Journals / Akademik Gıda / 2026 / Cilt: 23 Sayı: 1

Extraction of Phenolic Compounds from Pomegranate Peels by Ultrasound Assisted Extraction and Determination of Antioxidant Activities

Nar Kabuğundan Ultrason Destekli Ekstraksiyon Uygulamasıyla Fenolik Bileşen Ekstraksiyonu ve Ekstraktların Antioksidan Aktiviteleri

Buse Özdere(Ege Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü)0009-0007-9929-9522
Özge Ata(Ege Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü)0000-0001-9329-5474
Şeyda Mihriban Kul(Ege Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü)0009-0007-2146-126X
Bilge Baştürk Berk(Ege Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü)0000-0002-7405-5288
Şebnem Tavman(Ege Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü)0000-0002-3069-1709
Seher Kumcuoğlu(Ege Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü)0000-0002-3663-2881
Pages
47–59
Publication Date
2025-05-11
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed that the outer peel exhibited the highest total phenolic content, reaching 58.73 ± 0.79 mg GAE/g DM under 100% power and 10 minutes of extraction.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of this study was to extract bioactive compounds from pomegranate peels (Punica granatum L.) using the ultrasound-assisted extraction (UDE) method within a zero-waste approach and to investigate the effects of different extraction conditions on total phenolic content and antioxidant capacity.
📊
Key Findings & Data:Fresh pomegranate peels were separated into inner and outer layers, and extractions were performed using 80% methanol as a solvent under different ultrasound power levels (50% and 100%) and durations (5, 10, and 20 minutes).
💡
Conclusion & Impact:The results showed that the outer peel exhibited the highest total phenolic content, reaching 58.73 ± 0.79 mg GAE/g DM under 100% power and 10 minutes of extraction.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Nar kabuğu · Antioksidan kapasite · Fenolik bileşenler

Abstract

The aim of this study was to extract bioactive compounds from pomegranate peels (Punica granatum L.) using the ultrasound-assisted extraction (UDE) method within a zero-waste approach and to investigate the effects of different extraction conditions on total phenolic content and antioxidant capacity. Fresh pomegranate peels were separated into inner and outer layers, and extractions were performed using 80% methanol as a solvent under different ultrasound power levels (50% and 100%) and durations (5, 10, and 20 minutes). The results showed that the outer peel exhibited the highest total phenolic content, reaching 58.73 ± 0.79 mg GAE/g DM under 100% power and 10 minutes of extraction. The maximum antioxidant capacity was observed in outer peel extracts at 3177.75 ± 6.06 ppm TEAC/g DM with 100% power and 20 minutes. In the inner peel, the highest total phenolic content was found as 7.31 ± 0.03 mg GAE/g DM. FTIR and SEM analyses demonstrated that the structural integrity of the pomegranate peel was preserved while facilitating the release of active compounds. These findings suggest that pomegranate peels possess significant potential for valorization through environmentally friendly processes, offering opportunities for the development of value-added products in the food and pharmaceutical industries.

Özet

Bu çalışmanın amacı, sıfır atık yaklaşımıyla nar kabuklarından (Punica granatum L.) biyoaktif bileşenlerin ultrason destekli ekstraksiyon (UDE) yöntemi kullanılarak elde edilmesi ve farklı ekstraksiyon koşullarının fenolik madde içeriği ile antioksidan kapasite üzerindeki etkilerinin belirlenmesidir. Taze nar kabuklarının iç ve dış kısımları ayrı ayrı değerlendirilmiş, %80’lik metanol çözgeni ile farklı ultrasonik güç (%50 ve %100) ve süre (5, 10 ve 20 dakika) kombinasyonlarında ekstraksiyon gerçekleştirilmiştir. Elde edilen sonuçlara göre, dış kabuğun toplam fenolik madde içeriği %100 güç ve 10 dakika ekstraksiyon süresinde maksimum 58.73 ± 0.79 mg GAE/g KM değerine ulaşmıştır. En yüksek antioksidan kapasite ise dış kabuk örneklerinde %100 güç ve 20 dakikada 3177.75 ± 6.06 ppm TEAC/g KM olarak ölçülmüştür. İç zar örneklerinde ise en yüksek fenolik madde miktarı 7.31 ± 0.03 mg GAE/g KM değerinde bulunmuştur. FTIR ve SEM analizleri, nar kabuğunun yapısal bütünlüğünü koruyarak aktif bileşenlerin salımını kolaylaştırdığını göstermiştir. Bu bulgular, nar kabuklarının çevre dostu yaklaşımlarla değerlendirilerek gıda ve ilaç sanayilerinde katma değerli ürünler elde edilmesi için önemli bir potansiyele sahip olduğunu ortaya koymaktadır.

