Journals / Turkish Journal of Veterinary and Animal Sciences / 2005 / Cilt: 29 - Sayı: 2

Plasmids and Protein Patterns of Escherichia coli Isolated from Bovine Mastitis in Konya, Turkey

Pages
475–480
Publication Date
2005-02-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Of these two methods, SDS-PAGE typing proved to be more discriminate for typing the isolates.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, a total of 30 Escherichia coli isolates obtained from milk samples of dairy cows suffering from subclinical mastitis in Konya, Turkey were typed according to plasmids and protein patterns.
📊
Key Findings & Data:Agarose gel electrophoresis and sodium doedecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) methods were used to identify plasmids and whole-cell protein profiles.
💡
Conclusion & Impact:Of these two methods, SDS-PAGE typing proved to be more discriminate for typing the isolates.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:SDS-PAGE · plasmid · mastitis

Abstract

In this study, a total of 30 Escherichia coli isolates obtained from milk samples of dairy cows suffering from subclinical mastitis in Konya, Turkey were typed according to plasmids and protein patterns. Agarose gel electrophoresis and sodium doedecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) methods were used to identify plasmids and whole-cell protein profiles. Of these two methods, SDS-PAGE typing proved to be more discriminate for typing the isolates.

Özet

In this study, a total of 30 Escherichia coli isolates obtained from milk samples of dairy cows suffering from subclinical mastitis in Konya, Turkey were typed according to plasmids and protein patterns. Agarose gel electrophoresis and sodium doedecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) methods were used to identify plasmids and whole-cell protein profiles. Of these two methods, SDS-PAGE typing proved to be more discriminate for typing the isolates.

🔖

Cite This Article

UÇAN, U. S. (2005). Plasmids and Protein Patterns of Escherichia coli Isolated from Bovine Mastitis in Konya, Turkey. Turkish Journal of Veterinary and Animal Sciences, Cilt: 29 - Sayı: 2, 475–480. https://ajindex.com/articles/122379

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Schukken, Y.H., Kremer, W.D.J., Lohuis, J.A.C.: E. coli mastitis. bij het rund. Tijdschr. Diergenskd., 1989; 114: 829-838.
  2. Döpfer, D., Barkem, H.W., Lam, T.J.G.M., Schukken, Y.H., Gaastra, W.: Recurrent clinical mastitis caused by E. coli in dairy cows. Dairy Cows. J. Dairy Sci., 1999; 82: 80-85.
  3. Tyler, J.W., Spears, H., Erskine, R.J., Martin, J., Nelson, R.: Antigenic homology among bacteria isolated from clinical Gram- negative mastitis. Internat. Symp. on Bovine Mastitis, Indianapolis. 1990; 196-198.
  4. Hadimli, H.H., Erganifl, O.: Mastitis ve Bağıflıklık. Mastitis Sempozyumu, Akdeniz Üniversitesi, Burdur Veteriner Fakültesi. Burdur. 2001; 27-28.
  5. Fantasia, M., Ricci, N., Manuppella, A., Martini, A., Filetici, E., Laurelli, T.: Phage type and DNA plasmid of Salmonella typhimurium isolates in the area of Isernia, Italy. Epidemiol. Infect., 1990; 105: 317-323.
  6. McClure, P.J.: Microbiological hazard identification in the meat industry. HACCP in the meat industry. CRC Press Woodhead Publishing Limited, Cambridge. 2000; 157-176. 7. Parisi, J.T., Hecht, D.W.: Plasmid profiles in epidemiologic studies of infections by S. epidermidis. J. Infect. Dis., 1980; 141: 637- 643. 8. Shi, Z.H., Liu, P.Y.F., Lau, Y.J., Hu, B.S., Lin, Y.H.: Comparison of polymerase chain reaction and pulsed-field gel electrophoresis for the epidemiological typing of Campylobacter jejuni. Diag. Microbiol. Infect. Dis., 1996; 26: 103-108.
  7. Tanner, A.C.R., Listgarten, M.A., Ebersole, J.L.: Bacteroides forsythus sp. nov., a slow-growing, fusiform Bacteroides sp. from the human oral cavity. Int. J. Syst. Bacteriol., 1986; 36: 213- 221. Walia, T.M., Williamson, T., Kaiser A., Tewari, R.: Usefulness of protein patterns, serotyping and plasmid DNA profiles in the epidemiologic finger printing of Pseudomonas aeruginosa. Eur. J. Clin. Microbiol. Infect. Dis., 1988; 7: 248-255.
  8. Dorn, C., Silapanuntakul, R., Angrick, E., Shipman, L.: Plasmid analysis and epidemiology of Salmonella enteritidis infection in three commercial layer flocks. Avian Dis., 1992; 38: 844-851.
  9. Neuwirth, C., Siebor, E., Lopez, J., Pechinot, A., Kazmierczak, A.: Outbreak of TEM-24-producing Enterobacter aerogenes in an intensive care unit and dissemination of the extended-spectrum beta-lactamase to other members of the family enterobacteriaceae. J. Clin. Microbiol., 1996; 34: 76-79.
  10. Wachsmuth, I., Kiehlbauch, J., Bopp, C., Cameron, D., Strockbine, N., Wells, J.A.B.P.: The use of plasmid profiles and nucleic acid probes in epidemiologic