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Journals / Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi / 2025 / Cilt: 25 Sayı: 6

Effect of Titanium Dioxide Nanoparticle Dose and Photoperiod on Oxidative Stress and Growth of Chlorella vulgaris Microalgae

Chlorella vulgaris Mikroalginin Oksidatif Stres ve Büyümesi Üzerine Titanyum Dioksit Nanopartikül Dozu ve Fotoperiyotun Etkisi

Nazire Pınar Tanattı(SAKARYA UYGULAMALI BİLİMLER ÜNİVERSİTESİ)0000-0002-2904-7334
Meryem Aksu(SAKARYA ÜNİVERSİTESİ)0000-0003-0678-7150
Gamze Katircioğlu Sinmaz(SAKARYA ÜNİVERSİTESİ)0000-0003-2416-2680
Büşra Erden(SAKARYA ÜNİVERSİTESİ)0000-0002-4519-2531
Ahsen Akbulut Uludağ(SAKARYA ÜNİVERSİTESİ)0000-0002-1320-5975
Füsun Boysan(SAKARYA ÜNİVERSİTESİ)0000-0003-2100-0264
İsmail Ayhan Şengil(SAKARYA ÜNİVERSİTESİ)0000-0002-1858-3369
Pages
1261–1271
Publication Date
2025-12-03
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study contributes to the understanding of the complex interactions between the metabolic processes of C. vulgaris at different n.TiO2 particle doses and different photoperiods, and to the understanding of microalgae responses in nanoparticle-based environments and their potential applications in sustainable biotechnology.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, the effects of TiO2 nanoparticles on various biochemical and physiological growth of Chlorella vulgaris microalgae were investigated.
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Key Findings & Data:The effects of different n.TiO2 concentrations (2.5, 5, 10, 25, 50, 75 mg/L) and different photoperiods (6L:1DK, 12L:12D, 18L:6D and 24L:0D) on SOD and APX antioxidant enzyme activities, MDA and H2O2 amounts, Chl-a and Chl-b pigments, TSS and lipid were evaluated.
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Conclusion & Impact:This study contributes to the understanding of the complex interactions between the metabolic processes of C. vulgaris at different n.TiO2 particle doses and different photoperiods, and to the understanding of microalgae responses in nanoparticle-based environments and their potential applications in sustainable biotechnology.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:n.TiO2 · antioksidan enzim aktivitesi · MDA

Abstract

In this study, the effects of TiO2 nanoparticles on various biochemical and physiological growth of Chlorella vulgaris microalgae were investigated. The effects of different n.TiO2 concentrations (2.5, 5, 10, 25, 50, 75 mg/L) and different photoperiods (6L:1DK, 12L:12D, 18L:6D and 24L:0D) on SOD and APX antioxidant enzyme activities, MDA and H2O2 amounts, Chl-a and Chl-b pigments, TSS and lipid were evaluated. While an increase in SOD and MDA amounts was observed in the control group at high n.TiO2 doses, a decrease in APOD and H2O2 amounts was observed. It was determined that oxidative stress occurred in C. ulgari microalgae cells exposed to high nanoparticle concentrations. Chl-a, Chl-b and TSS parameters varied depending on the n.TiO2 dose and increased between 10 and 50 mg/L n.TiO2 doses. The change in lipid amount was found to decrease at low n.TiO2 doses. It was observed that oxidative stress increased at different photoperiods, while toxic effects were partially reduced at 12L:12D photoperiod. Changes in MDA and H2O2 amounts were determined at 12L:12D and 18L:6D photoperiods. Significant changes in chlorophyll pigments, TSS and lipid amounts compared to the control group were observed as significant increases at 12L:12D and 24L:0D photoperiods. This study contributes to the understanding of the complex interactions between the metabolic processes of C. vulgaris at different n.TiO2 particle doses and different photoperiods, and to the understanding of microalgae responses in nanoparticle-based environments and their potential applications in sustainable biotechnology.

Özet

Bu çalışmada, TiO2 nanopartikülünün Chlorella vulgaris mikroalgindeki çeşitli biyokimyasal ve fizyolojik büyümesi üzerine etkileri incelenmiştir. Farklı n.TiO2 konsantrasyonlarının (2,5, 5, 10, 25, 50, 75 mg/L) ve farklı fotoperiyodun (6A:18K, 12A:12K, 18A:6K ve 24A:0K) SOD ve APOD antioksidan enzim aktiviteleri, MDA ve H2O2 miktarları, Chl-a ve Chl-b pigmentleri, AKM ve lipid üzerindeki etkisi değerlendirilmiştir. Yüksek n.TiO2 dozlarında kontrol grubuna SOD ve MDA miktarında artış gözlemlenirken APOD ve H2O2 miktarlarında azalma gözlemlenmiştir. Yüksek nanopartikül konsantrasyonlarına maruz kalan C. vulgaris mikroalg hücrelerinde oksidatif stres oluştuğu belirlenmiştir. Chl-a, Chl-b ve AKM parametreleri n.TiO2 dozuna bağlı olarak değişkenlik göstermekte olup 10 ve 50 mg/L n.TiO2 dozları aralığında artış göstermiştir. Lipid miktarındaki değişim ise düşük n.TiO2 dozlarında azalma eğilimde olduğu bulunmuştur. Farklı fotoperiyotlarda oksidatif stresin arttığı, 12A:12K fotoperiyotta ise toksik etkileri kısmen azaldığı gözlemlenmiştir. MDA ve H2O2 miktarılarındaki değişim 12A:12K ve 18A:6K fotoperiyorlarında olduğu belirlenmiştir. Klorofil pigmentleri, AKM ve lipid miktarında kontrol grubuna göre anlamlı değişim 12A:12K ve 24A:0K fotoperiyotta belirgin artış şeklinde olmaktadır. Farklı n.TiO2 partikül dozlarının ve farklı fotoperiyotların C. vulgaris' in metabolik süreçleri arasındaki karmaşık etkileşimlere değerli sonuçlar sunarak, nanopartikül tabanlı ortamlardaki mikroalg tepkilerinin ve sürdürülebilir biyoteknolojideki potansiyel uygulamalarının anlaşılmasına bu çalışma ile katkıda bulunmaktadır.

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Cite This Article

Tanattı, N. P., Aksu, M., Sinmaz, G. K., Erden, B., Uludağ, A. A., Boysan, F., Şengil, İ. A. (2025). Effect of Titanium Dioxide Nanoparticle Dose and Photoperiod on Oxidative Stress and Growth of Chlorella vulgaris Microalgae. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, Cilt: 25 Sayı: 6, 1261–1271. https://doi.org/10.35414/akufemubid.1637251

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