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Journals / Türk Doğa ve Fen Dergisi / 2026 / Cilt: 13 Sayı: 2

Optimization of Medium Components for Enhanced Expression of Recombinant Human Vascular Endothelial Growth Factor (VEGF165) in Kluyveromyces lactis GG799

Hülya Kuduğ Ceylan(Tokat Gaziosmanpaşa Üniversitesi)0000-0003-0365-2760
Cafer Meydan(EGE UNIVERSITY, FACULTY OF ENGINEERING, DEPARTMENT OF BIOENGINEERING, BIOENGINEERING PR.)0000-0001-6201-4860
Ayşe Nalbantsoy(EGE UNIVERSITY, FACULTY OF ENGINEERING, DEPARTMENT OF BIOENGINEERING, BIOENGINEERING PR.)0000-0002-9108-4451
Murat Elibol(EGE UNIVERSITY, FACULTY OF ENGINEERING, DEPARTMENT OF BIOENGINEERING, BIOENGINEERING PR.)0000-0002-6756-6290
Pages
95–105
Publication Date
2024-06-28
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results imply that the proposed optimized medium can serve as a model for a process development strategy, providing a foundation for obtaining elevated yields of other recombinant proteins in K. lactis.

📌 3 Key Takeaways

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Objective & Hypothesis:Vascular endothelial growth factor (VEGF165) serves as a vital biomarker, linked to diverse physiological processes and pathological conditions like cancer, owing to its pivotal role in angiogenesis.
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Key Findings & Data:Therefore, an efficient method to obtain recombinant VEGF165 in high yield is required.
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Conclusion & Impact:These results imply that the proposed optimized medium can serve as a model for a process development strategy, providing a foundation for obtaining elevated yields of other recombinant proteins in K. lactis.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Vasküler endotelyal büyüme faktörü (VEGF165) · Kluyveromyces lactis · Biyokütle optimizasyonu

Abstract

Vascular endothelial growth factor (VEGF165) serves as a vital biomarker, linked to diverse physiological processes and pathological conditions like cancer, owing to its pivotal role in angiogenesis. Therefore, an efficient method to obtain recombinant VEGF165 in high yield is required. This study focused on optimizing a medium to enhance the biomass of Kluyveromyces lactis (K. lactis) GG799 cells that express recombinant VEGF165. Through screening various media components, galactose, tryptone, and yeast extract were identified as the most effective components. Using the Box–Behnken design, the medium components were optimized, resulting in a notable 31% increase in biomass concentration. The optimized medium, comprising 45 g L-1 galactose, 30 g L-1 tryptone, and 5 g L-1yeast extract, achieved a biomass productivity (P) of 0.26 gDCW L-1 h-1. Additionally, the expression of VEGF165 reached 1024.09 μg per L of the culture medium, representing a 1.27-fold increase compared to the unoptimized medium. These results imply that the proposed optimized medium can serve as a model for a process development strategy, providing a foundation for obtaining elevated yields of other recombinant proteins in K. lactis.

Özet

Vasküler endotelyal büyüme faktörü (VEGF165), anjiyogenezdeki rolünden dolayı çeşitli fizyolojik süreçler ve kanser gibi patolojik hastalıklar ile ilişkili önemli bir biyobelirteçtir. Bu nedenle, yüksek verimde rekombinant VEGF165 elde etmek için etkili bir yöntem gereklidir. Bu çalışmada, Kluyveromyces lactis (K. lactis) GG799 hücrelerinde rekombinant VEGF165 ekspresyonunu artırmak amacıyla büyüme ortamının optimize edilmesi amaçlanmıştır. Farklı ortam bileşenlerinin taranması sonucunda galaktoz, tripton ve maya ekstraktı en etkili bileşenler olarak belirlenmiştir. Box-Behnken tasarımı kullanılarak seçilen ortam bileşenleri optimize edilmiş ve biyokütle konsantrasyonunda %31'lik kayda değer bir artış sağlanmıştır. 45 g L-1 galaktoz, 30 g L-1 tripton ve 5 g L-1 maya ekstraktı içeren optimize ortam ile 0,26 gDCW L-1 h-1 biyokütle üretkenliğine (P) ulaşılmıştır. Ayrıca VEGF165 ekspresyonu, optimize edilmemiş ortama kıyasla 1,27 kat artışla 1024,09 μg L-1 değerine ulaşmıştır. Elde edilen sonuçlar, önerilen optimize edilmiş ortamın K. lactis'te diğer rekombinant proteinlerin üretimine yönelik proses geliştirme stratejisi olarak bir model olabileceğini göstermektedir.

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Cite This Article

Ceylan, H. K., Meydan, C., Nalbantsoy, A., Elibol, M. (2024). Optimization of Medium Components for Enhanced Expression of Recombinant Human Vascular Endothelial Growth Factor (VEGF165) in Kluyveromyces lactis GG799. Türk Doğa ve Fen Dergisi, Cilt: 13 Sayı: 2, 95–105. https://doi.org/10.46810/tdfd.1474399

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