 27(4), 918 921. https://doi.org/10.1016/j.bmcl.2017.01.003
  13. König, G.M., & Wright, A.D. (1997). Sesquiterpene content of the antibacterial dichloromethane extract of the marine red alga Laurencia obtusa. Planta Medica, 63(2), 186 187. https://doi.org/10.15625/1859-3097/18/2/8998
  14. Malmvärn, A., Marsh, G., Kautsky, L., Athanasiadou, M., Bergman, Å., & Asplund, L. (2005). Hydroxylated and methoxylated brominated diphenyl ethers in the red algae Ceramium tenuicorne and blue mussels from the Baltic Sea. Environmental Science & Technology, 39(9), 2990–2997. https://doi.org/10.1021/es0482886
  15. Ozgun, G., Kayalar, H., Alaylı, F., Cavus, İ., Donmez, F., & Ozbilgin, A. (2024). Antileishmanial efficacy of Heracleum persicum Desf. ex. Fisch. extracts against Leishmania tropica promastigotes. Universal Journal of Pharmaceutical Research, 9(4), 1–4. https://doi.org/10.22270/ujpr.v9i4.1154
  16. Sanjeewa, K.K.A., Kim, E.A., Son, K.T., & Jeon, Y.J. (2016). Bioactive properties and potentials cosmeceutical applications of phlorotannins isolated from brown seaweeds: A review. Journal of Photochemistry and Photobiology B: Biology, 162, 100 105. https://doi.org/10.1016/j.jphotobiol.2016.06.027
  17. Süleymanoğlu, N., Ustabaş, R., Direkel, Ş., Alpaslan, Y.B., & Ünver, Y. (2018). 1,2,4-Triazole derivatives with morpholine; DFT study and antileishmanial activity. Canadian Journal of Physics, 96(7), 719–723. https://doi.org/10.1139/cjp-2017-0710
  18. Wu, J.Y., Tso, R., Teo, H.S., & Haldar, S. (2023). The utility of algae as sources of high value nutritional ingredients, particularly for alternative/complementary proteins to improve human health. Frontiers in Nutrition, 10, 1277343. https://doi.org/10.3389/fnut.2023.1277343
  19. Yesilova, K., Balkis, N., & Taskin, E. (2017). Seasonal investigation of the protein, carbohydrate and lipid contents of dominant macroalgae on the western coasts of the Black Sea. Fresenius Environmental Bulletin, 26, 46–55.
  20. Yıldırım, A., Aksoy, T., Kayalar, H., & Balcıoğlu, İ.C. (2024). Semen cannabis and Oleum hyperici: Antileishmanial activity against Leishmania tropica promastigotes and intracellular amastigote. Parasitology International, 103, 102950. https://doi.org/10.1016/j.parint.2024.102950
  21. Yücel, T.B. (2022). Comparison of volatile chemical components of Cystoseira crinita Duby by hydrodistillation (HD) and solid-phase microextraction (SPME) methods and antimicrobial and scolicidal activities of essential oil and extracts. Turkish Journal of Chemistry, 46, 378–393. https://doi.org/10.55730/1300-0527.3314
  22. Yücel, T.B., Korkmaz, B., Cebeci, T., Karaman, Ü., Arici, Y.K., Fandaklı, S., & Gul, T. (2023). Analysis of volatile constituent by hydrodistillation and solid-phase microextraction techniques and antimicrobial and scolicidal activities of essential oil and Soxhlet extracts of Ulva rigida grown in Turkey. Chemistry & Biodiversity, 20, e202300965. https://doi.org/10.1002/cbdv.202300965
  23. Zerrifi, S.A., El Khalloufi, F., Oudra, B., & Vasconcelos, V. (2018). Seaweed bioactive compounds against pathogens and microalgae: Potential uses on pharmacology and harmful algae bloom control. Marine Drugs, 16(2), 55. https://doi.org/10.3390/md16020055