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Journals / Hacettepe Journal of Biology and Chemistry / 2026 / Volume: 53 Issue: 4

Fabrication of Antibacterial Cellulose Nanofibers via Silver Nanoparticles

Aykut Arif Topçu(Medical Laboratory Program, Vocational School of Health Services, Aksaray University, Aksaray, Türkiye)0000-0002-5234-4920
Murat İnal(Kırıkkale University, Faculty of Engineering and Natural Sciences, Department of Bioengineering)0000-0003-3384-5627
Monireh Bakhshpour-yucel(Department of Chemistry, Bursa Uludag University, Bursa, Turkey)0000-0002-5737-720X
Necdet Saglam(HACETTEPE UNIVERSITY, INSTITUTE OF SCIENCE, DEPARTMENT OF NANOTECHNOLOGY AND NANOMEDICINE, NANOTECHNOLOGY AND NANOMEDICINE (DR))0000-0002-5463-8355
Adil Denizli(HACETTEPE UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF CHEMISTRY, CHEMISTRY PR.)0000-0001-7548-5741
Pages
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Publication Date
2025-10-01
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Spectrophotometer analyses revealed the formation of AgNPs in solution, and the zeta results indicated that the size of the obtained particles was around 150 nm.

📌 3 Key Takeaways

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Objective & Hypothesis:In this work, silver nanoparticles (AgNPs) were produced on antibacterial cellulose nanofibers obtained from Acetobacter xylinum using sodium citrate through in situ formation.
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Key Findings & Data:Following the characterisation studies, the antibacterial performance of the nanofibers was investigated on gram-positive and gram-negative bacterial species.
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Conclusion & Impact:Spectrophotometer analyses revealed the formation of AgNPs in solution, and the zeta results indicated that the size of the obtained particles was around 150 nm.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:antibacterial · bacterial cellulose · Acetobacter xylinum

Abstract

In this work, silver nanoparticles (AgNPs) were produced on antibacterial cellulose nanofibers obtained from Acetobacter xylinum using sodium citrate through in situ formation. The produced nanofibers were then characterised using UV-VIS spectrophotometry, scanning electron microscopy (SEM), particle size analyses, atomic absorption spectrophotometry (AAS), and swelling studies. Following the characterisation studies, the antibacterial performance of the nanofibers was investigated on gram-positive and gram-negative bacterial species. Spectrophotometer analyses revealed the formation of AgNPs in solution, and the zeta results indicated that the size of the obtained particles was around 150 nm. SEM analyses showed that AgNPs were successfully produced on cellulose nanofibers. Swelling tests displayed that AgNP loading reduced the water-retention capacity of cellulose nanofibers by up to five times. Antibacterial efficacy tests on cellulose nanofibers containing AgNPs demonstrated their effectiveness against both gram-positive and gram-negative bacteria. The rapid formation of AgNPs on cellulose nanofibers and their high antibacterial activity demonstrate their potential for practical applications in the field of health.

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Cite This Article

Topçu, A. A., İnal, M., Bakhshpour-yucel, M., Saglam, N., Denizli, A. (2025). Fabrication of Antibacterial Cellulose Nanofibers via Silver Nanoparticles. Hacettepe Journal of Biology and Chemistry, Volume: 53 Issue: 4. https://doi.org/10.15671/hjbc.1740681

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