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Journals / Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi / 2026 / Cilt: 10 Sayı: 1

Determination of Antagonistic Activities of Endophytic Bacteria Isolated from Different Wheat Genotypes Against Fusarium culmorum

Farklı Buğday Genotiplerinden İzole Edilen Endofitik Bakterilerin Fusarium culmorum'a Karşı Antagonistik Aktivitelerinin Belirlenmesi

İnci Güler Güney(MARDİN ARTUKLU ÜNİVERSİTESİ, KIZILTEPE MESLEK YÜKSEKOKULU)0000-0002-2544-8712
Sibel Derviş(MARDİN ARTUKLU ÜNİVERSİTESİ, KIZILTEPE MESLEK YÜKSEKOKULU)0000-0002-4917-3813
Göksel Özer(BOLU ABANT İZZET BAYSAL ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, BİTKİ KORUMA BÖLÜMÜ)0000-0002-3385-2520
Hüsnü Aktaş(MARDİN ARTUKLU ÜNİVERSİTESİ, KIZILTEPE MESLEK YÜKSEKOKULU)0000-0001-6943-2109
Mehmet Ata Keske(MARDİN ARTUKLU ÜNİVERSİTESİ, FEN FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ)0000-0001-9219-927X
Pages
96–116
Publication Date
2024-04-29
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results demonstrated that the combination of S. maltophilia, Enterobacter sp., and B. subtilis (MY3+EY5+MM11) yielded the most favorable outcomes in terms of disease severity, plant height, wet weight, dry weight, root wet weight, and root dry weight.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study aimed to evaluate the physiological and biochemical properties and enzyme activities of endophytic bacteria obtained from different wheat genotypes, as well as their effectiveness against Fusarium culmorum, which causes root and crown rot in wheat.
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Key Findings & Data:The results obtained from double culture tests of isolates against F. culmorum showed that the inhibition rate varied between 80.56% and 13.90%.
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Conclusion & Impact:The results demonstrated that the combination of S. maltophilia, Enterobacter sp., and B. subtilis (MY3+EY5+MM11) yielded the most favorable outcomes in terms of disease severity, plant height, wet weight, dry weight, root wet weight, and root dry weight.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Endofit · Fusarium culmorum · biyolojik mücadele

Abstract

This study aimed to evaluate the physiological and biochemical properties and enzyme activities of endophytic bacteria obtained from different wheat genotypes, as well as their effectiveness against Fusarium culmorum, which causes root and crown rot in wheat. The results obtained from double culture tests of isolates against F. culmorum showed that the inhibition rate varied between 80.56% and 13.90%. The inhibition rate against F. culmorum was 80.59% for Bacillus subtilis (MM11), 69.41% for Stenotrophomonas maltophilia (EY5), and 61.10% for Enterobacter sp. (MY3) under in vitro conditions, the most effective isolates. Pseudomonas putida (EM9) and Pseudomonas orientalis (MM21) isolates gave positive results in all tests in the production of amylase, cellulase, phosphatase, ACC deaminase, and siderophore. To identify six promising isolates, 16S rRNA gene-based sequence analysis was utilized. The efficacy of bacterial strains against F. culmorum, pot experiments were conducted in a growth room (in vivo). The results demonstrated that the combination of S. maltophilia, Enterobacter sp., and B. subtilis (MY3+EY5+MM11) yielded the most favorable outcomes in terms of disease severity, plant height, wet weight, dry weight, root wet weight, and root dry weight. The combination of Stenotrophomonas rhizophila, P. putida, and P. orientalis (EY1+EM9+MM21) exhibited promising results. Utilizing effective bacterial strains is anticipated to reduce the dependence on and costs associated with chemical fertilizers and pesticides while minimizing their environmental impact. Furthermore, these strains show potential for commercial applications pending further validation procedures. The findings from this study significantly contribute to the field of biological control strategies against F. culmorum by leveraging the diverse capabilities of endophytic bacteria.

Özet

Bu araştırmada, farklı buğday genotiplerinden elde edilen endofitik bakterilerin, fizyolojik ve biyokimyasal özelliklerinin ile enzim aktivitelerinin yanısıra buğdayda kök ve kökboğazı çürüklüğüne neden olan Fusarium culmorum’a karşı etkinliğinin değerlendirilmesi amaçlanmıştır. İzolatların F. culmorum’a karşı ikili kültür testlerinden elde edilen sonuçlara göre inhibisyon oranı %80.56- %13.90 arasında değişiklik göstermiştir. In vitro koşullarda Fusarium culmorum’a karşı inhibisyon oranı %80.59 oranıyla Bacillus subtilis (MM11), %69.41 oranıyla Stenotrophomonas maltophilia (EY5) ve %61.10 oranıyla Enterobacter sp. (MY3) en etkili izolatlar olmuştur. Amilaz, selülaz, fosfataz, ACC deaminaz ve siderofor üretiminde Pseudomonas putida (EM9) ve Pseudomonas orientalis (MM21) izolatları tüm testte pozitif sonuç vermiştir. Umut vadeden altı izolat 16S rRNA geninin sekans analizi kullanılarak tanımlanmıştır. Bakteri suşlarının F. culmorum’a karşı etkinliğini değerlendirmek için iklim odası koşullarında (in vivo) saksı denemeleri kurulmuştur. Sonuçlar, Enterobacter sp., S. maltophilia ve B. subtilis (MY3+EY5+MM11) kombinasyonunun hastalık şiddeti, bitki boyu, yaş ağırlık, kuru ağırlık, kök yaş ağırlığı ve kök kuru ağırlığı açısından en uygun sonuçları verdiğini göstermiştir. Stenotrophomonas rhizophila, P. putida ve P. orientalis (EY1+EM9+MM21) kombinasyonu da oldukça etkili olmuştur. Etkili bakteri suşlarının kullanılmasının, çevresel etkilerini en aza indirirken, kimyasal gübre ve pestisitlere olan bağımlılığı ve bunlarla ilişkili maliyetleri azaltması beklenmektedir. Ek doğrulama prosedürlerinin ardından bu suşların, ticari uygulamalar için potansiyelinin olduğu düşünülmektedir. Bulgular, F. culmorum’a karşı çeşitli endofitik bakterilerin kullanıldığı biyolojik kontrol çalışmalarına katkıda bulunacaktır.

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Cite This Article

Güney, İ. G., Derviş, S., Özer, G., Aktaş, H., Keske, M. A. (2024). Determination of Antagonistic Activities of Endophytic Bacteria Isolated from Different Wheat Genotypes Against Fusarium culmorum. Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi, Cilt: 10 Sayı: 1, 96–116. https://doi.org/10.24180/ijaws.1386741

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