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Journals / Ziraat Fakültesi Dergisi / 2024 / Cilt: 19 Sayı: 2

Determination of The Physicochemical, Functional and Sensory Properties of Kombucha Beverage Produced with Isparta Rose

Isparta Gülü ile Üretilen Kombucha İçeceğinin Fizikokimyasal, Fonksiyonel ve Duyusal Özelliklerinin Belirlenmesi

Nimet Çakıroğlu(SÜLEYMAN DEMİREL ÜNİVERSİTESİ)0000-0002-4743-7619
Kader Oral(SÜLEYMAN DEMİREL ÜNİVERSİTESİ)0009-0008-8105-5690
Bilge Ertekin Filiz(SÜLEYMAN DEMİREL ÜNİVERSİTESİ)0000-0002-5633-6641
Pages
105–118
Publication Date
2024-12-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result of the study, it was found that Isparta rose petals can be successfully used in Kombucha production as a product with high sensory acceptability.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Kombucha is a beverage produced by fermenting an solution containing black tea and sugar, and its health benefits have been emphasised.
📊
Key Findings & Data:Meanwhile, the biomass increase of Kombucha biofilms used as culture in the fermentation was determined.
💡
Conclusion & Impact:As a result of the study, it was found that Isparta rose petals can be successfully used in Kombucha production as a product with high sensory acceptability.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Kombucha · Isparta gülü · Fenolik bileşen

Abstract

Kombucha is a beverage produced by fermenting an solution containing black tea and sugar, and its health benefits have been emphasised. In this study, a functional drink (GK) was developed using kombucha culture and rose petals (Rosa damascena Mill). In addition, a black tea Kombucha drink (CK) was produced as control sample. The physicochemical, microbiological, sensory, antioxidant (ORAC-TEAC), phenolic and organic acid contents of the beverages were analysed. Meanwhile, the biomass increase of Kombucha biofilms used as culture in the fermentation was determined. The biomass increase was determined to be 105% in the black tea medium. The number of lactobacilli 4.11-5.75; lactococci 3.06-6.49; yeast 4.42-5.04; acetic acid bacteria 3.69-4.51 log cfu/mL were determined in the samples. The total phenolic content was determined to be 405 mg GAE/L in the CK sample and 123 mg GAE/L in the GK sample. The ORAC and TEAC values were found to be 9.00 and 2.53 µmol TE/mL higher in the CK sample (p<0.05). Among the organic acids, acetic acid was found to be the dominant acid, while oxalic, malic and lactic acids were also found in the samples. Among the phenolic compounds, gallic acid, chlorogenic acid and gallocatechin were found in the samples. The amount of caffeine in the CK sample was 35.48 mg/L. In the sensory evaluation, the GK sample was more appreciated by the panelists. As a result of the study, it was found that Isparta rose petals can be successfully used in Kombucha production as a product with high sensory acceptability.

Özet

Kombucha, siyah çay ve şeker içeren sulu çözeltinin fermente edilmesi ile üretilen, sağlık üzerine olumlu etkileri üzerinde durulan bir içecektir. Bu çalışmada Kombucha kültürü ve gül yaprakları (Rosa damascena Mill) kullanılarak fonksiyonel bir içecek (GK) geliştirilmiştir. Ayrıca kontrol örneği olarak siyah çaylı kombucha içeceğinin (CK) üretimi yapılmıştır. Üretilen içeceklerin fizikokimyasal özellikleri mikrobiyolojik, duyusal, antioksidan özellikleri (ORAC-TEAC), fenolik içeriği ve organik asit içeriği incelenmiştir. Aynı zamanda fermantasyonda kültür olarak kullanılan kombucha biyofilmlerinin biyokütle artışı belirlenmiştir. Biyokütle artışı siyah çaylı fermantasyon ortamında %105 olarak belirlenmiştir. Örneklerde laktobasil sayısı 4.11-5.75; laktokok sayısı 3.06-6.49; maya sayısı 4.42-5.04; asetik asit bakteri sayısı 3.69-4.51 log kob/mL aralıklarında belirlenmiştir. Toplam fenolik madde miktarları CK örneğinde 405 mg GAE/L ve GK örneğinde 123 mg GAE/L olarak tespit edilmiştir. ORAC ve TEAC değerleri CK örneğinde 9.00 ve 2.53 µmol TE/mL olarak daha yüksek bulunmuştur (p<0.05). Örneklerde organik asitlerden asetik asit baskın asit olarak tespit edilirken, yanı sıra oksalik, malik, ve laktik asitler bulunmuştur. Fenolik bileşenlerden gallik asit, klorojenik asit, gallokateşin örneklerde tespit edilmiştir. CK örneğinde kafein miktarı 35.48 mg/L olarak belirlenmiştir. Duyusal değerlendirmede GK örneği, panelistler tarafından daha fazla beğeni almıştır. Çalışmanın sonucunda, Isparta gül yapraklarının kombucha üretiminde, duyusal açıdan kabul edilebilirliği yüksek bir ürün olarak başarıyla kullanılabileceği bulunmuştur.

