SavedAdd your journal

Journals / Turkish Journal of Veterinary and Animal Sciences / 2005 / Cilt: 29 - Sayı: 2

Isolation of Arcanobacterium (Actinomyces) pyogenes from Abscessed Cattle Kidney and Identification by PCR

Hasan Basri ERTAŞ
Ayşe KILIÇ
Gökben ÖZBEY
Adile MUZ
Pages
455–459
Publication Date
2005-02-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was concluded that PCR employed in this study may be applied for identification of A. pyogenes.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, the presence of Arcanobacterium spp., which causes a variety of purulent infections involving the skin, joints and visceral organs, was investigated in abscessed kidney samples of cattle.
📊
Key Findings & Data:A total of 500 cattle were examined at postmortem and 100 samples with abscess were collected.
💡
Conclusion & Impact:It was concluded that PCR employed in this study may be applied for identification of A. pyogenes.
🔬Design:Research Article
📍Scope:100 samples
⚙️Variables:Arcanobacterium pyogenes · PCR · cattle

Abstract

In this study, the presence of Arcanobacterium spp., which causes a variety of purulent infections involving the skin, joints and visceral organs, was investigated in abscessed kidney samples of cattle. A total of 500 cattle were examined at postmortem and 100 samples with abscess were collected. Arcanobacterium pyogenes was isolated in 40 (40%) of the samples examined. DNA extracted from the isolates were amplified by Polymerase Chain Reaction (PCR) using specific primers derived from plo gene of A. pyogenes and all the isolates were determined to be positive by PCR. It was concluded that PCR employed in this study may be applied for identification of A. pyogenes.

Özet

In this study, the presence of Arcanobacterium spp., which causes a variety of purulent infections involving the skin, joints and visceral organs, was investigated in abscessed kidney samples of cattle. A total of 500 cattle were examined at postmortem and 100 samples with abscess were collected. Arcanobacterium pyogenes was isolated in 40 (40%) of the samples examined. DNA extracted from the isolates were amplified by Polymerase Chain Reaction (PCR) using specific primers derived from plo gene of A. pyogenes and all the isolates were determined to be positive by PCR. It was concluded that PCR employed in this study may be applied for identification of A. pyogenes.

🔖

Cite This Article

ERTAŞ, H. B., KILIÇ, A., ÖZBEY, G., MUZ, A. (2005). Isolation of Arcanobacterium (Actinomyces) pyogenes from Abscessed Cattle Kidney and Identification by PCR. Turkish Journal of Veterinary and Animal Sciences, Cilt: 29 - Sayı: 2, 455–459. https://ajindex.com/articles/122367

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Ramos, C.P., Foster, G., Collins, M.D.: Phylogenetic analysis of the genus Actinomyces based on 16S rRNA gene sequences: description of Arcanobacterium bernardiae comb. nov., and Arcanobacterium pyogenes comb. nov. Int. J. Syst. Bacteriol., 1997; 47: 46-53.
  2. Lechtenberg, K.F., Nagaraja, T.G., Leipold, H.W., Chengappa, M.M.: Bacteriologic and histologic studies of hepatic abscesses in cattle. Am. J. Vet. Res., 1988; 49: 58-62. 6. Timoney, J.F., Gillespie, J.H., Scott, F.W., Barlough, J.E.: Hagan and Bruner’s Microbiology and Infectious Diseases of Domestic Animals. Cornell University Press,
  3. Ithaca, NY, 1988.
  4. Barbour, E.K., Brinton, M.K., Caputa, A., Johnson, J.B., Poss, P.E.: Characteristics of Actinomyces pyogenes involved in lameness of male turkeys in north-central United States. Avian Dis., 1991; 35: 192-196.
  5. Brinton, M.K., Schellberg, L.C., Johnson, J.B., Frank, R.K., Halvorson, D.A., Newman, J.A.: Description of osteomyelitis lesions associated with Actinomyces pyogenes infection in the proximal tibia of adult male turkeys. Avian Dis., 1993; 37: 259- 262. Sokkar, S.M., Mohamed, M.A., Atawia, M.: Experimental induction of renal lesions in chickens. Berl. Munch. Tierarztl. Wschr., 1998; 111: 161-163.
  6. Jootar, P., Gherunpong, V., Saitanu, K.: Corynebacterium pyogenes endocarditis. Report of a case with necropsy and review of the literature. J. Med. Assoc. Thailand, 1978; 61: 596-601.
  7. Kotrajaras, R., Tagami, H.:Corynebacterium pyogenes. It’s pathogenic mechanism in leg ulcers in Thailand. Int. J. Dermatol., 1987; 26: 45-50
  8. Hillerton, J.E., Bramley, A.J.: Infection following challenge of the lactating and dry udder of dairy cows with Actinomyces pyogenes and Peptostreptococcus indolicus. Br. Vet. J., 1989; 145: 148- 159. Semambo, D.K.N., Ayliffe, T.R., Boyd, J.S., Taylor, D.J.: Early abortion in cattle induced by experimental intrauterine infection with pure cultures of Actinomyces pyogenes. Vet. Rec., 1991; 129: 12-16.
  9. Ruder, C.A., Sasser, R.G., Williams, R.J., Ely, J.K., Bull, R.C., Butler, J.E.: Uterine infections in the postpartum cow. II. Possible synergistic effect of Fusobacterium necrophorum Corynebacterium pyogenes. Theriogenology, 1981; 15: 573-580 and Palmer, M.V., Whipple, D.L.: Arcanobacterium pyogenes as a cause of fatal pleuropneumonia after capture and transport of white-tailed deer Odocoileus viginianus. J. Vet. Diagn. Invest., 1999; 11: 468-471
  10. Foreyt, W.J., Jessup, D.A.: Fatal pneumonia of bighorn sheep following association with domestic