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Journals / Online Türk Sağlık Bilimleri Dergisi / 2024 / Cilt: 9 Sayı: 4

Growth-Inhibitory Effects of Traditional Chemotherapeutic Combinations (FOLFOX, FOLFIRI) on Colon Cancer Cells: An in vitro Study

Geleneksel Kemoterapötik Kombinasyonların (FOLFOX, FOLFIRI) Kolon Kanseri Hücreleri Üzerindeki Büyüme-Engelleyici Etkileri: Bir in vitro Çalışma

Aslı Yıldırım Kocaman(IĞDIR ÜNİVERSİTESİ, IĞDIR SAĞLIK HİZMETLERİ MESLEK YÜKSEKOKULU)0000-0001-5674-2095
Seçil Erden Tayhan(TOKAT GAZİOSMANPAŞA ÜNİVERSİTESİ)0000-0001-8473-5896
Pages
349–356
Publication Date
2024-12-25
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusions: The results of this preliminary study will contribute to the development of effective and targeted clinical treatment strategies for colon cancer.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: To compare the in vitro growth-inhibitory effects of the commonly used chemotherapeutic combinations folinic acid, 5-fluorouracil, and oxaliplatin (FOLFOX) and folinic acid, 5-fluorouracil, and irinotecan (FOLFIRI) on colon cancer cells (HT29 and CaCo-2 cells).
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Key Findings & Data:Materials and Methods: The viability of HT29 and CaCo-2 cells treated with different concentrations of the FOLFOX and FOLFIRI combinations was evaluated using the MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) assay.
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Conclusion & Impact:Conclusions: The results of this preliminary study will contribute to the development of effective and targeted clinical treatment strategies for colon cancer.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Antiproliferatif etki · FOLFIRI · FOLFOX

Abstract

Objective: To compare the in vitro growth-inhibitory effects of the commonly used chemotherapeutic combinations folinic acid, 5-fluorouracil, and oxaliplatin (FOLFOX) and folinic acid, 5-fluorouracil, and irinotecan (FOLFIRI) on colon cancer cells (HT29 and CaCo-2 cells). Materials and Methods: The viability of HT29 and CaCo-2 cells treated with different concentrations of the FOLFOX and FOLFIRI combinations was evaluated using the MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) assay. Results: FOLFOX and FOLFIRI combinations exhibited varying effects on the colon cancer cell lines, with HT29 cells showing sensitivity, while CaCo-2 cells demonstrated resistance to these treatments. Conclusions: The results of this preliminary study will contribute to the development of effective and targeted clinical treatment strategies for colon cancer.

Özet

Amaç: Yaygın olarak kullanılan kemoterapötik kombinasyonlar olan folinik asit, 5-florourasil ve oksaliplatin (FOLFOX) ile folinik asit, 5-florourasil ve irinotekanın (FOLFIRI) kolon kanseri hücreleri (HT29 ve CaCo-2 hücreleri) üzerindeki in vitro büyümeyi inhibe edici etkilerini karşılaştırmak. Materyal ve Metot: FOLFOX ve FOLFIRI kombinasyonlarının farklı konsantrasyonlarıyla tedavi edilen HT29 ve CaCo-2 hücrelerinin canlılığı MTT (3-(4,5-dimetiltiazolil-2)-2,5-difeniltetrazolium bromür) testi kullanılarak değerlendirildi. Bulgular: FOLFOX ve FOLFIRI kombinasyonları kolon kanseri hücre hatları üzerinde farklı etkiler gösterdi, HT29 hücreleri duyarlılık gösterirken, CaCo-2 hücreleri bu tedavilere direnç gösterdi. Sonuç: Bu ön çalışmanın sonuçları, kolon kanseri için etkili ve hedefli klinik tedavi stratejilerinin geliştirilmesine katkıda bulunacaktır.

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Cite This Article

Kocaman, A. Y., Tayhan, S. E. (2024). Growth-Inhibitory Effects of Traditional Chemotherapeutic Combinations (FOLFOX, FOLFIRI) on Colon Cancer Cells: An in vitro Study. Online Türk Sağlık Bilimleri Dergisi, Cilt: 9 Sayı: 4, 349–356. https://doi.org/10.26453/otjhs.1538781

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