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AI InsightThe isol'ated new strain was positive in, 1/ 10.000 titre against Leptospira grippotyphosa immun serum when tested with M.A test.
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Abstract
Blood samples were obtained from one of the 65 cattle having fever and icterrohaemoglobinuriae atafarmin Haymana, Ankara. Its Blood and urine were inoculated into six-week old hamsters by the subcutaneous route a:O.d into Fletchers, aıl"d Chang Media. After 29 days of ineubatian a strain was isolated in Fletcher's medium. Bovin urine inoculeted into hamster, leptospira were found in t~ir kidney and isolated a new strain The strain were found pozitive in M.A. test against leptospira grippotyphosa irnmun serum in 1/~400 titre when sera from the 65 cattle tested examination by M.A test gave pozitive result against Leptospira grippotyphosa in 1/ 200-1/3200 titre. From these cases, three cows belonging to this form died. Case II Blood serum and kidney samples from two cattle sent to our laboratory f:rom Keskin, Ankara were.examined for leptospirosis. Serum samples were examined by Micro Agglutination test CM.AJ the sera were found positive against Leptospira grippotyphosa in 1/1600 titre. Tissue emulsions of kidneys were inoculated into Fletcher's, and Chang media and to 6 weeks old 6 hamsters, subcutaneously and intra peritoneally. The leptospira organism were isolated in Fletcher's and Chang media and were identified under the dark field microscope. The two hamsters which were inoculated intra peritoneally died within 13 days after inoculation kidney emufsion ·from the dead hamsters were inoculacted into anather set off hamsters for the pathogenicty control. The isol'ated new strain was positive in, 1/ 10.000 titre against Leptospira grippotyphosa immun serum when tested with M.A test.
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