🔖

Cite This Article

Özdere, B., Ata, Ö., Kul, Ş. M., Berk, B. B., Tavman, Ş., Kumcuoğlu, S. (2025). Extraction of Phenolic Compounds from Pomegranate Peels by Ultrasound Assisted Extraction and Determination of Antioxidant Activities. Akademik Gıda, Cilt: 23 Sayı: 1, 47–59. https://doi.org/10.24323/akademik-gida.1697264

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (41)

  1. Qu, W., Pan, Z., Ma, H. (2010). Extraction modeling and activities of antioxidants from pomegranate marc. Journal of Food Engineering, 99(1), 16-23.
  2. Caruso, A., Barbarossa, A., Tassone, A., Ceramella, J., Carocci, A., Catalano, A., Basile, G., Fazio, A., Iacopetta, D., Franchini, C., Sinicropi, M.S. (2020). Pomegranate: Nutraceutical with promising benefits on human health. Applied Sciences, 10(19), 6915.
  3. Akhtar, S., Ismail, T., Fraternale, D., Sestili, P. (2015). Pomegranate peel and peel extracts: Chemistry and food features. Food Chemistry, 174, 417-425.
  4. Sharayei, P., Azarpazhooh, E., Zomorodi, S., Ramaswamy, H.S. (2019). Ultrasound assisted extraction of bioactive compounds from pomegranate (Punica granatum L.) peel. LWT-Food Science and Technology, 101, 342-350.
  5. Demir, T., Akpınar, Ö., Kara, H., Güngör, H. (2019). Nar kabuğundan antimikrobiyal ve antioksidan aktiviteye sahip fenolik bileşiklerin ekstraksiyon koşullarinin optimizasyonu. Gıda, 44(2), 369-382.
  6. Azmir, J., Zaidul, I.S.M., Rahman, M.M., Sharif, K.M., Mohamed, A., Sahena, F., Jahurul, M.H.A., Ghafoor, K., Norulaini, N.A.N., Omar, A.K.M. (2013). Techniques for extraction of bioactive compounds from plant materials: A review. Journal of Food Engineering, 117(4), 426-436.
  7. Li, Y., Guo, C., Yang, J., Wei, J., Xu, J., Cheng, S. (2006). Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chemistry, 96(2), 254-260.
  8. Ghafoor, K., Park, J., Choi, Y.H. (2010). Optimization of supercritical fluid extraction of bioactive compounds from grape (Vitis labrusca B.) peel by using response surface methodology. Innovative Food Science & Emerging Technologies, 11(3), 485-490.
  9. Mason, T.J., Chemat, F., Vinatoru, M. (2011). The extraction of natural products using ultrasound or microwaves. Current Organic Chemistry, 15(2), 237-247.
  10. Pan, Z., Qu, W., Ma, H., Atungulu, G.G., McHugh, T.H. (2012). Continuous and pulsed ultrasound-assisted extractions of antioxidants from pomegranate peel. Ultrasonics Sonochemistry, 19(2), 365-372.
  11. Kaderides, K., Goula, A.M., Adamopoulos, K.G. (2015). A process for turning pomegranate peels into a valuable food ingredient using ultrasound-assisted extraction and encapsulation. Innovative Food Science & Emerging Technologies, 31, 204-215.
  12. Vilkhu, K., Mawson, R., Simons, L., Bates, D. (2008). Applications and opportunities for ultrasound assisted extraction in the food industry-A review. Innovative Food Science & Emerging Technologies, 9(2), 161-169.
  13. Mphahlele, R.R., Fawole, O.A., Makunga, N.P., Opara, U. L. (2016). Effect of drying on the bioactive compounds, antioxidant, antibacterial and antityrosinase activities of pomegranate peel. BMC complementary and alternative medicine, 16, 1-12.
  14. Official Methods of Analysis, Association of Official Analytical Chemist. Gaithersburg: 2005.
