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Journals / Veteriner Bilimleri Dergisi . Eurasian Journal of Veterinary Sciences / 2005 / Cilt: 21 - Sayı: 1

Determination by RAPD-PCR of relationship degrees of S. aureus and S. intermedius strains isolated from bovline mastitis

Mastitisli ineklerden izole edilen S. Aureus ve S. Intermedius suşlarının RAPD-PCR ile akrabalık derecelerinin belirlenmesi

Emine ARSLAN
Leyla AÇIK
UÇKUN SAİT UÇAN
Pages
65–69
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Dendrogram obtained by RAPD results showed that ali the isolates clustered in to four-main groups at a genetic similarity of less than 30% when analyzed with 10 random primers.

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Objective & Hypothesis:The aim of this study was to determine of relationship degree with RAPD-PCR (Random Amplified Polymorphic DNA Polymerase Chain Reaction) method of 23 of S.aureus and 7 of S.intermedius strains isolated from bovine mastitis.
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Key Findings & Data:Dendrogram obtained by RAPD results showed that ali the isolates clustered in to four-main groups at a genetic similarity of less than 30% when analyzed with 10 random primers.
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Conclusion & Impact:This study shows that RAPD-PCR assayed with random primers can be successfully applied to assess the genetic relationship of S. aureus and S. intermedius strains isolated from bovine mastitis.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:rastgele çoğaltılmış polimorfik DNA · genetik uzaklık · Staphylococcus intermedius

Abstract

The aim of this study was to determine of relationship degree with RAPD-PCR (Random Amplified Polymorphic DNA Polymerase Chain Reaction) method of 23 of S.aureus and 7 of S.intermedius strains isolated from bovine mastitis. Dendrogram obtained by RAPD results showed that ali the isolates clustered in to four-main groups at a genetic similarity of less than 30% when analyzed with 10 random primers. While the highest relationship degree was 91.30%, the lowest relationship degree was found as 0.44%. This study shows that RAPD-PCR assayed with random primers can be successfully applied to assess the genetic relationship of S. aureus and S. intermedius strains isolated from bovine mastitis.

Özet

Bu çalışmanın amacı, mastitisli ineklerden izole edilen 23 S. aureus ve 7 S. intermedius suşlarının RAPD-PCR (Rast-gele Çoğaltılmış Polimorfik DNA Polimeraz Zincir Reaksiyonu) yöntemi ile akrabalık derecelerinin belirlenmesidir. RAPD-PCR sonuçları ile elde edilen dendrogram, 10 rastgele primer ile analiz edildiğinde bütün izolatlar % 30'tan daha az genetik benzerlikte 4 ana küme oluşturduğunu göstermiştir. En yüksek akrabalık derecesi %91.30 iken en düşük akrabalık derecesi %0.44 olarak bulunmuştur. Bu çalışma, ratgele primerler ile yapılmış olan RAPD-PCR'ın mastitisli ineklerden izole edilmiş S. aureus ve S. intermedius suşlarının genetik akrabalığını belirlemek için başarılı bir şekilde uygulanabileceğini göstermiştir.

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Cite This Article

ARSLAN, E., AÇIK, L., UÇAN, U. S. (2005). Determination by RAPD-PCR of relationship degrees of S. aureus and S. intermedius strains isolated from bovline mastitis. Veteriner Bilimleri Dergisi . Eurasian Journal of Veterinary Sciences, Cilt: 21 - Sayı: 1, 65–69. https://ajindex.com/articles/333439

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