Reprodüktif ve Menopozal Servikal Smear ve Kan Örneklerinde DNA Hasarı Tespitinin Değerlendirilmesi

Conclusion: The reasons for the high level of DNA damage and MN scoring during menopause may be hormonal changes seen with aging, increased oxidative stress, weakening of DNA repair mechanisms and epigenetic changes.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: As we age, DNA damage occurs in the body due to slowing of the metabolic rate, changes in hormone levels and increased oxidative stress.

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Key Findings & Data:Material and Methods: Lymphocyte samples from 30 women of reproductive age and 30 menopausal women were evaluated for DNA damage using the comet assay, smear samples were evaluated for micronucleus (MN) cells, a morphological marker of chromosomal instability using the MN assay, and binucleated (BN) and karyorrhectic (KR) cells were evaluated for degenerative nuclear changes.

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Conclusion & Impact:Conclusion: The reasons for the high level of DNA damage and MN scoring during menopause may be hormonal changes seen with aging, increased oxidative stress, weakening of DNA repair mechanisms and epigenetic changes.

🔬Design:Araştırma Makalesi

📍Scope:Comprehensive Sample

⚙️Variables:Konvansiyonel servikal smear · comet testi · DNA hasarı

Abstract

Aim: As we age, DNA damage occurs in the body due to slowing of the metabolic rate, changes in hormone levels and increased oxidative stress. This study aimed to investigate DNA damage and degenerative nuclear changes in women at different times. Material and Methods: Lymphocyte samples from 30 women of reproductive age and 30 menopausal women were evaluated for DNA damage using the comet assay, smear samples were evaluated for micronucleus (MN) cells, a morphological marker of chromosomal instability using the MN assay, and binucleated (BN) and karyorrhectic (KR) cells were evaluated for degenerative nuclear changes. Results: DNA damage in blood samples was found to be higher in the menopausal period compared to the reproductive period by comet assay (p 0.05). Conclusion: The reasons for the high level of DNA damage and MN scoring during menopause may be hormonal changes seen with aging, increased oxidative stress, weakening of DNA repair mechanisms and epigenetic changes.

Özet

Amaç: Yaşlandıkça, metabolizma hızının yavaşlaması, hormon seviyelerindeki değişiklikler ve artan oksidatif stres nedeniyle vücutta DNA hasarı meydana gelir. Bu çalışma, kadınların farklı dönemlerinde DNA hasarını ve dejeneratif nükleer değişiklikleri araştırmayı amaçlamıştır. Gereç ve Yöntemler: Reprodüktif döneminde 30 kadın ve menopoz döneminde 30 kadından alınan lenfosit örnekleri komet testi kullanılarak DNA hasarı açısından değerlendirilmiş, smear örnekleri MN testi kullanılarak kromozomal instabilitenin morfolojik bir belirteci olan mikronükleus (MN) hücreleri açısından, binükleus (BN) ve karyorektik (KR) hücreler ise dejeneratif nükleer değişiklikler açısından değerlendirilmiştir. Bulgular: Kan örneklerindeki DNA hasarı comet assay ile menopozal dönemde üreme dönemine kıyasla daha yüksek bulundu (p 0.05). Sonuç: Menopoz döneminde DNA hasarı ve MN skorlamasının yüksek bulunması nedenleri arasında yaşlanma ile birlikte görülen hormonal değişimler, oksidatif stres artışı, DNA onarım mekanizmalarının zayıflaması ve epigenetik değişiklikler olabilir.

Keywords

Konvansiyonel servikal smear comet testi DNA hasarı menopoz mikronükleus testi Conventional cervical smear comet assay DNA damage menopause micronucleus test

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Cite This Article

Sancer, O., Koşar, P. A., Öz, Z. S., Tola, E. N., Toğay, A., Çiriş, İ. M., Tepebaşı, M. Y. (2025). Reprodüktif ve Menopozal Servikal Smear ve Kan Örneklerinde DNA Hasarı Tespitinin Değerlendirilmesi. Batı Karadeniz Tıp Dergisi, Cilt: 9 Sayı: 2, 175–180. https://doi.org/10.29058/mjwbs.1589487

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