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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2025 / Volume: 72 Issue: 4

Phylogeographic distribution of Bacillus anthracis strains from humans and animals in Türkiye: Genotyping based on MLVA-31 loci analysis (2010–2021)

Süleyman Yalçın(Sağlık bakanlığı Halk Sağlığı Genel Müdürlüğü Mikrobiyoloji Referans Laboratuvarı)0000-0003-1434-2717
Bülent Acar(Halk Sağlığı Genel Müdürlüğü)0009-0008-2042-5512
Selahattin Şen(Etlik Veteriner Kontrol ve Araştırma Enstitüsü)0000-0003-0841-0103
Selçuk Kılıç(SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, SAVUNMA SAĞLIK BİLİMLERİ ENSTİTÜSÜ, TIBBİ KİMYASAL BİYOLOJİK RADYOLOJİK VE NÜKLEER SAVUNMA ANABİLİM DALI)0000-0002-4993-650X
Barış Sareyyüpoğlu(ANKARA ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, KLİNİK ÖNCESİ BİLİMLERİ BÖLÜMÜ, VETERİNERLİK MİKROBİYOLOJİSİ ANABİLİM DALI)0000-0002-2212-2610
Pages
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Publication Date
2025-10-01
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Identifying and monitoring the local, regional, and international genetic characterization of B. anthracis is essential in combating potential anthrax outbreaks, which can be a public health issue.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This molecular microbiological study aimed to investigate the genotype characterization of Bacillus anthracis (B. anthracis) strains circulating in Türkiye.
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Key Findings & Data:A total of 114 B. anthracis strains [82 from animal isolates, 28 from human isolates, 3 Refik Saydam Culture Collection (RSKK) strains, and 1 Max Sterne vaccine strain] from 2010 to 2021 were included in the study.
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Conclusion & Impact:Identifying and monitoring the local, regional, and international genetic characterization of B. anthracis is essential in combating potential anthrax outbreaks, which can be a public health issue.
🔬Design:Research Article
📍Scope:2010 Verileri
⚙️Variables:Bacillus anthracis · Genotyping · MLVA-31

Abstract

This molecular microbiological study aimed to investigate the genotype characterization of Bacillus anthracis (B. anthracis) strains circulating in Türkiye. A total of 114 B. anthracis strains [82 from animal isolates, 28 from human isolates, 3 Refik Saydam Culture Collection (RSKK) strains, and 1 Max Sterne vaccine strain] from 2010 to 2021 were included in the study. The Multiple-locus Variable Number Tandem Repeat Analysis (MLVA) was performed using MLVA 31-loci. A total of 70 genotypes were revealed, including 4 different clusters (A, C1, C2, and C3), and there was 1 genotype in cluster A, 8 genotypes in cluster C1, 40 genotypes in cluster C2, and 21 genotypes in cluster C3. The C2 cluster was determined to be the most dominant cluster (68.4%) among all strains. The G18 (Genotype 18 contained 20 strains from 13 provinces in 4 geographical regions) was revealed to be the most common among the genotypes in the C2 cluster. It was observed that the same genotype can be found from different origins, in different cities, and in different years. The investigation of the temporal and spatial distribution of B. anthracis genotypes throughout a country could determine the possible origin of the cases. Furthermore, it has been shown that the discriminatory power of the MLVA-31 method is higher than that of the previous MLVA-25 method. Identifying and monitoring the local, regional, and international genetic characterization of B. anthracis is essential in combating potential anthrax outbreaks, which can be a public health issue.

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Cite This Article

Yalçın, S., Acar, B., Şen, S., Kılıç, S., Sareyyüpoğlu, B. (2025). Phylogeographic distribution of Bacillus anthracis strains from humans and animals in Türkiye: Genotyping based on MLVA-31 loci analysis (2010–2021). Ankara Üniversitesi Veteriner Fakültesi Dergisi, Volume: 72 Issue: 4. https://doi.org/10.33988/auvfd.1671839

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