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Journals / International Journal of Advances in Engineering and Pure Sciences / 2017 / Cilt: 29 Sayı: 4

Cell-type specific analysis: The use of fluorescence-activated cell sorting (FACS) in Arabidopsis thaliana leaves

Hücre Tipine Özgü Analiz : Arabidopsis thaliana Yapraklarında Floresans ile Aktive Edilmiş Hücrelerin Toplanması Yönteminin Kullanımı

Nihal Gören Sağlam
Pages
131–133
Publication Date
2017-12-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this review, it will be given information about FACS in transgenic Arabidopsis thaliana leaves which are labelled  with GFP.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Cell-specific resolution of gene activity is critical to understand specific developmental events.
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Key Findings & Data:However, such high resolution data have been difficult to obtain at a genomic level because specific types of cell need to be isolated.
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Conclusion & Impact:In this review, it will be given information about FACS in transgenic Arabidopsis thaliana leaves which are labelled  with GFP.
🔬Design:Review & Systematic Survey
📍Scope:Comprehensive Sample
⚙️Variables:FACS · GFP · Arabidopsis thaliana

Abstract

Cell-specific resolution of gene activity is critical to understand specific developmental events. However, such high resolution data have been difficult to obtain at a genomic level because specific types of cell need to be isolated. One of the most efficient method to isolate specific cells from neighboring cells in the same organ or tissue is FACS. The isolated cells can be used for genomic analysis. In this review, it will be given information about FACS in transgenic Arabidopsis thaliana leaves which are labelled  with GFP.

Özet

Gen aktivitesinin hücreye özgü çözümü özel gelişimsel olayların anlaşılmasında kritik bir rol oynamaktadır. Bunun yanında, genomik seviyede böyle yüksek çözünürlüklü verilerin elde edilmesi spesifik hücrelerin izole edilmesini gerektirdiği için oldukça zordur. Aynı organ ya da dokunun komşu hücrelerinden spesifik hücrelerin izole edilebilmesi için en etkili yöntem FACS (Fluorescence-activated cell sorting=Floresans tarafından aktive edilmiş hücrelerin ayrılması) yöntemidir. İzole edilen hücreler genomik analizler için kullanılabilmektedir. Bu derlemede GFP (Green flourescent protein=Yeşil floresans protein) ile işaretlenmiş transgenik Arabidopsis thaliana bitkilerinin yapraklarında FACS uygulaması hakkında bilgi verilmiş olacaktır.

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Cite This Article

Sağlam, N. G. (2017). Cell-type specific analysis: The use of fluorescence-activated cell sorting (FACS) in Arabidopsis thaliana leaves. International Journal of Advances in Engineering and Pure Sciences, Cilt: 29 Sayı: 4, 131–133. https://doi.org/10.7240/marufbd.371519

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References (7)

  1. Birnbaum K., Jung J.W., Wang J.Y., Lambert G.M., Galbraith D.W., Benfey P.N., 2003, A gene expression map of the Arabidopsis root. Science, 302:1956-1960
  2. Birnbaum K., Jung J.W., Wang J.Y., Lambert G.M., Hirst, J.A., Galbraith D.W., Benfey P.N., 2005, Cell type-specific expression profiling in plants via cell sorting of protoplasts from fluorescent repeater lines. Nat. Methods., 2:615-619
  3. Carter A.D., Bonyadi R., Gifford M.L., 2013, The use of fluorescence-activated cell sorting in studying plant development and environmental responses. Int. J. Dev. Biol., 57:545-552
  4. Gronlund J.T., Eyres A., Kumar S., Buchanan-Wollaston V., Gifford M.L., 2012, Cell specific analysis of Arabidopsis leaves using flourescence activated cell sorting, J. Vis. Exp. (68), e4214 10.3791/4214 DOI: 10.37914214 (2012)
  5. Hertberg M., Sievertzaon M., Aspeborg H., Nilsson P., Sandberg G., Lundeberg J., cDNA microarray analysis of small plant tissue samples using cDNA target amplification protocol. Plant J., 25:585-591
  6. Lee J.Y., Levesque M., Benfey P.N., 2005, High-throughput RNA isolation technologies. New tools for high-resolution gene expression profiling in plant systems. Plant Physiol., 138:585-590
  7. Wang Y. and Jiao Y., 2011, Advances in plant cell type-spesific genome-wide studies of gene expression. Front. Biol., 6:384-389