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Journals / Meandros Medical And Dental Journal / 2026 / Volume: 26 Issue: 2

Diagnostic Value of Circulating miRNA-16-5p and miRNA-221-3p in Thyroid Cancer

Tiroid Kanserinde Dolaşımdaki miRNA-16-5p ve miRNA-221-3p'nin Tanısal Değeri

Esin Oktay(Department of Medical Oncology, Adnan Menderes University Faculty of Medicine)0000-0002-5974-6339
Merve Bıyıklı Alemdar(Department of Medical Oncology, Adnan Menderes University Faculty of Medicine)0000-0001-6833-8217
Bilgin Demir(Department of Medical Oncology, Adnan Menderes University Faculty of Medicine)0000-0003-4380-9419
İbrahim Halil Erdoğdu(Department of Pathology, Adnan Menderes University Faculty of Medicine)0000-0002-5445-2649
Nesibe Kahraman Çetin(Department of Pathology, Adnan Menderes University Faculty of Medicine)0000-0002-4549-1670
İmran Kurt Omurlu(Department of Biostatistics, Adnan Menderes University Faculty of Medicine)0000-0003-2887-6656
Engin Güney(Department of Endocrinology, Adnan Menderes University Faculty of Medicine)0000-0001-9846-2016
Mustafa Gökhan Ünsal(General Surgery Specialist, Private Practice, Aydın, Turkey)0000-0002-6691-7511
Pages
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Publication Date
2025-06-23
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: It was observed that miRNA-221-3p and miRNA-16-5p values had diagnostic value in predicting thyroid malignancy and could be potential biomarkers.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The aim of this study is to investigate the usability of microRNA16-5P and 221-3P levels as biomarkers in determining thyroid cancers.
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Key Findings & Data:A total of 142 patients who agreed to participate in the study had 3-5 cc venous blood taken into EDTA tubes and biochemistry tubes before the operation.
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Conclusion & Impact:Conclusion: It was observed that miRNA-221-3p and miRNA-16-5p values had diagnostic value in predicting thyroid malignancy and could be potential biomarkers.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:miRNA · Biomarkers · Thyroid cancer

Abstract

Objective: The aim of this study is to investigate the usability of microRNA16-5P and 221-3P levels as biomarkers in determining thyroid cancers. Material and Methods: Patients who underwent thyroid surgery due to suspicious thyroid nodules were included in the study. A total of 142 patients who agreed to participate in the study had 3-5 cc venous blood taken into EDTA tubes and biochemistry tubes before the operation. In the postoperative period, 68 patients with malignant pathology results and 74 patients with benign pathology results were grouped as the control group. miRNA16-5p and miRNA221-3p levels were measured in serum samples taken from the patients. The levels of miRNA levels in malignant and benign patient groups were analyzed. Results: The miRNA 16-5p and miRNA221-3p levels of malignant patients were statistically significantly lower than those of benign patients (p<0.001). The analysis showed that miRNA-221-3p and miRNA-16-5p values had diagnostic value in predicting thyroid malignancy. Using a cut-off value of 21.69 for miRNA-221-3p, ROC curve analysis detected 89.7% sensitivity and 71.4% specificity (AUC=0.779, p<0.001). Using a cut-off value of 15.34 for miRNA-16-5p, ROC curve analysis detected 32.3% sensitivity and 100% specificity (AUC=0.708, p<0.001). Conclusion: It was observed that miRNA-221-3p and miRNA-16-5p values had diagnostic value in predicting thyroid malignancy and could be potential biomarkers.

Özet

Amaç: Bu çalışmanın amacı tiroid kanserlerini belirlemede mikroRNA16-5P ve 221-3P düzeylerinin biyobelirteç olarak kullanılabilirliğini araştırmaktır. Gereç ve Yöntemler: Şüpheli tiroid nodülü nedeniyle tiroid operasyonu yapılan hastalar çalışmaya alındı. Çalışmaya katılmayı kabul eden toplam 142 hastadan operasyon öncesi EDTA’lı tüpe ve biyokimya tüpüne 3-5 cc venöz kanları alındı. Ameliyat sonrası dönemde patoloji sonuçlarına göre patoloji sonucu malign olan 68 hasta ile patoloji sonucu benign olan 74 hasta kontrol grubu olarak gruplandırıldı. Hastalardan alınan serum örneklerinde miRNA-16-5p ve miRNA-221-3p düzeyleri ölçüldü. miRNA düzeylerinin malign ve benign hasta gruplarındaki düzeyleri analiz edildi. Sonuçlar: Malign hastaların miRNA-16-5p ve miRNA-221-3p düzeyleri benign hastalara göre istastistiksel olarak anlamlı derecede daha düşük saptandı (p<0.001). Yapılan analizde miRNA-221-3p ve miRNA-16-5p değerlerinin tiroid malignitesini öngörmede tanısal değeri olduğu görüldü. miRNA-221-3p için 21.69’luk bir kesme değeri kullanılarak, ROC eğrisi analizi %89.7’lik sensitivite ve %71.4’lik spesifite saptandı (AUC=0.779, p<0.001). miRNA-16-5p için 15.34’luk bir kesme değeri kullanılarak, ROC eğrisi analizi %32.3’lük sensitivite ve %100’lük spesifite saptandı (AUC=0.708, p<0.001). Sonuç: miRNA-221-3p ve miRNA-16-5p değerlerinin tiroid malignitesini öngörmede tanısal değeri olduğu, potansiyel biyobelirteç olabilecekleri görülmüştür.

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Cite This Article

Oktay, E., Alemdar, M. B., Demir, B., Erdoğdu, İ. H., Çetin, N. K., Omurlu, İ. K., Güney, E., Ünsal, M. G., *, E. O. (2025). Diagnostic Value of Circulating miRNA-16-5p and miRNA-221-3p in Thyroid Cancer. Meandros Medical And Dental Journal, Volume: 26 Issue: 2. https://doi.org/10.69601/meandrosmdj.1679264

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