  15. Singleton, V.L. (1999). Lamuela-Raventos: Analysis of total phenoles and other oxidation substartes and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299, 152.
  16. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237.
  17. Al-Rawahi, A.S., Rahman, M.S., Guizani, N., Essa, M.M. (2013). Chemical composition, water sorption isotherm, and phenolic contents in fresh and dried pomegranate peels. Drying Technology, 31(3), 257-263.
  18. Chen, X.D., Mujumdar, A.S. (Eds.) (2009). Drying technologies in food processing. John Wiley & Sons.
  19. Galaz, P., Valdenegro, M., Ramírez, C., Nuñez, H., Almonacid, S., Simpson, R. (2017). Effect of drum drying temperature on drying kinetic and polyphenol contents in pomegranate peel. Journal of Food Engineering, 208, 19-27.
  20. Derakhshan, Z., Ferrante, M., Tadi, M., Ansari, F., Heydari, A., Hosseini, M. S., Sadrabad, E. K. (2018). Antioxidant activity and total phenolic content of ethanolic extract of pomegranate peels, juice and seeds. Food and Chemical Toxicology, 114, 108-111.
  21. El-Hamamsy, S.M.A., El-Khamissi, H.A.Z. (2020). Phytochemicals, antioxidant activity and identification of phenolic compounds by HPLC of pomegranate (Punica granatum L.) peel extracts. Journal of Agricultural Chemistry and Biotechnology, 11(4), 79-84.
  22. Campos, L., Seixas, L., Henriques, M.H., Peres, A. M., Veloso, A.C. (2022). Pomegranate Peels and Seeds as a Source of Phenolic Compounds: Effect of Cultivar, By‐Product, and Extraction Solvent. International Journal of Food Science, 2022(1), 9189575.
  23. Suleria, H.A., Barrow, C.J., Dunshea, F.R. (2020). Screening and characterization of phenolic compounds and their antioxidant capacity in different fruit peels. Foods, 9(9), 1206.
  24. Derakhshan, Z., Ferrante, M., Tadi, M., Ansari, F., Heydari, A., Hosseini, M.S., Conti, G.O., Sadrabad, E. K. (2018). Antioxidant activity and total phenolic content of ethanolic extract of pomegranate peels, juice and seeds. Food and Chemical Toxicology, 114, 108-111.
  25. Yan, L., Zhou, X., Shi, L., Shalimu, D., Ma, C., Liu, Y. (2017). Phenolic profiles and antioxidant activities of six Chinese pomegranate (Punica granatum L.) cultivars. International journal of food properties, 20(sup1), S94-S107.
  26. Elfalleh, W., Hannachi, H., Tlili, N., Yahia, Y., Nasri, N., Ferchichi, A. (2012). Total phenolic contents and antioxidant activities of pomegranate peel, seed, leaf and flower. Journal of Medicinal Plants Research, 6(32), 4724-4730.
  27. Safdar, M., Naqvi, S.A., Anjum, F., Pasha, I., Shahid, M., Waliullah, Jaskani, M.J., Khan, I.A., Aadil, R.M. (2021). Microbial biofilm inhibition, antioxidants, and chemical fingerprints of Afghani pomegranate peel extract documented by gas chromatography–mass spectrometry and Fourier transformation infrared. Journal of Food Processing and Preservation, 45(7), e15657.
  28. Heredia-Guerrero, J.A., Benítez, J.J., Domínguez, E., Bayer, I.S., Cingolani, R., Athanassiou, A., Heredia, A. (2014). Infrared and Raman spectroscopic features of plant cuticles: a review. Frontiers in Plant Science, 5, 305.
  29. Hu, Y., Qian, W., Fan, S., Yang, Y., Liao, H., Zhuang, G., Gao, S. (2024). Ultrasonic-assisted extraction of phenolic compounds from Lonicera similis flowers at three harvest periods: Comparison of composition, characterization, and antioxidant activity. Molecules, 29(14), 3280.