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Cite This Article

Çakıroğlu, N., Oral, K., Filiz, B. E. (2024). Determination of The Physicochemical, Functional and Sensory Properties of Kombucha Beverage Produced with Isparta Rose. Ziraat Fakültesi Dergisi, Cilt: 19 Sayı: 2, 105–118. https://doi.org/10.54975/sduzfd.1597516

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References (64)

  1. Akarca, G. & Tomar, O. (2020). Kırmızı ve mor sebzelerle hazırlanan kombucha çaylarının kalite özelliklerinin belirlenmesi. Mediterranean Agricultural Sciences, 33, 215–222. https://doi.org/10.29136/mediterranean.680360
  2. Akhavan, H. R., & Mehrizi, R. Z. (2016). Effects of Damask Rose (Rosa damascena Mill.) extract on chemical, microbial, and sensory properties of Sohan (an Iranian Confection) during storage. Journal of Food Quality & Hazards Control, 3(3).
  3. Andarini, F. R., Angkasa, D., Noviati, A., Melani, V., & Ronitawati, P. (2020). Development of high antioxidant yoghurt made from a mixture of Cashew (Anacardium occidentale) extract and red roses (Rosa damascena) juice. In 1st International Conference on Health.
  4. Anonim (1997). IDF 149A. Dairy Starter Cultures of Lactic Acid Bacteria (LAB), Brussels, Belgium.
  5. Ayed, L.A, Salwa, B. & Hamdi, M. (2017). Development of a beverage from red grape juice fermented with the Kombucha consortium. Annals of Microbiology, 67, 111–121. https://doi.org/10.1007/s13213-016-1242-2
  6. Baydar, H & Göktürk Baydar, N. (2017). Essential oils and phenolic compounds, antiradical and antioxidant activities of distillation products in oil-bearing rose ( Rosa damascena Mill.) Journal of Agricultural Sciences, 23, 1–9.
  7. Chochkov, R., Denkova, R., Denkova, Z., Denev, P., Vasileva, I., Dessev, T.,. & Slavov, A. (2022). Utilization of industrial Rosa damascena Mill. by-products and cocoa pod husks as natural preservatives in muffins. Periodica Polytechnica Chemical Engineering, 66(1), 157-166.
  8. Chu, S.C. & Chen, C. (2006). Effects of origins and fermentation time on the antioxidant activities of kombucha. Food Chemistry, 98(3), 502–507. https://doi.org/10.1016/j.foodchem.2005.05.080
  9. Dave, R. I., & Shah, N. P. (1996). Evaluation of media for selective enumeration of Streptococcus thermophilus, Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus, and bifidobacteria. Journal of Dairy Science, 79(9), 1529-1536.
  10. Değirmencioğlu, N., Yıldız, E., Şahan, Y., Güldaş, Metin & Gürbüz, O. (2019). Fermentasyon süresinin kombu çayı mikrobiyotası ve canlılık oranları üzerine etkileri. Akademik Gıda, 17(2), 200–211. https://doi.org/10.24323/akademik-gida.613567
  11. Dimidi, E Cox, S.R. Rosii, M. & Whelan, K. (2019). Fermented Foods: Definitions and Characteristics, Gastrointestinal Health and Disease. Nutrients, 11(1806).
  12. Dinçoğlu, A. H., & Rugji, J. (2021). Use of rose oil in probiotic fermented whey as a functional food. Journal of Food Science and Technology, 58, 2705-2713.
  13. Dufresne, C. & Farnworth, E. (2000). Tea, Kombucha, and health: A review. Food Research International. https://doi.org/10.1016/S0963-9969(00)00067-3
  14. Ertürkmen, P., Bulantekin, Ö., & Alp-Baltakesmez, D. (2024) Investigation of the potential of Rosa damascena vinegar fermented with probiotic lactic acid bacteria as a functional food. Revista Mexicana de Ingeniería Química 23,1.Alim24156.
