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Journals / International Journal of Secondary Metabolite / 2026 / Volume: 13 Issue: 2

Effects of extraction techniques on bioactive compounds and biological properties of Silene behen L.

Sengul Uysal(ERCİYES ÜNİVERSİTESİ)0000-0003-4562-1719
Kouadio Ibrahime Sinan(SELÇUK ÜNİVERSİTESİ)0000-0002-6168-5707
Gökhan Zengin(SELCUK UNIVERSITY)0000-0001-6548-7823
Pages
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Publication Date
2026-03-10
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The antioxidant results indicated that UAE can be used to extract natural antioxidant agents from S. behen.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The present research evaluated the maceration and ultrasonication-assisted extraction methods on the phytochemical composition, antioxidant activity, and enzyme inhibition effects of Silene behen.
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Key Findings & Data:The spectrophotometric methods determined the total bioactive compounds. 22 phenolic compounds were analyzed in S. behen extracts.
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Conclusion & Impact:The antioxidant results indicated that UAE can be used to extract natural antioxidant agents from S. behen.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:S. behen · Extraction methods · Phenolic

Abstract

The present research evaluated the maceration and ultrasonication-assisted extraction methods on the phytochemical composition, antioxidant activity, and enzyme inhibition effects of Silene behen. The spectrophotometric methods determined the total bioactive compounds. 22 phenolic compounds were analyzed in S. behen extracts. Benzoic acid was the major phenolic compound (S. behen MAC: 3552.7 µg/g; S. behen UAE: 4215.2 µg/g), followed by gallic acid and epicatechin. S. behen UAE (19.47 mg GAE/g) contained higher total phenolic content compared to S. behen MAC (16.57 mg GAE/g) extract. Similarly, S. behen UAE showed higher antioxidant activity than S. behen MAC. S. behen UAE showed the strongest radical scavenging activity, with DPPH (31.66 mg TE/g) and ABTS (49.54 mg TE/g). The highest reducing power activity was found in the S. behen UAE (CUPRAC: 96.56 mgTE/g; FRAP: 36.29 mgTE/g). The antioxidant results indicated that UAE can be used to extract natural antioxidant agents from S. behen. The tested extracts displayed inhibitory effects against tyrosinase, cholinesterase, α-amylase, and α-glucosidase. S. behen UAE (2.38 mg GALAE/g) demonstrated the highest activity against butyrylcholinesterase enzyme. Inhibition of α-amylase and α-glucosidase was similar across the two extracts. The anti-tyrosinase effect of S. behen MAC (47.49 mgKAE/g) was higher than that of S. behen UAE (45.65 mgKAE/g). These results highlight that S. behen extract may serve as a promising source of natural antioxidants and enzyme inhibitors.

Özet

The present research evaluated the maceration and ultrasonication-assisted extraction methods on the phytochemical composition, antioxidant activity, and enzyme inhibition effects of Silene behen. The spectrophotometric methods determined the total bioactive compounds. 22 phenolic compounds were analyzed in S. behen extracts. Benzoic acid was the major phenolic compound (S. behen MAC: 3552.7 µg/g; S. behen UAE: 4215.2 µg/g), followed by gallic acid and epicatechin. S. behen UAE (19.47 mg GAE/g) contained higher total phenolic content compared to S. behen MAC (16.57 mg GAE/g) extract. Similarly, S. behen UAE showed higher antioxidant activity than S. behen MAC. S. behen UAE showed the strongest radical scavenging activity, with DPPH (31.66 mg TE/g) and ABTS (49.54 mg TE/g). The highest reducing power activity was found in the S. behen UAE (CUPRAC: 96.56 mgTE/g; FRAP: 36.29 mgTE/g). The antioxidant results indicated that UAE can be used to extract natural antioxidant agents from S. behen. The tested extracts displayed inhibitory effects against tyrosinase, cholinesterase, α-amylase, and α-glucosidase. S. behen UAE (2.38 mg GALAE/g) demonstrated the highest activity against butyrylcholinesterase enzyme. Inhibition of α-amylase and α-glucosidase was similar across the two extracts. The anti-tyrosinase effect of S. behen MAC (47.49 mgKAE/g) was higher than that of S. behen UAE (45.65 mgKAE/g). These results highlight that S. behen extract may serve as a promising source of natural antioxidants and enzyme inhibitors.

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Cite This Article

Uysal, S., Sinan, K. I., Zengin, G. (2026). Effects of extraction techniques on bioactive compounds and biological properties of Silene behen L.. International Journal of Secondary Metabolite, Volume: 13 Issue: 2. https://doi.org/10.21448/ijsm.1775802

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