  30. Altemimi, A., Watson, D. G., Choudhary, R., Dasari, M.R., Lightfoot, D.A. (2016). Ultrasound assisted extraction of phenolic compounds from peaches and pumpkins. Plos One, 11(2), e0148758.
  31. Soltanzadeh, M., Peighambardoust, S.H., Ghanbarzadeh, B., Mohammadi, M., Lorenzo, J.M. (2021). Chitosan nanoparticles as a promising nanomaterial for encapsulation of pomegranate (Punica granatum L.) peel extract as a natural source of antioxidants. Nanomaterials, 11(6), 1439.
  32. Jabrail, F.H., Hameed, A. R., Majdoub, H. (2023). Effects of Surface Morphology and Type of Cross-linking of Chitosan-Pectin Microspheres on their Degree of Swelling and Favipiravir Release Behavior.
  33. Safdar, M., Naqvi, S.A., Anjum, F., Pasha, I., Shahid, M., Waliullah, Jaskani, M.J., Khan, I.A., Aadil, R.M. (2021). Microbial biofilm inhibition, antioxidants, and chemical fingerprints of Afghani pomegranate peel extract documented by gas chromatography–mass spectrometry and Fourier transformation infrared. Journal of Food Processing and Preservation, 45(7), e15657.
  34. Dias, R.F. (2005). Spectroscopy: How do we know what organic molecules look like. Organic Chemistry (CHEM311) fall, 48-58.
  35. Ben-Ali, S., Akermi, A., Mabrouk, M., Ouederni, A. (2018). Optimization of extraction process and chemical characterization of pomegranate peel extract. Chemical Papers, 72(8), 2087-2100.
  36. Siddiqui, V.U., Almehjani, T.M., Sapuan, S.M., Jamal, T., Ilyas, R. A., Eldin, S.M., Khan, A., Jameel, Y. (2024). Isolation and characterization of cellulose from pomegranate (Punica granatum) peel. Journal of Natural Fibers, 21(1), 2299943.
  37. Emam‐Djomeh, Z., Moghaddam, A., Yasini Ardakani, S.A. (2015). Antimicrobial activity of pomegranate (Punica granatum L.) peel extract, physical, mechanical, barrier and antimicrobial properties of pomegranate peel extract‐incorporated sodium caseinate film and application in packaging for ground beef. Packaging Technology and Science, 28(10), 869-881.
  38. Ali, A., Chen, Y., Liu, H., Yu, L., Baloch, Z., Khalid, S., Zhu, J., Chen, L. (2019). Starch-based antimicrobial films functionalized by pomegranate peel. International journal of biological macromolecules, 129, 1120-1126. Zhong, C., Zu, Y., Zhao, X., Li, Y., Ge, Y., Wu, W., Zhang, Y., Li, Y., Guo, D. (2016). Effect of superfine grinding on physicochemical and antioxidant properties of pomegranate peel. International Journal of Food Science & Technology, 51(1), 212-221.
  39. Elnjikkal Jerome, R., Dwivedi, M. (2022). Microwave vacuum drying of pomegranate peel: Evaluation of specific energy consumption and quality attributes by response surface methodology and artificial neural network. Journal of Food Processing and Preservation, 46(3), e16325.
  40. Ali, A., Chen, Y., Liu, H., Yu, L., Baloch, Z., Khalid, S., Zhu, J., Chen, L. (2019). Starch-based antimicrobial films functionalized by pomegranate peel. International journal of biological macromolecules, 129, 1120-1126.
  41. Elnjikkal Jerome, R., Dwivedi, M. (2022). Microwave vacuum drying of pomegranate peel: Evaluation of specific energy consumption and quality attributes by response surface methodology and artificial neural network. Journal of Food Processing and Preservation, 46(3), e16325.