  15. Fu, Caili Yan, Fen Cao, Zeli Xie, Fanying & Lin, Juan. (2014). Antioxidant activities of kombucha prepared from three different substrates and changes in content of probiotics during storage. Food Science and Technology, 34(1), 123–126.
  16. Gaggia, Francesca Baffoni, Loredana Galiano, Michele Nielsen, Dennis Sandris Jakobsen, Rasmus Riemer Leonardo, Josue Bosi, Sara Truzzi, Francesca Musumeci, Federica Dinelli, Giovanni & Gioia, Diana Di. (2019). Kombucha beverage from green, black and rooibos teas: A Comparative study looking at microbiology, chemistry and antioxidant activity. Nutrients, 11(1), 1–22. https://doi.org/10.3390/nu11010001
  17. Giritlioğlu, N.,Yıldız, E. & Gürbüz, O. (2020). Kombu çayı üretiminde kapari tomurcuklarının (Capparis spp.) kullanımının fenolikler, antioksidant kapasite ve biyoerişilebilirliğe etkisi. Akademik Gıda, 18, 390–401. https://doi.org/10.24323/akademik-gida.850909
  18. Gökdoğan, O. (2013). Isparta yöresinde yağ gülü yetiştiriciliğinin türkiye ekonomisindeki yeri. Journal of Süleyman Demirel University Institute of Social Sciences Year, 1(Special), 51–58.
  19. Gül, H., & Tekeli, S.G. (2019). Inclusion of Rosa damascena Mill. powder into cookies: nutritional, antioxidant and quality characteristics. International Journal of Agriculture Forestry and Life Sciences, 3(2), 301-306.
  20. Gül, H., Acun, S., Hayıt, F., & Şirikçi, B.S. (2021). Geleneksel Ekşi Mayalı Isparta ekmeğinin bazı kalite karakteristikleri açısından değerlendirilmesi. Ziraat Fakültesi Dergisi, 16(1), 34-45.
  21. Gül, E. E., Biçici, A. U., Gül, H., & Acun, S. (2024). Çölyak hastaları için ısparta gülü (rosa damascena mıll.) İle zenginleştirilmiş glutensiz dondurma külahı geliştirilmesi. Mühendislik Bilimleri ve Tasarım Dergisi, 12(2), 345-356.
  22. Gülboy, E. N. (2024). Lavanta ve gül yağı ilavesinin dondurmanın bazı kimyasal ve duyusal kalite kriterlerine etkisi.Yüksek Lisans Tezi. Selçuk Üniversitesi, Sağlık Bilimleri Enstitüsü, 85 s.
  23. Güldane, M., Bayram, M., Topuz, S., Kaya, C., Gök, H.B., Bülbül, M., Koç, M. (2017). Beyaz, siyah ve yeşil çay kullanılarak üretilen kombuchaların bazı özelliklerinin belirlenmesi determination of some properties in kombucha produced with white, black and green tea. Gaziosmanpaşa Üniversiresi Ziraat Fakültesi Dergisi, 34(1), 46–56. https://doi.org/10.13002/jafag1101
  24. Güneş, Şeyma Nur & Akcan, T. (2022). Yenilebilir çiçek olarak gülün önemi ve Osmanlı mutfak kültüründeki yeri. Aydın Gastronomy, 6(2), 325–334. https://doi.org/10.17932/IAU.GASTRONOMY.2017.016/gastronomy
  25. Halkman, A.K. 2005. Gıda Mikrobiyolojisi Uygulamaları. Başak Matbaacılık Ltd. Şti. 358.
  26. Ivanov, G., Dimitrova-Dicheva, M., Mihalev, K., & Ivanova, I. (2023). Viability of lactic acid bacteria in polyphenol-enriched fermented milks. In BIO Web of Conferences (Vol. 58, p. 01001). EDP Sciences.
  27. Jayabalan, R., Malbaša, R.V., Lončar, E.S. Vitas, J. S. & Sathishkumar, Muthuswamy. (2014). A review on kombucha tea-microbiology, composition, fermentation, beneficial effects, toxicity, and tea fungus. Comprehensive Reviews in Food Science and Food Safety, 13(4), 538–550. https://doi.org/10.1111/1541-4337.12073
