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Journals / Turkish Journal of Veterinary and Animal Sciences / 2008 / Cilt: 32 - Sayı: 5

Surfactant Proteins A and D in the Seminal Plasma of Stallions

Ayhan ATA
Orhan KANKAVİ
M. Akif ÇİFTÇİOĞLU
Pages
353–358
Publication Date
2008-05-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The similarity between biochemical properties (relative molecular masses) of SP-A and SP-D in accessory sex glands and seminal plasma imply that SP-A and SP-D proteins are also present in the seminal plasma and accessory sex glands of stallions.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:We have previously shown that surfactant protein (SP)-A and SP-D are present in the stallion´s genital system.
📊
Key Findings & Data:On one-dimensional SDS-PAGE electrophoresis and western blots, bands for SP-A (34 kDa) and SP-D (43 kDa) in seminal plasma and accessory sex gland tissue extracts were detected.
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Conclusion & Impact:The similarity between biochemical properties (relative molecular masses) of SP-A and SP-D in accessory sex glands and seminal plasma imply that SP-A and SP-D proteins are also present in the seminal plasma and accessory sex glands of stallions.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Surfactant protein A (SP-A) · surfactant protein D (SP-D) · western blotting

Abstract

We have previously shown that surfactant protein (SP)-A and SP-D are present in the stallion´s genital system. The aim of the present study was to evaluate the expression and presence of SP-A and SP-D in the seminal plasma and accessory sex glands of stallions. We characterized further the SP-A and SP-D in the seminal plasma of stallions by western blotting and immunohistochemically in accessory sex glands. On one-dimensional SDS-PAGE electrophoresis and western blots, bands for SP-A (34 kDa) and SP-D (43 kDa) in seminal plasma and accessory sex gland tissue extracts were detected. The similarity between biochemical properties (relative molecular masses) of SP-A and SP-D in accessory sex glands and seminal plasma imply that SP-A and SP-D proteins are also present in the seminal plasma and accessory sex glands of stallions.

Özet

We have previously shown that surfactant protein (SP)-A and SP-D are present in the stallion´s genital system. The aim of the present study was to evaluate the expression and presence of SP-A and SP-D in the seminal plasma and accessory sex glands of stallions. We characterized further the SP-A and SP-D in the seminal plasma of stallions by western blotting and immunohistochemically in accessory sex glands. On one-dimensional SDS-PAGE electrophoresis and western blots, bands for SP-A (34 kDa) and SP-D (43 kDa) in seminal plasma and accessory sex gland tissue extracts were detected. The similarity between biochemical properties (relative molecular masses) of SP-A and SP-D in accessory sex glands and seminal plasma imply that SP-A and SP-D proteins are also present in the seminal plasma and accessory sex glands of stallions.

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Cite This Article

ATA, A., KANKAVİ, O., ÇİFTÇİOĞLU, M. A. (2008). Surfactant Proteins A and D in the Seminal Plasma of Stallions. Turkish Journal of Veterinary and Animal Sciences, Cilt: 32 - Sayı: 5, 353–358. https://ajindex.com/articles/122306

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References (15)

  1. Kankavi, O., Ata, A., Ciftcioglu, M.A.: Surfactant protein A and D in the reproductive tract of stallion. Theriogenology, 2006; 66: 1057-1064.
  2. Wu, H., Kuzmenko, A., Wan, S., Schaffer, L., Weiss, A., Fisher, J.H., Kim, K.S., McCormack, F.X.: Surfactant proteins A and D inhibit the growth of Gram negative bacteria by increasing membrane permeability. J. Clin. Invest., 2003; 111: 1589-1602.
  3. Giannoni, E., Sawa, T., Allen, L., Wiener-Kronish, J., Hawgood, S.: Surfactant proteins A and D enhance pulmonary clearance of Pseudomonas aeruginosa. Am. J. Respir. Cell Mol. Biol., 2006; 34: 704-710.
  4. Madan, T., Kishore, U., Singh, M., Strong, P., Hussain, E.M., Reid, K.B.M., Sarma, P.U.: Protective role of lung surfactant protein D in a murine model of invasive pulmonary aspergillosis. Infect. Immun., 2001; 69: 2728-2731.
  5. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the folin phenol reagent. J. Biol. Chem., 1951; 193: 265-275.
  6. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970; 227: 680-685.
  7. Liau, D.F., Ryan, S.F.: Purification of surfactant protein A from dog lung by reconstitution with surfactant lipids. Chem. Phys. Lipids, 1991; 59: 29-38.
  8. Hospes, R., Hospes, B.I., Reiss, I., Bostedt, H., Gortner, L.: Molecular biological characterization of equine surfactant protein A. J. Vet. Med. A Physiol. Pathol. Clin. Med., 2002; 49: 497-498.
  9. White, R.T., Damm, D., Miller, J., Spratt, K., Schilling, J., Hawgood, S., Benson, B.: Cordell, B.: Isolation and characterization of the human pulmonary surfactant apoprotein gene. Nature, 1985; 317: 361-363.
  10. Katyal, S.L., Singh, G., Locker, J.: Characterization of a second human pulmonary surfactant-associated protein SP-A gene. Am. J. Respir. Cell Mol. Biol., 1992; 6: 446-452.
  11. Crouch, E.C.: Structure, biologic properties and expression of surfactant protein D (SP-D). Biochim. Biophys. Acta., 1998; 1408: 278-289.
  12. Wright, J.R.: Immunomodulatory functions of surfactant. Physiol. Rev., 1997; 77: 931-962.
  13. Sano, H., Kuroki, Y.: The lung collectins, SP-A and SP-D, modulate pulmonary innate immunity. Mol. Immunol., 2005; 42: 279-287.
  14. McCormack, F.X., Gibbons, R., Ward, S.R., Kuzmenko, A., Wu, H., Deepe, G.S. Jr.: Macrophage-independent fungicidal action of the pulmonary collectins. J. Biol. Chem., 2003; 278: 36250-36256.
  15. Alghamdi, A.S., Foster, D.N., Troedsson, M.H.T.: Equine seminal plasma reduces sperm binding to polymorphonuclear neutrophils (P