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Journals / Meandros Medical And Dental Journal / 2026 / Volume: 27 Issue: 2

The Effects of Systemic Boric Acid Administration in Pregnant Rats with Experimentally Induced Periodontitis

Deneysel Olarak Periodontitis Oluşturulmuş Gebe Sıçanlarda Sistemik Borik Asit Uygulamasının Etkileri

Nazlı Çil(Pamukkale University)0000-0002-2164-8688
Havva Müge Balseven(Dent 20 Oral and Dental Health Center)0000-0003-2924-7771
Alper Kızıldağ(PAMUKKALE UNIVERSITY, FACULTY OF DENTISTRY, DEPARTMENT OF CLINICAL SCIENCES, DEPARTMENT OF PERIODONTOLOGY)0000-0002-1630-3140
Şahika Pınar Akyer(PAMUKKALE UNIVERSITY, SCHOOL OF MEDICINE)0000-0002-6932-3321
Gül Neşet(PAMUKKALE UNIVERSITY, SCHOOL OF MEDICINE)0000-0001-8184-5117
Ergun Mete(PAMUKKALE UNIVERSITY, SCHOOL OF MEDICINE)0000-0002-0854-2440
Canan Önder(ANKARA MEDİPOL UNIVERSITY, FACULTY OF DENTISTRY, DEPARTMENT OF CLINICAL SCIENCES (FACULTY OF DENTISTRY), DEPARTMENT OF PERIODONTOLOGY)0000-0002-8995-6361
Yaşar Enli(PAMUKKALE UNIVERSITY, SCHOOL OF MEDICINE)0000-0001-5080-3192
Gülçin Abban Mete(PAMUKKALE UNIVERSITY, SCHOOL OF MEDICINE)0000-0001-6794-3685
Pages
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Publication Date
2026-06-24
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

But the 6 mg/kg BA was found to be ineffective.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: Periodontitis causes periodontal inflammation and destruction and is known to increase the risk of occurrence in pregnant women.
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Key Findings & Data:Materials and Methods: Sixty Wistar Albino female rats were divided into six groups: A periodontally healthy, non-pregnant control group (NL,n:10); A periodontally healthy, pregnant control group (NLP,n:10); A periodontitis and pregnant group not administered BA (LP,n:10); A periodontitis, pregnant group administered 1.5 mg/kg/day BA (BA1,n:10); Periodontitis and pregnant group administered 3 mg/kg/day BA (BA3,n:10); Periodontitis and pregnant group administered 6 mg/kg/day BA (BA6,n:10).
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Conclusion & Impact:But the 6 mg/kg BA was found to be ineffective.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Periodontitis · Pregnancy · boric acid

Abstract

Aim: Periodontitis causes periodontal inflammation and destruction and is known to increase the risk of occurrence in pregnant women. Boric acid (BA) reduces inflammatory cell infiltration in periodontitis. The aim of the study is to examine the effect of BA at different doses in pregnant rats with experimentally induced periodontitis by ligature. Materials and Methods: Sixty Wistar Albino female rats were divided into six groups: A periodontally healthy, non-pregnant control group (NL,n:10); A periodontally healthy, pregnant control group (NLP,n:10); A periodontitis and pregnant group not administered BA (LP,n:10); A periodontitis, pregnant group administered 1.5 mg/kg/day BA (BA1,n:10); Periodontitis and pregnant group administered 3 mg/kg/day BA (BA3,n:10); Periodontitis and pregnant group administered 6 mg/kg/day BA (BA6,n:10). Blood and tissue samples were taken from rats. Interleukin 33 (IL-33), tumor necrosis factor α(TNF-a), interleukin-6(IL-6), interleukin-1β(IL-1β) and (The receptor activator of NF-kappaB ligand) RANKL expressions were determined by the Enzyme-linked immunosorbent assay (ELISA) and immunohistochemically. Malondialdehyde (MDA) and glutathione (GSH) measurements were performed in liver and placental tissues. Periodontium, liver and placenta tissues were evaluated histopathologically. Results: When the periodontal samples of the groups were compared, BA treatment improved histological appearance and scoring. The significant placental degeneration observed in the LP group was found to decrease in the BA3 group (p<0.05). RANKL expression was strongly positive in the LP and BA6 groups, while it weakened in the BA1 and BA3 groups. Conclusion: 1.5 mg/kg and 3 mg/kg BA were effective in treating periodontitis in pregnant rats. But the 6 mg/kg BA was found to be ineffective.

Özet

Amaç: Periodontitis, periodontal inflamasyon ve yıkıma neden olur ve hamile kadınlarda görülme riskini artırdığı bilinmektedir. Borik asit (BA), periodontitisde inflamatuar hücre infiltrasyonunu azaltır. Çalışmanın amacı, ligatür ile deneysel olarak periodontitis indüklenen hamile sıçanlarda farklı dozlarda BA'nın etkisini incelemektir. Gereç ve Yöntem: Altmış Wistar Albino dişi sıçan altı gruba ayrılmıştır: Periodontal açıdan sağlıklı, hamile olmayan kontrol grubu (NL, n:10); Periodontal açıdan sağlıklı, hamile kontrol grubu (NLP, n:10); BA uygulanmayan periodontitisli ve hamile grup (LP, n:10); Periodontitisli, hamile ve 1,5 mg/kg/gün BA verilen grup (BA1, n:10); Periodontitisli ve hamile ve 3 mg/kg/gün BA verilen grup (BA3, n:10); Periodontitisli ve hamile ve 6 mg/kg/gün BA verilen grup (BA6, n:10). Sıçanlardan kan ve doku örnekleri alınmıştır. Interlökin 33 (IL-33), tümör nekroz faktörü α (TNF-a), interlökin-6 (IL-6), interlökin-1β (IL-1β) ve (NF-kappaB ligandı reseptör aktivatörü) RANKL ekspresyonları, Enzim Bağlı İmmünosorbent Testi (ELISA) ve immünohistokimyasal olarak belirlendi. Karaciğer ve plasenta dokularında malondialdehit (MDA) ve glutatyon (GSH) ölçümleri yapıldı. Periodontiyum, karaciğer ve plasenta dokuları histopatolojik olarak değerlendirildi. Bulgular: Grupların periodontal örnekleri karşılaştırıldığında, BA tedavisi histolojik görünümü ve puanlamayı iyileştirdi. LP grubunda gözlenen önemli plasental dejenerasyonun, BA3 grubunda azaldığı bulundu (p<0,05). RANKL ekspresyonu LP ve BA6 gruplarında güçlü pozitif iken, BA1 ve BA3 gruplarında zayıfladı. Sonuç: 1,5 mg/kg ve 3 mg/kg BA, hamile sıçanlarda periodontitis tedavisinde etkili olmuştur. Ancak 6 mg/kg BA'nın etkisiz olduğu bulunmuştur.

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Cite This Article

Çil, N., Balseven, H. M., Kızıldağ, A., Akyer, Ş. P., Neşet, G., Mete, E., Önder, C., Enli, Y., Mete, G. A. (2026). The Effects of Systemic Boric Acid Administration in Pregnant Rats with Experimentally Induced Periodontitis. Meandros Medical And Dental Journal, Volume: 27 Issue: 2. https://doi.org/10.69601/meandrosmdj.1812033

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