  28. Kamali, Z., Moazzezi, S., & Labbeiki, G. (2020). An investigation on the possibility of production of cookie containing sunflower seed meal flour and Rosa damascena waste extract. Journal of Food and Bioprocess Engineering, 3(2), 147-159.
  29. Kandylis, Panagiotis Bekatorou, Argyro Dimitrellou, Dimitra Plioni, Iris & Giannopoulou, Kanella. (2021). Health promoting properties of cereal vinegars. Foods (Kd 10, Number 2, lk 1–30). MDPI AG. https://doi.org/10.3390/foods10020344
  30. Laavanya, D., Shirkole, S., & Balasubramanian, P. (2021). Current challenges, applications and future perspectives of SCOBY cellulose of Kombucha fermentation. Journal of Cleaner Production, 295, 126454.
  31. La Torre, C., Fazio, A., Caputo, P., Plastina, P., Caroleo, M. C., Cannataro, R., & Cione, E. (2021). Effects of long-term storage on radical scavenging properties and phenolic content of kombucha from black tea. Molecules, 26(18), 5474.
  32. Leroy, F & Vuyst, L.D. (2014). Fermented food in the context of a healthy diet: how to produce novel functional foods ? Current Opinion in Clinical Nutrition and Metabolic Care, 17(6), 574–581. https://doi.org/10.1097/MCO.0000000000000108
  33. Liamkaew, R., Chattrawanit, J & Danvirutai, P. (2016). Kombucha production by combinations of black tea and apple juice. Science and Technology RMUTT Journal, 6(2), 139–146.
  34. Malbaša, R., Lončar, E. Djurić, M. Klašnja, M. Kolarov, L.J. & Markov, S. (2006). Scale-up of black tea batch fermentation by Kombucha. Food and Bioproducts Processing. https://doi.org/10.1205/fbp.05061
  35. Nguyen, N. K., Dong, N.T. N., Nguyen, H. T. & Le, P.H. (2015). Lactic acid bacteria: promising supplements for enhancing the biological activities of kombucha. SpringerPlus, 4(1), 1–6. https://doi.org/10.1186/s40064-015-0872-3
  36. Onder, S., Erbas, S., Mutlucan, M. & Onder, D. (2022). Plant physiology and biochemistry investigation of phenological, primary and secondary metabolites changes during flower developmental of Rosa damascena. Plant Physiology and Biochemistry, 192(July), 20–34. https://doi.org/10.1016/j.plaphy.2022.09.032
  37. Özdemir, N., Kök-Taş, T., & Guzel-Seydim, Z. (2015). Effect of Gluconacetobacter spp. on kefir grains and kefir quality. Food Science and Biotechnology, 24, 99-106.
  38. Özdemir, N. & Budak, N.H. (2022). Bioactive compounds and volatile aroma compounds in rose ( Rosa damascena Mill.) vinegar during the aging period. Food Bioscience, 50(June), 102062. https://doi.org/10.1016/j.fbio.2022.102062
  39. Palamutoğlu, M. İ. & Baş, M. (2020). Traditional fermented foods of Turkey. Journal of Health Sciences and Research, 3(December), 200–220.
  40. Sanarico, D. Motta, S. Bertolini, L. & Antonelli, A. (2003). HPLC determination of organic acids in traditional balsamic vinegar of Reggio Emilia. Journal of Liquid Chromatography and Related Technologies, 26(13), 2177–2187. https://doi.org/10.1081/JLC-120022402
  41. Stanton, C., Ross, R.P., Fitzgerald, G. F. & Sinderen, D.V. (2005). Fermented functional foods based on probiotics and their biogenic metabolites. Current Opinion in Biotechnology, 198–203. https://doi.org/10.1016/j.copbio.2005.02.008
  42. Susilowati, A. A., Aspiyanto P.D L., Hakiki, M & Yati, M. (2017). Purification and components ıdentification on spinach ( Amaranthus sp. L.) and Broccoli ( Brassica oleracea L.) Fermented by kombucha culture as source of folic acid through stirred microfiltration cell ( SMFC ). IPTEK-Journal of Proceeding Series, International Seminar on Chemistry Proceeding, 76–88. http://dx.doi.org/10.12962/j23546026.y2017i4.3744
