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Journals / European Oral Research / 2026 / Volume: 50 Issue: 2

OSTEOCALCIN AND OSTEONECTIN EXPRESSION AFTER DOUBLE-APPLICATION OF PLATELET-RICH PLASMA IN RABBITS

Burcu Çakılcı Özdemir
Bülent Kurtiş
Gülay Tüter
Burcu Şengüven
Benay Yıldırım
Gönen Özcan
Pages
—
Publication Date
2016-04-01
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: The present findings suggest that DAPRP did not have a significant effect on the healing of non-critical size rabbit cranial bone defects.

📌 3 Key Takeaways

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Objective & Hypothesis:Purpose: Platelet-rich plasma (PRP) is a novel method for transferring autogenous growth factors to the wound area.
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Key Findings & Data:Materials and Methods: Twenty-eight rabbits, each with two surgically prepared calvarial bone defects, were included in this study and divided into six groups: The defects (N=56) were treated with either a single-application of PRP (SA-PRP) (n=10), a combination of SA-PRP and betatricalciumphosphate (SA-PRP+β-TCP) (n=10), only DAPRP (n=8), both DA-PRP and beta-tricalciumphosphate (DA-PRP+β-TCP) (n=8), only beta-tricalciumphosphate (β-TCP) (n=10), or controls (n=10).
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Conclusion & Impact:Conclusion: The present findings suggest that DAPRP did not have a significant effect on the healing of non-critical size rabbit cranial bone defects.
🔬Design:Article
📍Scope:Comprehensive Sample
⚙️Variables:Platelet-Rich Plasma · osteonectin · osteocalcin

Abstract

Purpose: Platelet-rich plasma (PRP) is a novel method for transferring autogenous growth factors to the wound area. The aim of this study was to evaluate the efficacy of double-application of PRP (DA-PRP) on bone healing in rabbit cranial defects by examining osteonectin (ON) and osteocalcin (OC) expression. Materials and Methods: Twenty-eight rabbits, each with two surgically prepared calvarial bone defects, were included in this study and divided into six groups: The defects (N=56) were treated with either a single-application of PRP (SA-PRP) (n=10), a combination of SA-PRP and betatricalciumphosphate (SA-PRP+β-TCP) (n=10), only DAPRP (n=8), both DA-PRP and beta-tricalciumphosphate (DA-PRP+β-TCP) (n=8), only beta-tricalciumphosphate (β-TCP) (n=10), or controls (n=10). The animals were sacrificed at 30th day postoperatively and samples were immunohistochemically examined for ON and OC expressions. Results: It was determined that DA-PRP did not significantly improve the ON and OC percentages achieved by SA-PRP or the controls. The three groups treated with β-TCP showed a higher percentage of ON than those treated without β-TCP (p<0.05). The β-TCP treated groups and SA-PRP group demonstrated higher OC percentage than DA-PRP and control groups (p<0.05). Conclusion: The present findings suggest that DAPRP did not have a significant effect on the healing of non-critical size rabbit cranial bone defects.

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Cite This Article

Özdemir, B. Ç., Kurtiş, B., Tüter, G., Şengüven, B., Yıldırım, B., Özcan, G. (2016). OSTEOCALCIN AND OSTEONECTIN EXPRESSION AFTER DOUBLE-APPLICATION OF PLATELET-RICH PLASMA IN RABBITS. European Oral Research, Volume: 50 Issue: 2. https://doi.org/10.17096/jiufd.40536

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References (37)

