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Journals / Çukurova Anestezi ve Cerrahi Bilimler Dergisi / 2026 / Cilt: 7 Sayı: 1

Effect of Biochanin-A on The Cerebellum in Cerebral Ischemia Reperfusion Injury

SEREBRAL İSKEMİ REPERFÜZYON HASARINDA BİYOKANİN-A'NIN SEREBELLUM ÜZERİNDEKİ ETKİSİ

Öner Avınca(DİYARBAKIR GAZİ YAŞARGİL SAĞLIK UYGULAMA VE ARAŞTIRMA MERKEZİ)0000-0003-3109-6773
Özge Kaplan(KOCAELI UNIVERSITY, INSTITUTE OF HEALTH SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY)0000-0001-5203-9650
Fırat Aşır(DİCLE ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, HİSTOLOJİ VE EMBRİYOLOJİ ANABİLİM DALI)0000-0002-6384-9146
Pages
47–51
Publication Date
2024-03-11
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: We suggest that Biochanin A treatment has a potential therapeutic role in alleviating inflammation in the cerebellum after cerebral ischemia reperfusion.

📌 3 Key Takeaways

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Objective & Hypothesis:ABSTRACT Objective: To examine the protective effect of Biochanin A (BCA) on the cerebellum in cerebral ischemia reperfusion injury.
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Key Findings & Data:Material and Metodhs: 24 Wistar albino female rats were divided into 3 groups.
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Conclusion & Impact:Conclusion: We suggest that Biochanin A treatment has a potential therapeutic role in alleviating inflammation in the cerebellum after cerebral ischemia reperfusion.
🔬Design:Klinik Araştırma
📍Scope:Comprehensive Sample
⚙️Variables:Serebral iskemi · Biyokanin A · S100

Abstract

ABSTRACT Objective: To examine the protective effect of Biochanin A (BCA) on the cerebellum in cerebral ischemia reperfusion injury. Material and Metodhs: 24 Wistar albino female rats were divided into 3 groups. Control, Ischemia reperfusion (IR), IR +BCA (20 mg/kg intraperitoneal) group. A micro bulldog clamp was placed on the left common carotid artery of the rats and cerebral ischemia was applied for 2 hours. After cerebral ischemia, the clamp was removed and reperfusion was performed for 24 hours. After 7 days, all rats were decapitated and the protective effects of Biochanin A on the cerebellum were evaluated by immunohistochemically analyses. Results: In the IR group; S100 expression was also observed positive in neurons and neuroglia in the pia mater, molecular layer, Purkinje cells in the ganglionic layer and granule cells in the granular layer, and neuroglia in the white matter. In the IR+ Biochanin A group, it was observed that the immune activity in the granule cells and Purkinje cells in the granular layer was reduced compared to the IR group. Conclusion: We suggest that Biochanin A treatment has a potential therapeutic role in alleviating inflammation in the cerebellum after cerebral ischemia reperfusion.

Özet

ÖZET Amaç:Serebral iskemi reperfüzyon hasarında Biochanin A'nın (BCA) serebellum üzerindeki koruyucu etkisini incelemek. Materyal ve Metod: 24 Wistar albino dişi sıçan 3 gruba ayrıldı. Kontrol, İskemi reperfüzyon (İR), İR +BCA (20 mg/kg intraperitoneal) grubu. Sıçanların sol ana karotid arterine bir mikro bulldog klemp yerleştirildi ve 2 saat boyunca serebral iskemi uygulandı. Serebral iskemi sonrasında klemp çıkarıldı ve 24 saat boyunca reperfüzyon uygulandı. 7 gün sonra tüm sıçanlar dekapite edildi ve Biochanin A'nın serebellum üzerindeki koruyucu etkileri immünohistokimyasal analizlerle değerlendirildi. Bulgular: IR grubunda; pia mater, moleküler tabaka, ganglionik tabakada Purkinje hücreleri ve granüler tabakada granül hücreleri ve beyaz cevherde nörogliadaki nöron ve nöroglialarda S100 ekspresyonu pozitif olarak gözlendi. IR+ Biyokanin A grubunda, granül hücrelerdeki ve granüler tabakadaki Purkinje hücrelerindeki immün aktivitenin IR grubuna kıyasla azaldığı gözlenmiştir. Sonuç: Biochanin A tedavisinin serebral iskemi reperfüzyonundan sonra serebellumdaki enflamasyonu hafifletmede potansiyel bir terapötik rolü olduğunu düşünüyoruz.

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Cite This Article

Avınca, Ö., Kaplan, Ö., Aşır, F. (2024). Effect of Biochanin-A on The Cerebellum in Cerebral Ischemia Reperfusion Injury. Çukurova Anestezi ve Cerrahi Bilimler Dergisi, Cilt: 7 Sayı: 1, 47–51. https://doi.org/10.36516/jocass.1425526

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