  43. Talebi, M.,Frink, L A., Patil, R.A. & Armstrong, D.W. (2017). Examination of the varied and changing ethanol content of commercial kombucha products. Food Analytical Methods, 10, 4062–4067. https://doi.org/10.1007/s12161-017-0980-5
  44. Tamer, C.E., Akpınar B., Özkan Karabacak, A., Durgut, S., Tunçkal, C., Bayramoğlu, G., Yolcı Ömeroğlu, P., Kamiloğlu Beştepe, S & Çopur, Ö U. (2022). Gıda sanayinde kombucha biyoselülozunun değerlendirilme olanakları. H. Gül &Hayıt F., Gıda Mühendisliği Alanında Yeni Yaklaşımlar (1. tr, lk 802 sayfa). İKSAD yayınevi.
  45. Tamer, C. E., Temel, S.G., Suna, S., Ozkan Karabacak, A., Ozcan, T., Yilmaz Ersan, L., Turkol Kaya, B. & Copur, O. U. (2021). Evaluation of bioaccessibility and functional properties of kombucha beverages fortified with different medicinal plant extracts. Turkish Journal of Agriculture and Forestry, 45(1), 13–32. https://doi.org/10.3906/tar-2003-75
  46. Tarhan, K. (2017). Kombucha çayı üretiminde farklı substrat kaynaklarının kullanımı. Yüksek Lisans Tezi, Akdeniz Üniversitesi. 128 sayfa.
  47. Ulusoy, A. (2019). Karayemiş (Prunus Laurocerasus), Siyah havuç (Daucus Carota L. Ssp. Sativus Var. Atrorubens Alef.), güvem (Prunus Spinosa) ve ahududu (Rubus İdaeus) kullanılarak üretilen kombucha çaylarının antioksidan aktivitelerininin araştırılması ve antosiyanin miktarının belirlenmesi. Yüksek Lisans Tezi. Uludağ Üniversitesi.
  48. Vázquez-Cabral, B.D., Rocha-Guzmán, N. E., Gallegos-Infante, J.A., González-Herrera, S. M., González-Laredo, R. F., Moreno-Jiménez, M. R. & Córdova-Moreno, I.TS. (2014). Chemical and sensory evaluation of a functional beverage obtained from infusions of oak leaves (Quercus resinosa) inoculated with the kombucha consortium under different processing conditions. Nutrafoods. 13, 169–178. https://doi.org/10.1007/s13749-014-0035-0
  49. Vinokur, Y., Rodov, V., Reznick, N., Goldman, G., Horev, B., Umiel, N. & Friedman, H., (2006). Rose petal tea as an antioxidant-rich beverage: Cultivar effects. Journal of Food Science, 71(1). https://doi.org/10.1111/j.1365-2621.2006.tb12404.x
  50. Vitas, J., Vukmanović, S., Čakarević, J., Popović, L. & Malbaša, R. (2020). Kombucha fermentation of six medicinal herbs: Chemical profile and biological activity. Chemical Industry and Chemical Engineering Quarterly, 26(2), 157–170. https://doi.org/10.2298/CICEQ190708034V
  51. Vitas, J. S., Cvetanovic, A. D., Maskovic, P. Z., Jaroslava, V Š & Malbasa, R.V. (2018). Chemical composition and biological activity of novel types of kombucha beverages with yarrow. Journal of Functional Foods, 44(February), 95–102. https://doi.org/10.1016/j.jff.2018.02.019
  52. Yavari, N., Mazaheri-Assadi, M., Mazhari, Z. H., Moghadam, M. B. & Larijani, K. (2018). Glucuronic acid rich kombucha-fermented pomegranate juice. Journal of Food Research, 7(1), 61–69. https://doi.org/10.5539/jfr.v7n1p61
  53. Zubaidah, E., Dewantari, F.J., Novitasari, F.R.,Srianta, I. & Blanc, P. J. (2018). Potential of snake fruit ( Salacca zalacca ( Gaerth.) Voss ) for the development of a beverage through fermentation with the Kombucha consortium. Biocatalysis and Agricultural Biotechnology, 13(September 2017), 198–203. https://doi.org/10.1016/j.bcab.2017.12.012