  1. Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85(6):638-646.
  2. Tsay RC, Vo J, Burke A, Eisig SB, Lu HH, Landesberg R. Differential growth factor retention by platelet-rich plasma composites. J Oral Maxillofac Surg 2005;63(4):521-528.
  3. Kovacs K, Velich N, Huszar T, Szabo G, Semjen G, Reiczigel J, Suba Z. Comparative study of beta-tricalcium phosphate mixed with platelet-rich plasma versus beta-tricalcium phosphate, a bone substitute material in dentistry. Acta Vet Hung 2003;51(4):475-484.
  4. Monov G, Fuerst G, Tepper G, Watzak G, Zechner W, Watzek G. The effect of platelet-rich plasma upon implant stability measured by resonance frequency analysis in the lower anterior mandibles. Clin Oral Implants Res 2005;16(4):461-465.
  5. Lekovic V, Camargo PM, Weinlaender M, Vasilic N, Kenney EB. Comparison of platelet-rich plasma, bovine porous bone mineral, and guided tissue regeneration versus platelet-rich plasma and bovine porous bone mineral in the treatment of intrabony defects: A reentry study. J Periodontol 2002;73(2):198-205.
  6. Anitua E. Plasma rich in growth factors: Preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants 1999;14(4):529-535.
  7. Yazawa M, Ogata H, Kimura A, Nakajima T, Mori T, Watanabe N. Basic studies on the bone formation ability by platelet rich plasma in rabbits. J Craniofac Surg 2004;15(3):439-446.
  8. Kovacs K, Velich N, Huszar T, Fenyves B, Suba Z, Szabo G. Histomorphometric and densitometric evaluation of the effects of platelet-rich plasma on the remodeling of beta-tricalcium phosphate in beagle dogs. J Craniofac Surg 2005;16(1):150-154.
  9. Raghoebar GM, Schortinghuis J, Liem RS, Ruben JL, van der Wal JE, Vissink A. Does platelet-rich plasma promote remodeling of autologous bone grafts used for augmentation of the maxillary sinus floor? Clin Oral Implants Res 2005;16(3):349-356.
  10. Thorwarth M, Rupprecht S, Falk S, Felszeghy E, Wiltfang J, Schlegel KA. Expression of bone matrix proteins during de novo bone formation using a bovine collagen and platelet-rich plasma (prp)-an immunohistochemical analysis. Biomaterials 2005;26(15):2575-2584.
  11. Crovetti G, Martinelli G, Issi M, Barone M, Guizzardi M, Campanati B, Moroni M, Carabelli A. Platelet gel for healing cutaneous chronic wounds. Transfus Apher Sci 2004;30(2):145-151.
  12. Driver VR, Hanft J, Fylling CP, Beriou JM, Autologel Diabetic Foot Ulcer Study G. A prospective, randomized, controlled trial of autologous platelet-rich plasma gel for the treatment of diabetic foot ulcers. Ostomy Wound Manage 2006;52(6):68-70, 72, 74 passim.
  13. Redaelli A, Romano D, Marciano A. Face and neck revitalization with platelet-rich plasma (prp): Clinical outcome in a series of 23 consecutively treated patients. J Drugs Dermatol 9(5):466-472.
  14. Kon E, Buda R, Filardo G, Di Martino A, Timoncini A, Cenacchi A, Fornasari PM, Giannini S, Marcacci M. Platelet-rich plasma: Intra-articular knee injections produced favorable results on degenerative cartilage lesions. Knee Surg Sports Traumatol Arthrosc 18(4):472-479.
  15. Ozdemir B, Kurtis B, Tuter G, Senguven B, Tokman B, Pinar-Ozdemir S, Demirel I, Ozcan G. Double-application of platelet-rich plasma on bone healing in rabbits. Med Oral Patol Oral Cir Bucal 17(1):e171-177.
  16. Aghaloo TL, Moy PK, Freymiller EG. Investigation of platelet-rich plasma in rabbit cranial defects: A pilot study. J Oral Maxillofac Surg 2002;60(10):1176-1181.
  17. Merten HA, Wiltfang J, Grohmann U, Hoenig JF. Intraindividual comparative animal study of alpha- and beta-tricalcium phosphate degradation in conjunction with simultaneous insertion of dental implants. J Craniofac Surg 2001;12(1):59-68.
