SavedAdd your journal

Journals / Etlik Veteriner Mikrobiyoloji Dergisi / 1994 / Cilt: 7 - Sayı: 5

Determination of the antibody levels of the cattle immunised against FMD by NT and ELISA

Şap Aşılı Sığırlarda Mikronötralizasyon Ve ELİSA İle Antikor Düzeylerinin Saptanması

Burhan GÜRHAN
Erdoğan ŞENEL
Gülhan DAKILIR
Hasan ÖZTÜRKMEN
Pages
99–108
Publication Date
1994-12-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In comparison of the two tesıs he sensitivity of ELISA in measuring the antibodies which positively effect the immunity have been found more than MN test.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Simple, cheep and reproducible serological procedures are required to determine foot and mouth disease (FMD) immune response of the vaccinated cattle in the field.
📊
Key Findings & Data:The antibody levels of the blood sera of the vaccinated cattle in the field hava been meaured by both microneutralisation (MN) and liquid phase sandwich enzyme linked immunosorbant assay (ELISA) techniques which were developed for that purpose, comperatively. 4978 vaccinated cattle blood sera have been received from 41 provinces in Turkey and tested.
💡
Conclusion & Impact:In comparison of the two tesıs he sensitivity of ELISA in measuring the antibodies which positively effect the immunity have been found more than MN test.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:ŞAP · Şap Aşısı · Sığırlarda Şap Aşısı

Abstract

Simple, cheep and reproducible serological procedures are required to determine foot and mouth disease (FMD) immune response of the vaccinated cattle in the field. The antibody levels of the blood sera of the vaccinated cattle in the field hava been meaured by both microneutralisation (MN) and liquid phase sandwich enzyme linked immunosorbant assay (ELISA) techniques which were developed for that purpose, comperatively. 4978 vaccinated cattle blood sera have been received from 41 provinces in Turkey and tested. As a result, the true prevalance of the immunity among the vaccinated cattle was estimated as 81,65% against A22 and 76,15% against o, types. In comparison of the two tesıs he sensitivity of ELISA in measuring the antibodies which positively effect the immunity have been found more than MN test.

Özet

Şap hastalığına karşı sahadaki aşılı sığırlarda oluşan immun yanıtı tesbit etmek için uygulaması kolay, ucuz, aynı zamanda çok fazla materyal ile çalışmaya elverişli serolajik teknikiere ihtiyaç vardır. Bu amaçla geliştirilmiş olan mikronötralizasyon ve liquid phase sandwich ELISA (enzyme- linked immunosorbant assay) ile karşılaştırmalı olarak sahada aşılı sığırlardan alınan kan serumlarının antikor düzeyleri ölçüldü. 41 ilde şap hastalığına karşı aşılı sığırlardan alınan toplam 4978 adet sığır kan serumu numunesi test edildi. Veriler istatistik yöntemler ile değerlendirildi. Sonuçta bağışıklığın gerçek prevalansı A22 tipi için% 81,65, 0 1 tipi için% 76,15 olarak tesbit edilirken iki metot arasında yapılan karşılaştırmada ELISA'nın antikor pozitif serumları tanımlamada seronötralizasyon teste oranla daha hassas olduğu görüldü.

🔖

Cite This Article

GÜRHAN, B., ŞENEL, E., DAKILIR, G., ÖZTÜRKMEN, H. (1994). Determination of the antibody levels of the cattle immunised against FMD by NT and ELISA. Etlik Veteriner Mikrobiyoloji Dergisi, Cilt: 7 - Sayı: 5, 99–108. https://ajindex.com/articles/125656

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (16)

  1. ABU-ELZEIN, E.M.E. and COUWTHER, J.R.:The specific detection of FMDV whole antifen by ELISA. J. Hyg. Camb., 83, 127-134. 1979.
  2. ARIAS, C.A.L., HANSON, R.P. and GERARDINO, A.G.: Antibody response of tropical tests. lnt. Symp. FMD, Lyon, 1976. Dev. Stand., 35, 345-356 (S. Karger, Basel197). BAILEY, N.T.J.: Statistical Mathods in Biology, See. Ed., Edward Arnoldof Hadder and Stoughton, London, 1981.
  3. DUBOURGET, P., DTERAZ, N., GARGON, P. and LOMBARD, M.: Serological and biochemical properties of FMD strains recently isolated in Near and Meadle-East. 1. Int. FMD. Symp., June, 1989.
  4. Ongun Kard. Mat., Ankara, 1990.
  5. DOEL, T.R.: The immuna response of cattle to FMD antigens. Pitman-Moore FMD Bulletin, 29, 1, 1-5. 1991.
  6. FEDIDA, M., DANNACHER, G., COUDERT, M., PEILLON, M. and LUCAM, F.: Evelution et duree oe l'immunite antiaphteuse post-vaccinale chez les bovins pluri-vaccines. Bull. Off. Int. Epizoot. 77, 1005-027. 1972.
  7. HAMBLIN, C., BARNETI, I.T.R., CROWTER, J.R.: Anew ELI SA for detection of antibodies against FMDV. 1. Development and method of ELISA. J. Imm. Me.th. 93, 115-121.1986.
  8. HAMBLIN, C., KITCHING, R.P., OONALOSON, A.l., CROWTER, J.R. and BARNETI, T.R.: ELISA for the detection of antibodies against FMDV. Epidem. Inf., 99, 733-744.1987.
  9. LOMBARO, M.: Utulisation de serums de bovins vaccinesdans l'evaluation des souches virales aphteuses du terrain. Sess. Res. Gr. Stand. Tech. Comm. Eur: Comm. Cent. FMO, Lindholm, Oenmark, FAO, Rome, 1979.
  10. LOMBARD, M. und PETERMAN, H.G.: Korrelation zwischen der ELISA-Test und der Serumneutralisation zur Titrierung MKS-Virus-neutralisierender antikörper im Serum von Kontrollrindern. Tierarzt. Umschau, 37. 354-356. 1982
  11. MACKOWIAK, C., LANG C., FONTAINE, J., CAMANO, R., PETERMAN, H.G.: Relation between neutralising antibody titre and protection in animals immunized against FMD. Ann. lnst. Pasteur, 103, 252-257. 1962.
  12. MCCULLOGH, K.C., ACKERMANN, M., MÜLLER, H.K., BRUCKNER, L. and KIHM, U.: Potaney testing of FMD vaccines by challenge and antibody measurement. 1. Int. FMD Symp., June, 1989.
  13. Ongun Kard. Mat., Ankara. 1989.
  14. OUCHTERLONY, O.: Oiffusion in gels methodsfor immunological analyses. Prog. n Allergy, 5, 1-78. ed. by p. Kallas, Karger-Basel, 1958
  15. PANINA, G. and DeSIMONE, F.: Risposta anticorpale dei bovini adiverse dosidi vaccino antiaphtoso. Confronto ıra siero-neutralizzazione ed inibizione della fissazione del complenıento. Atti Della Societa haliana Della Scienze Veterinaire, 25, 518-520. 1971.
  16. PAY, T.W.F.