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Journals / Balkan Medical Journal / 2026 / Cilt: 33 Sayı: 2

Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation

Kevser Erol(Department of Pharmacology, Osmangazi University School of Medicine, Eskişehir, Turkey)
Semra Yiğitaslan(Department of Pharmacology, Osmangazi University School of Medicine, Eskişehir, Turkey)
Çiğdem Ünel(Department of Pharmacology, Osmangazi University School of Medicine, Eskişehir, Turkey)
Bilgin Kaygısız(Department of Pharmacology, Osmangazi University School of Medicine, Eskişehir, Turkey)
Engin Yıldırım(Department of Pharmacology, Osmangazi University School of Medicine, Eskişehir, Turkey)
Pages
0–0
Publication Date
2016-03-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Voltage-dependent calcium channels may play a role in cisplatin neurotoxicity.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Background: Calcium homeostasis is considered to be important in antineoplastic as well as in neurotoxic adverse effects of cisplatin.
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Key Findings & Data:Aims: This study aimed to investigate the role of Ca2+ in cisplatin neurotoxicity in cultured rat dorsal root ganglia (DRG) cells.
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Conclusion & Impact:Conclusion: Voltage-dependent calcium channels may play a role in cisplatin neurotoxicity.
🔬Design:Makale
📍Scope:Comprehensive Sample
⚙️Variables:Calcium · cisplatin · dorsal root ganglia

Abstract

Background: Calcium homeostasis is considered to be important in antineoplastic as well as in neurotoxic adverse effects of cisplatin. Aims: This study aimed to investigate the role of Ca2+ in cisplatin neurotoxicity in cultured rat dorsal root ganglia (DRG) cells. Study Design: Cell culture study. Methods: DRG cells prepared from 1-day old Sprague-Dawley rats were used to determine the role of Ca2+ in the cisplatin (10-600 μM) neurotoxicity. The cells were incubated with cisplatin plus nimodipine (1-3 μM), dizocilpine (MK-801) (1-3 μM) or thapsigargin (100-300 nM). Toxicity of cisplatinon DRG cells was determined by the MTT assay. Results: The neurotoxicity of cisplatin was significant when used in high concentrations (100-600 μM). Nimodipine (1 μM) but not MK-801 or thapsigargin prevented the neurotoxic effects of 200 μM of cisplatin. Conclusion: Voltage-dependent calcium channels may play a role in cisplatin neurotoxicity.

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Cite This Article

Erol, K., Yiğitaslan, S., Ünel, Ç., Kaygısız, B., Yıldırım, E. (2016). Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation. Balkan Medical Journal, Cilt: 33 Sayı: 2, 0–0. https://ajindex.com/articles/1373773

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