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Journals / Bahçe / 2026 / Cilt: 54 Sayı: 1

Determination of Plant Regeneration Protocol of the ‘Viking’ Aronia Cultivar under In Vitro Conditions

‘Viking’ Aronya Çeşidinin In Vitro Şartlarda Bitki Rejenerasyon Protokolünün Belirlenmesi

Zeynep Nas(Selçuk Üniversitesi)0000-0002-7115-1987
Ahmet Eşitken(SELÇUK ÜNİVERSİTESİ)0000-0002-6140-7782
Lütfi Pırlak(SELÇUK ÜNİVERSİTESİ)0000-0003-3630-3591
Journal
Bahçe
Pages
11–16
Publication Date
2025-05-27
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The acclimatization phase has resulted in high success, 98% of plants in ½MS medium and 92.4% of plants in MS medium survived.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, in vitro propagation of ‘Viking’ aronia cultivar was investigated.
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Key Findings & Data:For this purpose, different cytokinin types (meta-Topolin, Benzyladenine, Thidiazuron, Kinetin) and concentrations (0.5, 1.0 mg.l⁻¹) were tested in MS nutrient medium for propagation medium.
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Conclusion & Impact:The acclimatization phase has resulted in high success, 98% of plants in ½MS medium and 92.4% of plants in MS medium survived.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:aronya · in vitro · köklenme

Abstract

In this study, in vitro propagation of ‘Viking’ aronia cultivar was investigated. For this purpose, different cytokinin types (meta-Topolin, Benzyladenine, Thidiazuron, Kinetin) and concentrations (0.5, 1.0 mg.l⁻¹) were tested in MS nutrient medium for propagation medium. Except for cytokinins, GA₃ (0.25 mg.l⁻¹) and low concentration of IBA (0.01 mg.l⁻¹) were added and equal concentrations were used in all treatments. For the rooting medium, different concentrations of IBA (0.125, 0.25, 0.5, 1.0 mg.l⁻¹) and activated charcoal (0.5, 1.0 g.l⁻¹) were tested in MS and ½MS media. During the study, plant vitality, shoot number, shoot length, rooting rate, root number and root length were measured. At the end of the study, 100% viability was detected in all treatments. The highest number of shoots was observed in media containing TDZ; however, most of the shoots that can be used as micro cuttings were obtained from media containing BA and mT. The highest number of shoots occurred at 1.0 mg.l⁻¹ concentrations of media containing BA and mT and 16.83 and 21.76 shoots/explants were obtained, respectively. 100% rooting was achieved in all treatments, including the control. The highest root number was observed in ½MS medium (10.17 roots/explant) containing 1.0 mg.l⁻¹ IBA. Higher root number was obtained in ½MS medium compared to MS medium; However, it was determined that the root length decreased with the increase in the root number. The acclimatization phase has resulted in high success, 98% of plants in ½MS medium and 92.4% of plants in MS medium survived.

Özet

Bu çalışmada ‘Viking’ aronya çeşidinin in vitro çoğaltımı araştırılmıştır. Bu amaçla çoğaltma ortamı olarak MS besin ortamında farklı sitokinin tipleri (meta-Topolin, Benzyladenine, Thidiazuron, Kinetin) ve dozları (0.5, 1.0 mg.l⁻¹) denenmiştir. Sitokininler dışında ortama GA₃ (0.25 mg.l⁻¹) ve düşük dozda IBA (0.01 mg.l⁻¹) ilave edilmiş ve tüm uygulamalarda eşit doz kullanılmıştır. Köklenme ortamı için MS ve ½MS ortamlarında, farklı dozlarda IBA (kontrol, 0.125, 0.25, 0.5, 1.0 mg.l⁻¹) ve aktif kömür (0.5, 1.0 g.l⁻¹) denenmiştir. Çalışma sonunda bitki canlılığı, explant başına sürgün sayısı (adet), sürgün uzunluğu (cm), köklenme oranı (%), kök sayısı (adet) ve kök uzunluğu (cm) ölçülmüştür. Çalışma sonunda, tüm uygulamalarda %100 canlılık tespit edilmiştir. En yüksek sürgün sayısı TDZ içeren ortamlarda tespit edilmiş; fakat mikro çelik olarak kullanılabilecek sürgünlerin çoğu BA ve mT içerikli ortamlardan elde edilmiştir. BA ve mT içerikli ortamların 1 mg.l⁻¹ dozlarında en yüksek sürgün sayısı meydana gelmiş ve sırasıyla 16.83 ve 21.76 adet sürgün/eksplant elde edilmiştir. Kontrol dahil olmak üzere tüm uygulamalarda %100 köklenme gerçekleşmiştir. En yüksek kök sayısı 1.0 mg.l⁻¹ IBA içeren ½MS ortamında (10.17 kök/eksplant) tespit edilmiştir. ½MS ortamında, MS ortamına kıyasla daha yüksek kök sayısı elde edilmiş; fakat kök sayısının artmasıyla kök uzunluğunda kısalma tespit edilmiştir. Aklimatizasyon aşaması yüksek başarı ile sonuçlanmış, ½MS ortamındaki bitkilerin %98’i, MS ortamındakilerin ise %92.4’ü hayatta kalmıştır.

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Cite This Article

Nas, Z., Eşitken, A., Pırlak, L. (2025). Determination of Plant Regeneration Protocol of the ‘Viking’ Aronia Cultivar under In Vitro Conditions. Bahçe, Cilt: 54 Sayı: 1, 11–16. https://doi.org/10.53471/bahce.1618731

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