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Journals / Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi / 2026 / Cilt: 26 Sayı: 1

Evaluation Of Pyrazoline-Bearing Primary Sulfonamides As Potent Inhibitors of Lactoperoxidase

Pirazolin Taşıyan Primer Sülfonamidlerin Potansiyel Laktoperoksidaz Inhibitörü Olarak Değerlendirilmesi

Nurcan Dedeoğlu(ADANA ALPARSLAN TURKES SCIENCE AND TECHNOLOGY UNIVERSITY)0000-0002-4008-2587
Ayşenur Demir(NISANTASI UNIVERSITY, HEALTH SERVICES VOCATIONAL SCHOOL, DEPARTMENT OF MEDICAL SERVICES AND TECHNIQUES, MEDICAL LABORATORY TECHNIQUES PR. (PAID))0009-0002-3115-4582
Cem Yamali(CUKUROVA UNIVERSITY, FACULTY OF PHARMACY, DEPARTMENT OF BASIC PHARMACEUTICAL SCIENCES)0000-0002-4833-7900
Semra Işık(Balıkesir University, Faculty of Arts and Science, Chemistry Department, Biochemistry Division)0000-0002-3022-0878
Pages
36–43
Publication Date
2026-01-19
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The IC50 value and Ki values for the most effective inhibitor, compound 3, were calculated as 0.144±0.03 and 6.06±4.06 µM, respectively, and it was found that this compound inhibited LPO enzyme activity non-competitively.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Lactoperoxidase (LPO E.C. 1.11.1.7) is a glycoprotein belonging to the class of oxidoreductases and constitutes a notable member of the peroxidase enzyme family.
📊
Key Findings & Data:In the current study, to examine the inhibitory effects of 8 different polyfluoro-substituted pyrazoline-type primary sulfonamide derivative compounds on LPO enzyme activity, LPO enzyme was extracted from buffalo milk and purified using Sepharose 4B-etilendiamin-4-tiyoüreido-benzensülfonamid affinity column, resulting in a 2212.45-fold purification, a 19.31 % yield, and a specific activity of 4955.9 EU/mg.
💡
Conclusion & Impact:The IC50 value and Ki values for the most effective inhibitor, compound 3, were calculated as 0.144±0.03 and 6.06±4.06 µM, respectively, and it was found that this compound inhibited LPO enzyme activity non-competitively.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Laktoperoksidaz · manda sütü · pirazolin

Abstract

Lactoperoxidase (LPO E.C. 1.11.1.7) is a glycoprotein belonging to the class of oxidoreductases and constitutes a notable member of the peroxidase enzyme family. The biological significance of LPO is attributed to its role as a natural defense system against microbial infection. In the current study, to examine the inhibitory effects of 8 different polyfluoro-substituted pyrazoline-type primary sulfonamide derivative compounds on LPO enzyme activity, LPO enzyme was extracted from buffalo milk and purified using Sepharose 4B-etilendiamin-4-tiyoüreido-benzensülfonamid affinity column, resulting in a 2212.45-fold purification, a 19.31 % yield, and a specific activity of 4955.9 EU/mg. Ki values for the active inhibitors were determined to be between 1.02±0.53-6.67±2.94 µM, while their IC50 values were in the range of 0.144±0.03-0.798±0.07 µM. The IC50 value and Ki values for the most effective inhibitor, compound 3, were calculated as 0.144±0.03 and 6.06±4.06 µM, respectively, and it was found that this compound inhibited LPO enzyme activity non-competitively.

Özet

Öz Laktoperoksidaz (LPO E.C. 1.11.1.7) peroksidaz enzim ailesinin önemli bir üyesi olan, oksidoredüktaz sınıfı bir glikoproteindir. LPO’ nun biyolojik önemi mikroorganizma istilasına karşı doğal bir koruma sistemi oluşturmasıdır. Bu çalışmada 8 farklı polifloro-sübstitüe pirazolin tipi sülfonamid türevi bileşiklerin etkilerini incelemek için öncelikle LPO enzimi manda sütünden Sepharose 4B-etilendiamin-4-tiyoüreido-benzensülfonamid afinite kolonu ile 19,31% verim ve 4955,9 EU/mg spesifik aktivite ile 2212,45 kat saflaştırılmıştır. LPO enzimi üzerine inhibisyon etkisi gösteren moleküllerin IC50 değerleri 0.144-0.798 µM, iken Ki değerleri 1.10-6.67 µM aralığında belirlenmiştir. En etkili inhibitör olan bileşik 3 için IC50 değeri ve Ki değerleri sırasıyla 0.144 ve 6.06 µM olarak hesaplanmıştır ve bu bileşiğin LPO enzim aktivitesini nonkompetitif olarak inhibe ettiği bulunmuştur.

🔖

Cite This Article

Dedeoğlu, N., Demir, A., Yamali, C., Işık, S. (2026). Evaluation Of Pyrazoline-Bearing Primary Sulfonamides As Potent Inhibitors of Lactoperoxidase. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, Cilt: 26 Sayı: 1, 36–43. https://doi.org/10.35414/akufemubid.1699085

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