  18. Christenson RH. Biochemical markers of bone metabolism: An overview. Clin Biochem 1997;30(8):573-593.
  19. Heinegard D, Oldberg A. Structure and biology of cartilage and bone matrix noncollagenous macromolecules. FASEB J 1989;3(9):2042-2051.
  20. Termine JD, Kleinman HK, Whitson SW, Conn KM, McGarvey ML, Martin GR. Osteonectin, a bone-specific protein linking mineral to collagen. Cell 1981;26(1 Pt 1):99-105.
  21. Swaminathan R. Biochemical markers of bone turnover. Clin Chim Acta 2001;313(1-2):95-105.
  22. Lieberman JR, Daluiski A, Einhorn TA. The role of growth factors in the repair of bone. Biology and clinical applications. J Bone Joint Surg Am 2002;84-A(6):1032-1044.
  23. Fisher LW, Termine JD. Noncollagenous proteins influencing the local mechanisms of calcification. Clin Orthop Relat Res 1985, 200):362-385.
  24. Otsuka K, Yao KL, Wasi S, Tung PS, Aubin JE, Sodek J, Termine JD. Biosynthesis of osteonectin by fetal porcine calvarial cells in vitro. J Biol Chem 1984;259(15):9805-9812.
  25. Kroese-Deutman HC, Wolke JG, Spauwen PH, Jansen JA. Closing capacity of cranial bone defects using porous calcium phosphate cement implants in a rabbit animal model. J Biomed Mater Res A 2006;79(3):503-511.
  26. Zhu JX, Sasano Y, Takahashi I, Mizoguchi I, Kagayama M. Temporal and spatial gene expression of major bone extracellular matrix molecules during embryonic mandibular osteogenesis in rats. Histochem J 2001;33(1):25-35.
  27. Clarke B. Normal bone anatomy and physiology. Clin J Am Soc Nephrol 2008;3 Suppl 3(S131-139.
  28. Cowles EA, DeRome ME, Pastizzo G, Brailey LL, Gronowicz GA. Mineralization and the expression of matrix proteins during in vivo bone development. Calcif Tissue Int 1998;62(1):74-82.
  29. Wiesmann HP, Meyer U, Plate U, Hohling HJ. Aspects of collagen mineralization in hard tissue formation. Int Rev Cytol 2005;242:121-156.
  30. Boskey AL, Gadaleta S, Gundberg C, Doty SB, Ducy P, Karsenty G. Fourier transform infrared microspectroscopic analysis of bones of osteocalcin-deficient mice provides insight into the function of osteocalcin. Bone 1998;23(3):187-196.
  31. Hunter GK, Hauschka PV, Poole AR, Rosenberg LC, Goldberg HA. Nucleation and inhibition of hydroxyapatite formation by mineralized tissue proteins. Biochem J 1996;317(1):59-64.
  32. Owen TA, Aronow M, Shalhoub V, Barone LM, Wilming L, Tassinari MS, Kennedy MB, Pockwinse S, Lian JB, Stein GS. Progressive development of the rat osteoblast phenotype in vitro: Reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 1990;143(3):420-430.
  33. Velich N, Nemeth Z, Hrabak K, Suba Z, Szabo G. Repair of bony defect with combination biomaterials. J Craniofac Surg 2004;15(1):11-15.
  34. Wiltfang J, Kloss FR, Kessler P, Nkenke E, Schultze-Mosgau S, Zimmermann R, Schlegel KA. Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects. An animal experiment. Clin Oral Implants Res 2004;15(2):187-193.
  35. Harnack L, Boedeker RH, Kurtulus I, Boehm S, Gonzales J, Meyle J. Use of platelet-rich plasma in periodontal surgery--a prospective randomised double blind clinical trial. Clin Oral Investig 2009;13(2):179-187.
  36. Ozdemir B, Okte E. Treatment of intrabony defects with beta-tricalciumphosphate alone and in combination with platelet-rich plasma. J Biomed Mater Res B Appl Biomater 2012;100(4):976-983.
  37. Kondo N, Ogose A, Tokunaga K, Ito T, Arai K, Kudo N, Inoue H, Irie H, Endo N. Bone formation and resorption of highly purified beta-tricalcium phosphate in the rat femoral condyle. Biomaterials 2005;26(28):5600-5608.