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Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 1996 / Cilt: 8 - Sayı: 4

Infectious Bovine Rhinotracheitis- Infectious Pustular Vulvovagınıtıs (IBR/IPV): Detection of Neutralization Antibodies and Isolation of the Virus ın MDBK and Primary Calf Kıdney Cell Cultures

Infeksiyöz Bovine Phinotracheitis - Infeksiyöz Pustular Vulvovaginitis (IBR/ IPV): Nötralizan Antikorlarının Saptanması ve MDBK, Primer Dana Böbrek Hücre Kültürlerinde Virus İzolasyonu

Özden KABAKLI
K. Tayfun ÇARLI
Pages
147–160
Publication Date
1996-12-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was found that IBR/IPV infection was present in all regions studied.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study 670 serum sampels obtained from cattle which seem to be healty, in various regions of Turkey were tested for IBR/IPV virus antibodies using macroneutralization test.
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Key Findings & Data:In addition, 5 nasal swab samples were used for virus isolation in MDBK and primary FDB cell cultures.
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Conclusion & Impact:It was found that IBR/IPV infection was present in all regions studied.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Infeksiyöz · Bovine · IBR/ IPV

Abstract

In this study 670 serum sampels obtained from cattle which seem to be healty, in various regions of Turkey were tested for IBR/IPV virus antibodies using macroneutralization test. In addition, 5 nasal swab samples were used for virus isolation in MDBK and primary FDB cell cultures. It was found that IBR/IPV infection was present in all regions studied. Three hundred fifty four (354) (%52.83) of 670 serum samples were found to have IBR/IPV virus neutralizing antibodies. Neutralizing dose 50(ND 50) values of the antibody carriers ranged betwwen 1:1.41 - 1:251. The virus was isolated from the nasal swab collected from a cow in primary FCK cell culture.

Özet

Bu çalışmada, Türkiye'nin çeşitli bölgelerinden, sağlıklı görülen sığırlarından toplanan 670 adet kan serumu makronötralizasyon testi ile IBR/IPV virus antikorları yönünden test edildi. Ayrıca klinik semptom gösteren 5 adet hayvandan burun Swab örnekleri alındı ve MDBK ve FDB hücre kültürlerinde virus izolasyonu amacıyla kullanıldı. Çalışılan bölgelerin tümünde infeksiyonun varlığı tespit edildi. Makro nötralizasyon testi ile kontrol edilen 670 adet kan serumundan 354 adedinin (%52.83) IBR/IPV virus nötralizan antikorları içerdiği belirlendi. Antikor taşıyıcılarının SN 50 , değerleri 1:1.41 - 1:251 arasında değişmekteydi . Virus İzolasyon amacıyla toplanan örnekler içinde bir inekten alınan burun swabından primer FDB hücre kültüründe virus izole edildi.

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Cite This Article

KABAKLI, Ö., ÇARLI, K. T. (1996). Infectious Bovine Rhinotracheitis- Infectious Pustular Vulvovagınıtıs (IBR/IPV): Detection of Neutralization Antibodies and Isolation of the Virus ın MDBK and Primary Calf Kıdney Cell Cultures. Etlik Veteriner Mikrobiyoloji Dergisi, Cilt: 8 - Sayı: 4, 147–160. https://ajindex.com/articles/132320

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References (8)

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