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Journals / Meandros Medical And Dental Journal / 2026 / Volume: 27 Issue: 1

The Effects of Folic Acid Supplementation on Novel Biomarkers of the Endothelial Function in Rats with Experimental Periodontitis

Ebru Olgun(Periodontology Department, Faculty of Dentistry, Kirikkale University, Kirikkale, Turkey.)0000-0001-7298-8589
Oguz Kul(Veterinary Pathology Department, Faculty of Veterinary Medicine, Kirikkale University,)0000-0002-1282-650X
Nuray Ercan(BOLU ABANT İZZET BAYSAL ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ)0000-0002-8161-7269
Hasan Tarik Atmaca(Veterinary Pathology Department, Faculty of Veterinary Medicine, Balikesir University)0000-0001-8379-4114
Pages
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Publication Date
2026-03-24
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusions: The results of our study showed that periodontitis increases the expression levels of endothelial biomarkers, and FA supplementation positively impacts the endothelium dysfunction caused by periodontitis.

📌 3 Key Takeaways

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Objective & Hypothesis:Background: It is now well known that periodontitis increases the risk of future cardiovascular disease, regardless of well-known risk factors for cardiovascular disease.
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Key Findings & Data:Materials and methods: 48 rats were divided randomly into five groups: healthy control (HC), sham control (SC), lipopolysaccharide-induced periodontitis (LPS), lipopolysaccharide-induced periodontitis with low-dose FA (4 mg/kg) (LPS+4), and high-dose FA (12 mg/kg) (LPS+12) administered groups.
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Conclusion & Impact:Conclusions: The results of our study showed that periodontitis increases the expression levels of endothelial biomarkers, and FA supplementation positively impacts the endothelium dysfunction caused by periodontitis.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:folic acid · experimental periodontitis · Cardiovascular disease

Abstract

Background: It is now well known that periodontitis increases the risk of future cardiovascular disease, regardless of well-known risk factors for cardiovascular disease. The aim of this study is to investigate the effect of folic acid (FA) supplementation on endothelial damage caused by periodontitis. Materials and methods: 48 rats were divided randomly into five groups: healthy control (HC), sham control (SC), lipopolysaccharide-induced periodontitis (LPS), lipopolysaccharide-induced periodontitis with low-dose FA (4 mg/kg) (LPS+4), and high-dose FA (12 mg/kg) (LPS+12) administered groups. Rats were euthanized after 15 days of the first lipopolysaccharide injection. Circulating progenitor cells taken from each rat's blood samples were counted using flow cytometry. ADMA, CD34+, and CD133+ expression in periodontal and cardiac tissues were labeled by using immunoperoxidase method. Results: There were no differences among the groups regarding ADMA expression in periodontal tissues. However, the CD34+ and CD133+ expression values reached the highest value in the LPS group, significantly higher than in the healthy group. In the heart tissue, all biomarkers were statistically significant among groups. Flow cytometry analysis results did not differ in ADMA expression level, but CD34+ and CD133+ expression levels were statistically significant between groups. Conclusions: The results of our study showed that periodontitis increases the expression levels of endothelial biomarkers, and FA supplementation positively impacts the endothelium dysfunction caused by periodontitis.

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Cite This Article

Olgun, E., Kul, O., Ercan, N., Atmaca, H. T. (2026). The Effects of Folic Acid Supplementation on Novel Biomarkers of the Endothelial Function in Rats with Experimental Periodontitis. Meandros Medical And Dental Journal, Volume: 27 Issue: 1. https://doi.org/10.69601/meandrosmdj.1662094

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