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Journals / Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi / 2026 / Cilt: 40 Sayı: 1

New Aptamer Pairs as a Candidate for the Rapid Detection of Food Pathogen, Salmonella enteritidis

Gıda Patojeni Salmonella enteritidis’in Hızlı Teşhisi için Aday Yeni Aptamer Çiftleri

Dilek Çam Derin(İNÖNÜ ÜNİVERSİTESİ)
Pages
1–9
Publication Date
2024-04-30
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was observed that 1st aptamer pairs are suitable for developing the LFAs for the recognition of S. enteritidis compared to the other pairs and M180 membrane was found as suitable membrane in terms of the flow rate and positive results.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Food pathogens are crucial for human and animal health.
📊
Key Findings & Data:Vomitting, diarrhea, fever and abdominal cramps are typcial symptoms occuring at 12-72 hour after the infection and can cause to death.
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Conclusion & Impact:It was observed that 1st aptamer pairs are suitable for developing the LFAs for the recognition of S. enteritidis compared to the other pairs and M180 membrane was found as suitable membrane in terms of the flow rate and positive results.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:S. enteritidis · Aptamer pairs · Lateral flow assay

Abstract

Food pathogens are crucial for human and animal health. S. enteritidis, enteric bacteria, is one of the most popular pathogen and causes salmonellosis. Vomitting, diarrhea, fever and abdominal cramps are typcial symptoms occuring at 12-72 hour after the infection and can cause to death. Food based infections in the USA and EU are primarily caused by S. enteritidis. Thus, early and rapid detection of bacteria is always highly significant. In this sense, lateral flow assays (LFAs) are notably advantageous with regards to the obtaining rapid results and practical use. LFAs are commonly prepared by antibodies and aptamers. Aptamers are single stranded DNA and RNA molecules and bind to the target molecules, selectively. Gold nanoparticles (AuNPs) are the most common labels used for LFAs. In this work, it is aimed to develop the aptamer paired strip assay (APSA) platform by using aptamer pairs specific to S. enteritidis. It was observed that 1st aptamer pairs are suitable for developing the LFAs for the recognition of S. enteritidis compared to the other pairs and M180 membrane was found as suitable membrane in terms of the flow rate and positive results.

Özet

Gıda patojenleri insan ve hayvan sağlığı için çok önemlidir. Enterik bir bakteri olan S. enteritidis en önemli patojenlerden biridir ve salmonellosise sebep olur. Kusma, ishal, ateş ve abdominal kramplar enfeksiyondan 12-72 saat sonra oluşan tipik semptomlardır ve ölüme sebep olabilir. ABD ve Avrupa’da gıda bazlı enfeksiyonlar öncelikle S. enteritidis’ten kaynaklanır. Bu nedenle bakterinin erken ve hızlı teşhisi her zaman oldukça önemlidir. Bu anlamda yanal akışlı testler (LFAs) hızlı sonuçlar alma ve pratik kullanım açısından oldukça avantajlıdır. LFA’ler yaygın olarak antikor ve aptamerler ile hazırlanır. Aptamerler tek zincirli DNA ve RNA molekülleridir ve hedef moleküle seçici şekilde bağlanır. Altın nanoparçacıklar (AuNPs) LFA’lerde en yaygın kullanılan işaretleyicilerdir. Bu çalışmada S. enteritidis’e özgün aptamer çiftleri kullanılarak aptamer çiftli strip test (APSA) platformu geliştirilmesi amaçlanmıştır. 1. aptamer çiftinin diğer çiftlerle karşılaştırıldığında S. enteritidis teşhisi için LFA geliştirilmesinde uygun olduğu gözlenmiştir ve M180 membran akış oranı ve pozitif sonuçlar açısından uygun membran olarak bulunmuştur.

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Cite This Article

Derin, D. Ç. (2024). New Aptamer Pairs as a Candidate for the Rapid Detection of Food Pathogen, Salmonella enteritidis. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi, Cilt: 40 Sayı: 1, 1–9. https://ajindex.com/articles/1266737

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