  54. Baydar, H & Göktürk Baydar, N. (2017). Essential oils and phenolic compounds, antiradical and antioxidant activities of distillation products in oil-bearing rose ( Rosa damascena Mill.) Journal of Agricultural Sciences, 23, 1–9.
  55. Chochkov, R., Denkova, R., Denkova, Z., Denev, P., Vasileva, I., Dessev, T.,. & Slavov, A. (2022). Utilization of industrial Rosa damascena Mill. by-products and cocoa pod husks as natural preservatives in muffins. Periodica Polytechnica Chemical Engineering, 66(1), 157-166.
  56. Değirmencioğlu, N., Yıldız, E., Şahan, Y., Güldaş, Metin & Gürbüz, O. (2019). Fermentasyon süresinin kombu çayı mikrobiyotası ve canlılık oranları üzerine etkileri. Akademik Gıda, 17(2), 200–211. https://doi.org/10.24323/akademik-gida.613567
  57. Dimidi, E Cox, S.R. Rosii, M. & Whelan, K. (2019). Fermented Foods: Definitions and Characteristics, Gastrointestinal Health and Disease. Nutrients, 11(1806).
  58. Güldane, M., Bayram, M., Topuz, S., Kaya, C., Gök, H.B., Bülbül, M., Koç, M. (2017). Beyaz, siyah ve yeşil çay kullanılarak üretilen kombuchaların bazı özelliklerinin belirlenmesi determination of some properties in kombucha produced with white, black and green tea. Gaziosmanpaşa Üniversiresi Ziraat Fakültesi Dergisi, 34(1), 46–56. https://doi.org/10.13002/jafag1101
  59. Leroy, F & Vuyst, L.D. (2014). Fermented food in the context of a healthy diet: how to produce novel functional foods ? Current Opinion in Clinical Nutrition and Metabolic Care, 17(6), 574–581. https://doi.org/10.1097/MCO.0000000000000108
  60. Onder, S., Erbas, S., Mutlucan, M. & Onder, D. (2022). Plant physiology and biochemistry investigation of phenological, primary and secondary metabolites changes during flower developmental of Rosa damascena. Plant Physiology and Biochemistry, 192(July), 20–34. https://doi.org/10.1016/j.plaphy.2022.09.032
  61. Özdemir, N. & Budak, N.H. (2022). Bioactive compounds and volatile aroma compounds in rose ( Rosa damascena Mill.) vinegar during the aging period. Food Bioscience, 50(June), 102062. https://doi.org/10.1016/j.fbio.2022.102062
  62. Susilowati, A. A., Aspiyanto P.D L., Hakiki, M & Yati, M. (2017). Purification and components ıdentification on spinach ( Amaranthus sp. L.) and Broccoli ( Brassica oleracea L.) Fermented by kombucha culture as source of folic acid through stirred microfiltration cell ( SMFC ). IPTEK-Journal of Proceeding Series, International Seminar on Chemistry Proceeding, 76–88. http://dx.doi.org/10.12962/j23546026.y2017i4.3744
  63. Tamer, C. E., Temel, S.G., Suna, S., Ozkan Karabacak, A., Ozcan, T., Yilmaz Ersan, L., Turkol Kaya, B. & Copur, O. U. (2021). Evaluation of bioaccessibility and functional properties of kombucha beverages fortified with different medicinal plant extracts. Turkish Journal of Agriculture and Forestry, 45(1), 13–32. https://doi.org/10.3906/tar-2003-75
  64. Zubaidah, E., Dewantari, F.J., Novitasari, F.R.,Srianta, I. & Blanc, P. J. (2018). Potential of snake fruit ( Salacca zalacca ( Gaerth.) Voss ) for the development of a beverage through fermentation with the Kombucha consortium. Biocatalysis and Agricultural Biotechnology, 13(September 2017), 198–203. https://doi.org/10.1016/j.bcab.2017.12.012