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Journals / Meandros Medical And Dental Journal / 2026 / Volume: 25 Issue: 4

HIGH PREVALENCE OF TRANSFERABLE INTEGRON-ASSOCIATED DRUG RESISTANCE IN ESCHERICHIA COLI STRAINS ISOLATED FROM BLOOD CULTURES IN A UNIVERSITY HOSPITAL IN TRABZON, TURKIYE

Trabzon, Türkiyedeki Bir Üniversite Hastanesinde Kan Kültürlerinden İzole Edilen Escherichia coli Suşlarında Transfer Edilebilir İntegronla İlişkili İlaç Direncinin Yüksek Prevalansı

Ahu Reis(KARADENİZ TEKNİK ÜNİVERSİTESİ)0000-0003-3762-0812
Osman Birol Özgümüş(RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ)0000-0002-3665-6584
Erva Rakıcı(RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ)0000-0002-4464-4882
İnci Durukan(KARADENİZ TEKNİK ÜNİVERSİTESİ)0000-0002-9789-4738
Kurtuluş Buruk(KARADENİZ TEKNİK ÜNİVERSİTESİ)0000-0001-8497-5419
Gülçin Bayramoğlu(KARADENİZ TEKNİK ÜNİVERSİTESİ)0000-0002-6103-3127
Ali Osman Kılıç(Karadeniz Teknik Üniversitesi)0000-0002-5506-0866
Pages
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Publication Date
2024-12-22
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Further research is needed to understand the prevalence of E. coli strains of blood origin and the role of integrons and gene cassette arrays in the spread of resistance.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: This study aimed to determine the carriage of transferable integron-associated drug resistance in Escherichia coli (E. coli) strains isolated from blood cultures.
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Key Findings & Data:Materials and Methods: A total of 111 E. coli isolates were included in this study.
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Conclusion & Impact:Further research is needed to understand the prevalence of E. coli strains of blood origin and the role of integrons and gene cassette arrays in the spread of resistance.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Escherichia coli · Gene cassette · Integron

Abstract

Objective: This study aimed to determine the carriage of transferable integron-associated drug resistance in Escherichia coli (E. coli) strains isolated from blood cultures. Materials and Methods: A total of 111 E. coli isolates were included in this study. Antimicrobial susceptibility testing of the isolates against 17 antibiotics was performed using an automated microbiology system. Integron-specific polymerase chain reactions (PCR) were used to detect the presence of integrons. The antibiotic resistance gene cassettes in the variable regions of integrons were analyzed by DNA sequencing. Plasmid transfer assays were performed using the broth mating method. The clonal relationships among integron-carrying strains were evaluated by pulsed-field gel electrophoresis (PFGE). Results: Resistance rates to antibiotics ranged from 0.9% to 63%. Thirty-eight strains carried gene cassettes encoding dfrA7, dfr17-aadA5, dfrV, dfrA1-aadA1, and dfrA12-aadA2. Seven strains possessed class 2 integrons with gene arrays dfrA1-sat2-aadA1 and dfrA1-sat2-aadA30. Twenty-two integron-carrying isolates harbored conjugative resistance plasmids, three of which were identified as belonging to the IncN group. Two strains with class 1 integrons, isolated from different clinics, exhibited similar patterns in the PFGE analysis. Conclusion: Approximately 50% of E. coli isolates from blood cultures at our hospital were found to carry integron-associated transferable drug resistance, suggesting their potential role in the horizontal dissemination of resistance genes. Further research is needed to understand the prevalence of E. coli strains of blood origin and the role of integrons and gene cassette arrays in the spread of resistance.

Özet

Giriş: Bu çalışmada, Karadeniz Teknik Üniversitesi Hastanesi Araştırma ve Uygulama Merkezi'nde kan kültürlerinden izole edilen Escherichia coli (E. coli) suşlarının transfer edilebilir integron ilişkili ilaç direncine sahip olup olmadığının belirlenmesi amaçlanmıştır. Materyal ve Metod: İntegronların varlığı, integrona özgü polimeraz zincir reaksiyonları ile belirlendi ve amplikonlar, DNA dizilimi ile analiz edildi. Plazmid transfer deneyleri, sıvıda çiftleşme yöntemiyle gerçekleştirildi. İntegron içeren suşların klonal ilişkisi pulsed field jel elektroforezi (PFGE) ile değerlendirildi. Bulgular: Kullanılan antibiyotik gruplarına direnç oranları %0,9 ile %63 arasındaydı. Otuz sekiz (%34,2) suş, dfrA7, dfr17-aadA5, dfrV, dfrA1-aadA1 ve dfrA12-aadA2 gen kaset dizilerini taşıyan sınıf 1 integronları içeriyordu. Yedi (%6,3) suş, dfrA1-sat2-aadA1 ve dfrA1-sat2-aadA30 gen kaset dizilerine sahip sınıf 2 integronları içeriyordu. İntegron taşıyan izolatlarda 22 konjugatif direnç plazmidi barındırıldı ve bunlardan üçünün IncN grubunda olduğu belirlendi. Farklı kliniklerden izole edilen sınıf 1 integronları taşıyan dört suş, PFGE analizinde klonal olarak benzer modeller gösterdi. Sonuç: Hastanemizdeki E. coli izolatlarının yaklaşık %50'sinin integronla ilişkili aktarılabilir ilaç direnci taşıdığı ve yatay yayılımda önemli bir rol oynayabileceği sonucuna vardık. Bu hastanede ve ülke genelinde kan kökenli E. coli suşlarında integronların taşınması arasındaki ilişkinin açıklanabilmesi için daha ileri moleküler epidemiyolojik araştırmalara ihtiyaç vardır.

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Cite This Article

Reis, A., Özgümüş, O. B., Rakıcı, E., Durukan, İ., Buruk, K., Bayramoğlu, G., Kılıç, A. O., *, A. O. K. (2024). HIGH PREVALENCE OF TRANSFERABLE INTEGRON-ASSOCIATED DRUG RESISTANCE IN ESCHERICHIA COLI STRAINS ISOLATED FROM BLOOD CULTURES IN A UNIVERSITY HOSPITAL IN TRABZON, TURKIYE. Meandros Medical And Dental Journal, Volume: 25 Issue: 4. https://doi.org/10.69601/meandrosmdj.1559113

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