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Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 1990 / Cilt: 6 - Sayı: 6

A Study on the stocking of the suspended cell oultiıres in lorge volumes

Büyük Hacimde Süspanse Hücre Kültürlerinin Stoklanması Üzerinde Uygulamalar

S. İsmet GÜRHAN
Aysun ÖZTÜRKMEN
Tamer ERGÜÇ
Pages
99–104
Publication Date
1990-12-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

For that reason the experiment is realised in the gas phase of liquid nitrogen.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:For long term storage of so large quantities of cells it, ls necessary to keep them below minus 130·c.
📊
Key Findings & Data:The maximum capacity of the ampoules which are not demaged in minus 196·c is limited with 5 ml' To freeze the cells in at least 100 ml volume only polycarbonate ampaules are commercially available.
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Conclusion & Impact:For that reason the experiment is realised in the gas phase of liquid nitrogen.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Hücre Kültürü · Hücre Kültürünün Stoklanması · S. İsmet GÜRHAN

Abstract

For long term storage of so large quantities of cells it, ls necessary to keep them below minus 130·c. The maximum capacity of the ampoules which are not demaged in minus 196·c is limited with 5 ml' To freeze the cells in at least 100 ml volume only polycarbonate ampaules are commercially available. Those are resistant to steam sterilisation and freezing untill minus 156·c. For that reason the experiment is realised in the gas phase of liquid nitrogen.

Özet

Büyük hacimdaki hücrelerin uzun süreli muhafazası için -130·c nin altındaki sıcaklıklara ihtiyaç vardır. -196.C'ye dayanıklı hücre dondurma ampullerinin maksimum kapasitesi 5 ml'dir. 100 ml hacimdaki hücrelerin dondurularak muhafazası için ticarette sadece palikarbonat ampuller bulunmaktadır ki bunlar da ancak -150·c·ye kadar bozulmadan kalabilmektedirler. Bu nedenle denemeler sıvı azot gazında hücre muhafazası konusunda yoğunlaştırılmıştır. Bu çalışmada 15 seri hücre kültürü 100 ml hacimda dondurulmuştur. Bu hücrelerin çözdürüldükten sonraki üreyebilirlik oranının ortalama % 90 olduğu tespit edilmiştir.

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Cite This Article

GÜRHAN, S. İ., ÖZTÜRKMEN, A., ERGÜÇ, T. (1990). A Study on the stocking of the suspended cell oultiıres in lorge volumes. Etlik Veteriner Mikrobiyoloji Dergisi, Cilt: 6 - Sayı: 6, 99–104. https://ajindex.com/articles/130793

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References (6)

  1. CORIELL,.L. (1979):
  2. Preservation, Storage and Shipment. Methods in Enzymology, Vol. LVIII, eds. William B. Jakoby and Ira H. Pastan, Acd. Press.
  3. DOYLE, A., MORRIS, C.B., ARMITA:GE, W.J.: Cryopreservation of Animal Cells Upstream Processes: Equipment and Techniques, pp. 1-7, 1888 Alan R. liss, lnç. GRIFFITHS, J.B. Cell Biology: Experimental -Aspects, Animal Cell Biotechnology,eds. Ra/ E. Spier and J.B. Griffiths, vol. 1, ·1335 Acad Prsss. JENSEN, M. (1975): Storage of Large Volumes of Calls. Report of the Meeting of the Research Group of the Stand. Tech. Comm. Eur. Comm. Cont. FMD. Brescia./Padua, Sep. 1975.
  4. KASTEN, F.H. and: YIP; D.K.,: A Simple Device and Procedure for Succesful Freezing of Cells in Liquid Nitrogen Vapor. Methods in. Cell Bliology, ed. David M. PresGott, vol. XIV, 1976
  5. Acad Press.
  6. MAREL, P.,:Concentration. Animal •Cell Biotechnology Vol.2. eds. Ray E.Spier and J.B. Griffiths, 18.S5 Acad. Press. ·. SHARP, R.J. (1984): The preservation of Genetically Unstable Microorganisms and the Cryopreservation of Firmentation Seed Cultures in Advances in Biotechnological Proceses 3; eds. Alan R. Liss: Inc., 81-109., New York.